LOCUS       V041228                10961 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041228
VERSION     V041228
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5808
                     /label="Itgax(NM_021334)"
                     /note="Itgax(NM_021334)"
                     /gene="Itgax"
     CDS             5815..5880
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5931..6142
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6155..6423
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6450..7049
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7056..7644
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7718..7951
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8023..8144
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8163..8298
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8336..8352)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8360..8376
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8384..8414)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8429..8450
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8738..9326)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9497..10357)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10358..10462)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10936..10952
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgagctgt acctggatag cctttcttct gctgttgggg
     2341 tttgtttctt gtcttggctt caacttggat gcagagaagc tgacacattt tcacatggac
     2401 ggtgctgagt tcggacacag tgtgctccag tatgatagtt cctgggtggt ggttggagca
     2461 ccaaaggaaa taaaagccac taaccaaata ggtggcctct acaaatgtgg ctatcacaca
     2521 ggcaactgtg agcccatctc cctccaggtg cccccagagg ctgtgaacat atccctgggc
     2581 ctgtcccttg ctgctgccac caacccttcc tggctgttgg cttgtggtcc tactgtgcac
     2641 cacacatgca gagagaatat atacttgaca gggctctgct ttctactgag ttcatcattc
     2701 aagcagagcc agaacttccc aactgcacag caggagtgtc caaagcaaga ccaagacatc
     2761 gtgttcctga ttgatggctc gggtagcatc agttccacag attttgaaaa aatgctggac
     2821 tttgttaaag ctgtgatgag ccagcttcag agacctagca cacggttctc cctgatgcag
     2881 ttctctgatt acttccgagt acattttact ttcaacaact tcatctccac gtcaagccct
     2941 ttaagtctgt tgggttctgt aaggcagcta agagggtaca catacacagc ctcggctatc
     3001 aagcatgtca taacagaact gttcaccacc caaagtggag ctcggcaaga tgccaccaag
     3061 gtcctcattg tcatcactga tgggagaaaa caaggggaca acttgagtta tgatagtgtc
     3121 atccccatgg cagaggctgc aagcatcatt cgttatgcaa ttggggtagg aaaggccttt
     3181 tacaatgaac attccaagca agaattaaag gccattgcat cgatgccttc ccatgaatac
     3241 gtattcagcg tggagaactt tgatgctttg aaggatattg agaatcagct gaaggagaag
     3301 atctttgcca ttgagggcac agagacacca agcagcagta cttttgaatt ggagatgtcc
     3361 caggagggct tcagtgctgt gtttacacct gatggaccag ttctgggggc tgtgggaagc
     3421 ttcagctggt ctggaggtgc cttcttgtac ccctcaaata tgagacccac cttcatcaac
     3481 atgtctcagg agaacgagga catgagggac gcttacctgg gttactccac cgcactggcc
     3541 ttttggaagg gggtccacag cctgatcctg ggggcccctc gccaccagca cacggggaag
     3601 gttgtcatct ttacccagga atccaggcac tggaggccca agtctgaagt cagagggaca
     3661 cagatcggct cctactttgg ggcatctctt tgttctgtgg acatggatag agatggcagc
     3721 actgacctgg tcctgattgg agtcccccat tactatgagc acacccgagg ggggcaggtg
     3781 tcagtgtgcc ccatgcccgg tgtggggagc aggtggcatt gtgggaccac cctccatggg
     3841 gagcagggcc atccttgggg ccgctttggg gcggctctga cagtgctagg ggacgtgaat
     3901 ggggacagtc tggcagatgt ggctattggt gcacccggag aggaggagaa cagaggtgct
     3961 gtctacatat ttcatggagc ctcaagacag gacatcgctc cctcacccag ccagaggatt
     4021 tcagcatccc agatcccctc caggattcaa tattttgggc agtcactgag tgggggccag
     4081 gacctcaccc gggatggact ggtggatctg gccgtgggct ccaagggacg cgtgctgctg
     4141 ctcaggacca gaccaattct tagagtgtca ccaactgttc acttcacacc tgcagagatt
     4201 tccaggtctg tgtttgagtg tcaggagcag gtggcccctg agcagacact gagtgatgcc
     4261 actgtctgcc ttcatattca tgaaagcccc aagacccaac taggtgacct ccgaagtact
     4321 gtcacctttg acctggctct agaccatggc cgtctgagta cccgggccat cttcaaggag
     4381 acaaagaccc gggctctgac tcgagttaaa actcttggtc tgaacaagca ctgtgaaagt
     4441 gtgaagttgc ttctcccggc ctgtgtggag gactcagtga ccccgatcac tcttcgcctc
     4501 aacttttctc ttgttggtgt gcccatcagt tccttacaaa atctccaacc catgctggct
     4561 gtagatgacc aaacgtactt cacggcctct cttccctttg agaagaactg tggagctgac
     4621 cacatctgcc aggatgacct tagtgtcgta tttggcttcc cagacttgaa gaccctggtg
     4681 gtgggaagtg acctggagct gaatgtagat gtgacggtgt ctaatgatgg agaagattcc
     4741 tacggaacca cagtcactct gttctacccc gtgggtctgt cctttagacg agttgcagaa
     4801 ggccaagtat tccttcggaa gaaagaggac cagcagtggc agcggcgtgg gcagcactcc
     4861 ctgcacctca tgtgtgacag caccccagac aggagtcagg gcctctggag taccagctgt
     4921 agcagcagac atgtcatttt ccgtggaggc tcacagatga cttttttggt aacttttgac
     4981 gtctccccca aagctgagct gggggaccgg ctgcttctaa gagccagagt gggaagtgag
     5041 aataatgtac ccggaacccc aaaaaccacc ttccagttgg agcttccagt aaaatatgcg
     5101 gtgtacacca tgatcagcag ccatgaccag tttaccaagt atcttaactt ctcaacctca
     5161 gaaaaggaga agaccagtgt ggtcgaacat agattccagg tgaataacct gggccagaga
     5221 gacgtgccag tcagcatcaa cttttgggtg cccatagagc tgaagggaga agctgtgtgg
     5281 acagtgatgg tctcccaccc tcagaaccca ctcacccaat gctatcggaa tagactcaag
     5341 ccaacacaat ttgacctcct gactcacatg caaaagagtc ccgtgctgga ctgctccatc
     5401 gctgactgcc tgcacctccg ctgtgacatc ccctccttgg gcatcctgga tgagctttac
     5461 ttcattctga agggcaacct cagcttcggc tggatcagtc agacattgca gaaaaaggtg
     5521 ttgctcctga gtgaggctga aatcacattc aacacatctg tgtattccca gctgccggga
     5581 caggaggcat ttctgagagc ccagacgaag acagtgctgg agatgtataa agttcacaac
     5641 cccgtccctc ttatcgtggg cagctcagtg ggaggtctgc tgctgctggc tatcatcaca
     5701 gctatacttt acaaggctgg tttcttcaaa cgtcagtaca aggagatgtt ggaggaagca
     5761 aatggacagt ttgtctcaga cgggacccca actccccagg ttgcccagct cgaggactac
     5821 aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag
     5881 tagggatccg cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg
     5941 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta
     6001 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     6061 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     6121 cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc
     6181 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc
     6241 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc
     6301 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg
     6361 cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag
     6421 atccaagctg tgaccggcgc ctacgctaga tgaccgagta caagcccacg gtgcgcctcg
     6481 ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc
     6541 ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag
     6601 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     6661 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     6721 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     6781 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     6841 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     6901 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     6961 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     7021 ggtgcatgac ccgcaagccc ggtgcctgag tcgacaatca acctctggat tacaaaattt
     7081 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     7141 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     7201 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     7261 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     7321 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     7381 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     7441 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     7501 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     7561 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     7621 tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct
     7681 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     7741 cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     7801 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7861 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7921 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     7981 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     8041 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     8101 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     8161 ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     8221 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     8281 gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat
     8341 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     8401 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8461 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     8521 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8581 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8641 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8701 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8761 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8821 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8881 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8941 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     9001 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     9061 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9121 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     9181 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9241 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9301 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9361 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9421 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9481 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9541 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9601 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9661 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9721 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9781 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     9841 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9901 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9961 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    10021 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    10081 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    10141 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10201 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10261 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10321 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10381 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10441 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    10501 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
    10561 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
    10621 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
    10681 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
    10741 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
    10801 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
    10861 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
    10921 cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
//
