LOCUS       V041501                12631 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041501
VERSION     V041501
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2288..2314
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2318..2344
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2348..2374
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2381..6739
                     /label="KDM6A(NM_001291415)"
                     /note="KDM6A(NM_001291415)"
                     /gene="KDM6A"
     promoter        6790..7001
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7014..7282
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7311..7320
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7341..8003
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8073..8126
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8127..8726
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8727..9314
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9388..9621
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9693..9814
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9833..9968
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10006..10022)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10030..10046
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10054..10084)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10099..10120
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10408..10996)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11167..12027)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12028..12132)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12606..12622
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 caccatgtac ccatacgacg tacctgatta tgcaacttat ccttatgatg tacctgatta
     2341 tgctagctat ccttatgatg tcccagacta cgcagaattc atgaaatcct gcggagtgtc
     2401 gctcgctacc gccgccgctg ccgccgccgc tttcggtgat gaggaaaaga aaatggcggc
     2461 gggaaaagcg agcggcgaga gcgaggaggc gtcccccagc ctgacagccg aggagaggga
     2521 ggcgctcggc ggactggaca gccgcctctt tgggttcgtg agatttcatg aagatggcgc
     2581 caggacgaag gccctactgg gcaaggctgt tcgctgctat gaatctctaa tcttaaaagc
     2641 tgaaggaaaa gtggagtctg atttcttttg tcaattaggt cacttcaacc tcttattgga
     2701 agattatcca aaagcattat ctgcatacca gaggtactac agtttacagt ctgactactg
     2761 gaagaatgct gcctttttat atggtcttgg tttggtctac ttccattata atgcatttca
     2821 gtgggcaatt aaagcatttc aggaggtgct ttatgttgat cccagctttt gtcgagccaa
     2881 ggaaattcat ttacgacttg ggcttatgtt caaagtgaac acagactatg agtctagttt
     2941 aaagcatttt cagttagctt tggttgactg taatccctgc actttgtcca atgctgaaat
     3001 tcaatttcac attgcccact tatatgaaac ccagaggaaa tatcattctg caaaagaagc
     3061 ttatgaacaa cttttgcaga cagagaatct ttctgcacaa gtaaaagcaa ctgtcttaca
     3121 acagttaggt tggatgcatc acactgtaga tctcctggga gataaagcca ccaaggaaag
     3181 ctatgctatt cagtatctcc aaaagtcctt ggaagcagat cctaattctg gccagtcctg
     3241 gtatttcctc ggaaggtgct attcaagtat tgggaaagtt caggatgcct ttatatctta
     3301 caggcagtct attgataaat cagaagcaag tgcagataca tggtgttcaa taggtgtgct
     3361 atatcagcag caaaatcagc ccatggatgc tttacaggcc tatatttgtg ctgtacaatt
     3421 ggaccatggc catgctgcag cctggatgga cctaggcact ctctatgaat cctgcaacca
     3481 gcctcaggat gccattaaat gctacttaaa tgcaactaga agcaaaagtt gtagtaatac
     3541 ctctgcactt gcagcacgaa ttaagtattt acaggctcag ttgtgtaacc ttccacaagg
     3601 tagtctacag aataaaacta aattacttcc tagtattgag gaggcgtgga gcctaccaat
     3661 tcccgcagag cttacctcca ggcagggtgc catgaacaca gcacagcagg catgtaaacc
     3721 tcatcatcca aatactgaac ctgtattagg cctcagtcaa acaccaattt cacagcaatc
     3781 cttgccacta cacatgattc cttctagcca agtagatgac ctgtccagtc ctgccaagag
     3841 gaaaagaaca tctagtccaa caaagaatac ttctgacaat tggagtggtg gacatgctgt
     3901 gtcacatcct ccagtacagc aacaagctca ttcatggtgt ttgacaccac agaaattaca
     3961 gcatttggaa cagctccgcg caaatagaaa taatttaaat ccagcacaga aactgatgct
     4021 ggaacagctg gaaagtcagt ttgtcttaat gcaacaacac caaatgagac caacaggagt
     4081 tgcacaggta cgatctactg gaattcctaa tgggccaaca gctgactcat cactgcctac
     4141 aaactcagtc tctggccagc agccacagct tgctctgacc agagtgccta gcgtctctca
     4201 gcctggagtc cgtcctgcct gccctgggca gcctttggcc aatggaccct tttctgcagg
     4261 ccatgttccc tgtagcacat caagaacgct gggaagtaca gacactattt tgataggcaa
     4321 taatcatata acaggaagtg gaagtaatgg aaacgtgcct tacctgcagc gaaacgcact
     4381 cactctacct cataaccgca caaacctgac cagcagcgca gaggagccgt ggaaaaacca
     4441 actatctaac tccactcagg ggcttcacaa aggtcagagt tcacattcgg caggtcctaa
     4501 tggtgaacga cctctctctt ccactgggcc ttcccagcat ctccaggcag ctggctctgg
     4561 tattcagaat cagaacggac atcccaccct gcctagcaat tcagtaacac agggggctgc
     4621 tctcaatcac ctctcctctc acactgctac ctcaggtgga caacaaggca ttaccttaac
     4681 caaagagagc aagccttcag gaaacatatt gacggtgcct gaaacaagca ggcacactgg
     4741 agagacacct aacagcactg ccagtgtcga gggacttcct aatcatgtcc atcagatgac
     4801 ggcagatgct gtttgcagtc ctagccatgg agattctaag tcaccaggtt tactaagttc
     4861 agacaatcct cagctctctg ccttgttgat gggaaaagcc aataacaatg tgggtactgg
     4921 aacctgtgac aaagtcaata acatccaccc agctgttcat acaaagactg ataactctgt
     4981 tgcctcttca ccatcttcag ccatttcaac agcaacacct tctccaaaat ccactgagca
     5041 gacaaccaca aacagtgtta ccagccttaa cagccctcac agtgggctac acacaattaa
     5101 tggagaaggg atggaagaat ctcagagccc catgaaaaca gatctgcttc tggttaacca
     5161 caaacctagt ccacagatca taccatcaat gtctgtgtcc atatacccca gctcagcaga
     5221 agttctgaag gcatgcagga atctaggtaa aaatggctta tctaacagta gcattttgtt
     5281 ggataaatgt ccacctccaa gaccaccatc ttcaccatac cctcccttgc caaaggacaa
     5341 gttgaatcca cctacaccta gtatttactt ggaaaataaa cgtgatgctt tctttcctcc
     5401 attacatcaa ttttgtacaa atccgaacaa ccctgttaca gtaatacgtg gccttgctgg
     5461 agctcttaag ttagacctgg gacttttctc tactaaaact ttggtggaag ctaacaatga
     5521 acatatggta gaagtgagga cacagttgtt gcagccagca gatgaaaact gggatcccac
     5581 tggaacaaag aaaatctggc attgtgaaag taatagatct catactacaa ttgctaaata
     5641 tgcacagtac caggcctcct cattccagga atcattgaga gaagaaaatg aaaaaagaag
     5701 tcatcataaa gaccactcag atagtgaatc tacatcgtca gataattctg ggaggaggag
     5761 gaaaggaccc tttaaaacca taaagtttgg gaccaatatt gacctatctg atgacaaaaa
     5821 gtggaagttg cagctacatg agctgactaa acttcctgct tttgtgcgtg tcgtatcagc
     5881 aggaaatctt ctaagccatg ttggtcatac catattgggc atgaacacag ttcaactata
     5941 catgaaagtt ccagggagca gaacaccagg tcatcaggaa aataacaact tctgttcagt
     6001 taacataaat attggcccag gtgactgtga atggtttgtt gttcctgaag gttactgggg
     6061 tgttctgaat gacttctgtg aaaaaaataa tttgaatttc ctaatgggtt cttggtggcc
     6121 caatcttgaa gatctttatg aagcaaatgt tccagtgtat aggtttattc agcgacctgg
     6181 agatttggtc tggataaatg caggcactgt tcattgggtt caggctattg gctggtgcaa
     6241 caacattgct tggaatgttg gtccacttac agcctgccag tataaattgg cagtggaacg
     6301 gtacgaatgg aacaaattgc aaagtgtgaa gtcaatagta cccatggttc atctttcctg
     6361 gaatatggca cgaaatatca aggtctcaga tccaaagctt tttgaaatga ttaagtattg
     6421 tcttctaaga actctgaagc aatgtcagac attgagggaa gctctcattg ctgcaggaaa
     6481 agagattata tggcatgggc ggacaaaaga agaaccagct cattactgta gcatttgtga
     6541 agtggaggtt tttgatctgc tttttgtcac taatgagagt aattcacgaa agacctacat
     6601 agtacattgc caagattgtg cacgaaaaac aagcggaaac ttggaaaact ttgtggtgct
     6661 agaacagtac aaaatggagg acctgatgca agtctatgac caatttacat tagctcctcc
     6721 attaccatcc gcctcatctt gaggatccgc ggccgcgaag gatctgcgat cgctccggtg
     6781 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     6841 gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     6901 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     6961 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc
     7021 gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc
     7081 gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt
     7141 taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca
     7201 gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt
     7261 ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac gccaccatgg
     7321 agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc ggcaccctga
     7381 acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag ggccgcatga
     7441 ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg ctgagccacg
     7501 tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag aaccccttcc
     7561 tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac gaggacggcg
     7621 gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc ggcgacttca
     7681 aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag atcatccgca
     7741 gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg
     7801 cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac agccacatgc
     7861 acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg ttcgccttcc
     7921 gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac cagcacgcct
     7981 tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct gccgtggacg
     8041 gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt ctaacatgcg
     8101 gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg cgcctcgcca
     8161 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     8221 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     8281 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     8341 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     8401 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     8461 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     8521 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     8581 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     8641 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     8701 gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat
     8761 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     8821 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     8881 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     8941 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     9001 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     9061 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     9121 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     9181 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     9241 cggctctgcg gcctcttccg cgtctccgcc ttcgccctca gacgagtcgg atctcccttt
     9301 ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta
     9361 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaaataag
     9421 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     9481 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     9541 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     9601 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     9661 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     9721 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     9781 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     9841 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     9901 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     9961 tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat agctgtttcc
    10021 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
    10081 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
    10141 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
    10201 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    10261 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    10321 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    10381 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    10441 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    10501 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    10561 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    10621 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    10681 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    10741 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    10801 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
    10861 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    10921 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    10981 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    11041 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    11101 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    11161 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    11221 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    11281 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    11341 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    11401 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    11461 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    11521 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    11581 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    11641 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    11701 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    11761 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    11821 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    11881 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    11941 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    12001 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    12061 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    12121 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
    12181 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
    12241 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
    12301 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
    12361 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
    12421 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
    12481 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
    12541 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
    12601 acgttgtaaa acgacggcca gtgccaagct g
//
