LOCUS       V028706                11347 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028706
VERSION     V028706
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5373
                     /label="Ide(NM_031156)"
                     /note="Ide(NM_031156)"
                     /gene="Ide"
     CDS             5380..5445
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5489..5700
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5713..5981
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6010..6019
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6040..6702
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6772..6825
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6826..7425
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7426..8014
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8088..8321
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8393..8514
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8554..8689
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8722..8738)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8746..8762
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8770..8800)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8815..8836
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9124..9712)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9883..10743)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10744..10848)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11322..11338
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc ggaacgggct cgtgtggctg
     2341 ctgcaccccg cgctgcccgg caccttgcgc tccatcctcg gcgcccgccc gccgcccgcg
     2401 aagcgactgt gtggattccc aaaacagact tacagcacaa tgagtaatcc ggccatccag
     2461 agaatagaag accaaattgt caagtctcct gaagacaaac gggaataccg tggactagag
     2521 ctggccaatg gcatcaaagt gcttctcatc agcgatccca ccacagacaa gtcctcagcg
     2581 gccctcgatg tgcacatagg ttcactgtca gaccctccaa atattcctgg cttaagtcat
     2641 ttttgtgaac atatgctgtt tttgggaacc aagaaatatc ctaaagaaaa tgaatatagc
     2701 cagtttctca gtgaacatgc tggaagttca aatgcattca ccagtggaga acacaccaat
     2761 tattatttcg atgtttccca tgaacacttg gaaggagccc tggacaggtt tgcgcagttt
     2821 ttcctgtgcc ccttgtttga tgcaagttgt aaagacagag aggtgaacgc tgtcgattca
     2881 gaacatgaga agaatgtgat gaacgatgcc tggagactct tccagctgga aaaggctacg
     2941 gggaacccca aacacccctt cagcaaattt gggacaggaa acaaatatac tctagagact
     3001 cggcccaacc aagaaggcat cgacgtaagg gaagagctct tgaaatttca ctctacgtat
     3061 tattcgtcca atctgatggc gatttgtgtt ttaggtcgag aatccttaga cgacctcact
     3121 aacctggtgg tgaagctgtt ctccgaggtg gagaacaaga acgttcctct gcctgagttc
     3181 cctgagcacc ctttccagga agagcatctc aggcaacttt ataaaatagt acccattaag
     3241 gatattagaa atctttatgt gacatttccc ataccagacc ttcagcagta ctacaaatcc
     3301 aatcctggtc attatcttgg tcatctaatt gggcacgaag gtcctggaag cctgttgtca
     3361 gaactcaaat caaagggctg ggtgaatacc ctggttggtg gacagaagga aggagcccga
     3421 ggttttatgt tttttatcat taatgtggac ttaactgaag aagggttatt gcacgttgaa
     3481 gatataattt tgcacatgtt tcaatacatt cagaagctac gtgcagaagg acctcaagaa
     3541 tgggttttcc aagagtgcaa ggacttgaat gctgttgctt tcagatttaa agataaagag
     3601 agaccacgag gctatacgtc caagattgca ggaaagttgc actattatcc cctaaatgga
     3661 gtgctcacag ctgaatatct attggaggaa tttagacccg acttgataga catggttctt
     3721 gataaactca gaccagaaaa tgtgcgggtg gcaatagttt ccaaatcatt tgaagggaaa
     3781 actgaccgca cagagcagtg gtatggaaca cagtataagc aagaagctat cccagaggac
     3841 atcattcaga aatggcaaaa tgctgacctt aatggaaaat ttaagcttcc aacaaagaat
     3901 gaatttattc ccacaaattt tgagattttg tcattagaaa aagatgcaac accataccct
     3961 gctcttatta aggatacagc catgagtaag ctgtggttca aacaagatga taaatttttc
     4021 ttgccgaaag cttgtctcaa ctttgaattt ttcagcccgt ttgcttatgt ggaccccttg
     4081 cactgtaaca tggcctattt gtaccttgag ctcctcaaag actcactcaa cgagtatgca
     4141 tatgcagcag agctagcagg cctgagctat gacctccaaa ataccatcta tgggatgtat
     4201 ctctcagtga aaggttacaa tgacaaacag ccaattttgc tgaagaagat caccgagaaa
     4261 atggctactt ttgagattga taagaagaga tttgaaatta tcaaagaggc ctatatgcga
     4321 tctcttaata acttccgggc tgagcagcct caccagcatg ccatgtacta cctccgcttg
     4381 ctgatgactg aagtggcctg gactaaagat gagctaaaag aagcccttga cgatgttacc
     4441 ctcccccgac ttaaggcctt catacctcag ctgctgtcac ggctgcatat tgaagcactt
     4501 ctccatggaa acataaccaa gcaggctgcc ttaggagtta tgcagatggt agaagacact
     4561 cttatcgagc atgctcacac caaacctctc cttccaagtc agctagtccg gtacagagaa
     4621 gtccagctcc ctgacagagg atggtttgtt tatcagcaga ggaatgaagt tcacaataac
     4681 tgcggcatcg agatctacta ccagacagac atgcagagca cctcggagaa catgttcctg
     4741 gagctgtttt gccagattat ctcggagcct tgcttcaata ccctgcgcac caaggagcag
     4801 cttggctata ttgtcttcag tggaccccgt cgggccaacg gtatccaagg cttgcgcttc
     4861 atcatccagt cagaaaaacc accccactac ctggagagca gagtagaagc cttcttgatt
     4921 accatggaaa aggccataga ggacatgaca gaggaggcgt tccaaaaaca cattcaagca
     4981 ctagcgattc ggcgactcga caaaccaaag aaactctctg cggagtgtgc taaatactgg
     5041 ggggaaatca tctcgcagca gtacaattac gacagagata acatagaagt tgcatattta
     5101 aagacactta ccaaggacga tatcatcaga ttctaccagg aaatgttggc tgtcgacgca
     5161 ccgaggagac ataaagtgtc tgtccatgtt cttgccaggg aaatggattc ttgtcctgtt
     5221 gttggagagt tcccctctca gaatgatata aacttgtccg aagcgccacc cttgccacaa
     5281 cctgaagtga ttcacaacat gactgagttc aagcgtggcc tgccactgtt tcccctcgtg
     5341 aaaccacata ttaacttcat ggctgcaaaa ctcggatccg actacaaaga ccatgacggt
     5401 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     5461 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     5521 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     5581 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     5641 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     5701 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     5761 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     5821 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     5881 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     5941 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     6001 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     6061 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     6121 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     6181 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     6241 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     6301 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     6361 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     6421 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     6481 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     6541 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     6601 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     6661 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     6721 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     6781 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     6841 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6901 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6961 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     7021 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     7081 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     7141 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     7201 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     7261 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     7321 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     7381 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     7441 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7501 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7561 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7621 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7681 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7741 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7801 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     7861 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7921 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     7981 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta
     8041 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     8101 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca
     8161 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8221 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8281 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     8341 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     8401 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8461 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     8521 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8581 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8641 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
     8701 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8761 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8821 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8881 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8941 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     9001 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9061 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9121 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9181 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9241 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9301 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9361 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9421 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9481 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9541 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     9601 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9661 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9721 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9781 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9841 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9901 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9961 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    10021 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    10081 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10141 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    10201 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10261 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10321 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10381 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10441 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10501 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10561 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10621 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10681 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10741 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10801 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10861 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    10921 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    10981 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    11041 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
    11101 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
    11161 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
    11221 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    11281 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
    11341 caagctg
//
