LOCUS       V046871                11423 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046871
VERSION     V046871
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5508
                     /label="KDM4A(NM_014663)"
                     /note="KDM4A(NM_014663)"
                     /gene="KDM4A"
     CDS             5515..5538
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHHHH"
                     /label="8xHis"
     promoter        5582..5793
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5806..6074
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6103..6112
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6133..6795
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6865..6918
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6919..7518
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7519..8106
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8180..8413
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8485..8606
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8625..8760
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8798..8814)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8822..8838
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8846..8876)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8891..8912
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9200..9788)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9959..10819)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10820..10924)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11398..11414
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cttctgagtc tgaaactctg
     2341 aatcccagtg ctaggataat gaccttttat ccaactatgg aagagttccg aaacttcagt
     2401 agatacattg cctacattga atcccaagga gctcatcggg cagggctagc caaggttgtt
     2461 cctccaaaag agtggaagcc acgagcatcc tatgatgaca ttgatgattt ggtcattcct
     2521 gcccccattc aacagctggt gacggggcag tctggcctct ttactcagta caacatacag
     2581 aagaaagcca tgactgttcg agagttccgc aagatagcca atagcgataa gtactgtacc
     2641 ccacgctata gtgagtttga agagctcgag cggaaatact ggaaaaatct tacattcaat
     2701 cctccaatct atggtgcaga tgtgaatggt accctctatg aaaagcatgt tgatgagtgg
     2761 aatattggcc ggctgagaac aatcctggac ttggtggaaa aggagagtgg gatcaccatt
     2821 gagggtgtga acaccccata cctgtacttt ggcatgtgga agacatcctt tgcttggcac
     2881 actgaagaca tggacctcta cagcatcaac tacctgcact ttggagaacc aaagtcctgg
     2941 tactctgttc cacctgagca tggaaagcgg ttggaacgcc tcgccaaagg ctttttccca
     3001 ggaagtgctc aaagctgtga ggcatttctc cgccacaaga tgaccctgat ttccccgtta
     3061 atgctgaaga aatatggaat tccctttgac aaggtgactc aagaggctgg agagtttatg
     3121 atcactttcc cttatggtta ccatgccggc tttaaccatg gttttaactg tgcggagtct
     3181 accaattttg ctacccgtcg gtggattgag tacggcaagc aagctgtgct gtgctcctgt
     3241 agaaaggaca tggtgaagat ctccatggat gtgtttgtga gaaagttcca gccagaaagg
     3301 tacaaacttt ggaaagctgg gaaggacaac acagttattg accatactct gcccacgcca
     3361 gaagcagctg agtttcttaa ggagagtgaa ctgcctccaa gagctggcaa cgaggaggag
     3421 tgcccagagg aggacatgga aggggtggag gatggagagg aaggagacct gaagacaagc
     3481 ctggccaagc accgaatagg gacaaagagg caccgagttt gtcttgaaat accacaggag
     3541 gtgagtcaga gtgagctctt ccccaaggag gatctgagtt ctgagcagta tgagatgacg
     3601 gagtgcccgg cagccctcgc ccctgtgagg cccacccata gctctgtgcg gcaagttgag
     3661 gatggtctta ccttcccaga ttattctgac tccactgaag tcaaatttga agagcttaaa
     3721 aatgtcaaac tagaagagga ggatgaggag gaagaacaag cagcagctgc cttggatctt
     3781 tctgtgaatc ctgcgtctgt agggggacgc cttgtcttct caggctccaa aaagaaatca
     3841 tcttctagcc tgggctctgg ctcttcacgg gattctatct cttctgattc agaaactagt
     3901 gagcctctct cctgccgagc ccaagggcaa acgggagttc tcactgtgca cagttatgcc
     3961 aaaggggatg gcagggtcac tgtgggagag ccatgcacga ggaagaaagg aagcgccgct
     4021 agaagtttca gtgagcggga gctggcagag gttgcagatg aatacatgtt ttccctagaa
     4081 gagaataaga agtccaaggg acgccgtcag cctttaagca agctcccccg ccatcaccca
     4141 cttgtgctgc aggagtgtgt cagtgatgat gagacatctg aacagctgac ccctgaggaa
     4201 gaggctgagg agacagaggc ctgggccaag cctctgagcc aactgtggca gaaccgacct
     4261 ccaaactttg aggctgagaa ggaattcaat gagaccatgg cccaacaggc ccctcactgc
     4321 gctgtctgta tgatcttcca gacttatcat caggttgaat ttggaggctt taatcagaac
     4381 tgtggaaatg cttcagattt agccccccag aagcagagga ccaagccatt gattccagaa
     4441 atgtgcttca cttcgactgg ctgcagcacg gacatcaacc tttctactcc ttatcttgag
     4501 gaggatggca ccagcatact cgtttcctgc aagaagtgca gcgtccgggt ccatgccagt
     4561 tgctatgggg tcccccctgc aaaggcttct gaagactgga tgtgttctcg gtgttcagcc
     4621 aatgccctag aggaggactg ctgtttatgc tcattacgag gaggggccct gcagagagca
     4681 aatgatgaca ggtgggtcca cgtttcatgt gctgtggcaa ttctggaagc aaggtttgtc
     4741 aacattgcag aaagaagtcc ggtggatgtg agcaaaatcc ccctgccccg cttcaaactg
     4801 aaatgtatct tctgtaagaa gcggaggaaa agaactgctg gctgctgtgt gcagtgttct
     4861 cacggccgct gcccaactgc cttccatgtg agctgcgccc aggctgccgg tgtgatgatg
     4921 cagcctgacg actggccttt tgtggtcttc attacctgct ttcggcacaa gattcctaat
     4981 ttggagcgtg ccaagggggc cttgcaaagc atcactgcag gccagaaagt cattagcaag
     5041 cataagaacg ggcgcttcta ccagtgtgaa gtggtcaggc tcaccaccga gaccttctat
     5101 gaagtcaact ttgatgatgg ctccttcagc gacaatcttt atcctgagga catagtgagc
     5161 caggactgtc tccagtttgg tcctcctgct gaaggggaag tggtccaagt gagatggaca
     5221 gacggccaag tctatggagc caagtttgtg gcctcccacc ctatccaaat gtaccaggtg
     5281 gagtttgagg atggctcaca acttgtggtt aagagagatg atgtatacac actggatgaa
     5341 gagcttccca agagagtcaa atctagactg tcagtagcct cagacatgcg cttcaatgag
     5401 attttcacag agaaagaggt taagcaagaa aagaaacggc aacgagttat caactcaaga
     5461 taccgggaag attatattga gcctgcacta taccgggcca tcatggaggg atcccaccat
     5521 caccatcacc atcaccatta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     5581 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     5641 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     5701 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     5761 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     5821 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     5881 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     5941 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     6001 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     6061 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     6121 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     6181 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     6241 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     6301 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     6361 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     6421 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     6481 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     6541 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     6601 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     6661 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     6721 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     6781 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     6841 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     6901 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     6961 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     7021 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     7081 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     7141 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     7201 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     7261 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     7321 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     7381 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     7441 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     7501 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     7561 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     7621 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     7681 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7741 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7801 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7861 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     7921 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7981 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8041 cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttggccgcct
     8101 ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     8161 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt
     8221 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8281 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8341 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8401 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     8461 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     8521 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8581 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     8641 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     8701 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     8761 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa
     8821 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     8881 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     8941 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     9001 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     9061 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     9121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     9181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     9241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     9301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     9361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     9421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     9481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     9541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     9601 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     9661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     9841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9901 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    10021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    10081 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    10141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    10201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    10261 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    10321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    10381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    10441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    10501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    10561 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    10621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    10681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    10741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10801 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    10861 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    10921 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
    10981 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
    11041 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
    11101 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
    11161 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
    11221 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
    11281 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
    11341 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
    11401 aaacgacggc cagtgccaag ctg
//
