LOCUS       V027898                10216 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027898
VERSION     V027898
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4242
                     /label="IVNS1ABP(NM_006469)"
                     /note="IVNS1ABP(NM_006469)"
                     /gene="IVNS1ABP"
     CDS             4249..4314
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4358..4569
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4582..4850
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4879..4888
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4909..5571
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5641..5694
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5695..6294
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6295..6883
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6957..7190
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7262..7383
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7423..7558
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7591..7607)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7615..7631
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7639..7669)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7684..7705
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7993..8581)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8752..9612)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9613..9717)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10191..10207
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga ttcccaatgg atatttgatg
     2341 tttgaggatg aaaattttat tgagtcttct gttgccaaat taaatgccct gaggaaaagt
     2401 ggccagttct gtgatgttcg acttcaggtc tgtggccatg aaatgttagc acacagagca
     2461 gtgctagctt gctgcagtcc ctatttattt gaaatcttta atagtgatag tgatcctcat
     2521 ggaatttctc acgttaaatt tgatgatctc aatccagaag ctgttgaagt cttgttgaat
     2581 tatgcctaca ctgctcagtt gaaagcagat aaggaattgg taaaagatgt ttattctgca
     2641 gcaaaaaagc tgaagatgga tcgagtaaag caggtttgtg gtgattattt actgtctaga
     2701 atggatgtta ccagctgcat ctcttaccga aattttgcaa gttgtatggg agactcccgt
     2761 ttgttgaata aggttgatgc ttatattcag gagcatttgt tacaaatttc tgaagaggag
     2821 gagtttctta agcttccaag gctaaagttg gaggtaatgc ttgaagataa tgtttgcttg
     2881 cccagcaatg gcaaattata tacaaaggta atcaactggg tgcagcgtag catctgggag
     2941 aatggagaca gtctggaaga gctgatggaa gaggttcaaa ccttgtacta ctcagctgat
     3001 cacaagctgc ttgatgggaa cctactagat ggacaggctg aggtgtttgg cagtgatgat
     3061 gaccacattc agtttgtgca gaaaaagcca ccacgtgaga atggccataa gcagataagt
     3121 agcagttcaa ctggatgtct ctcttctcca aatgctacag tacaaagccc taagcatgag
     3181 tggaaaatcg ttgcttcaga aaagacttca aataacactt acttgtgcct ggctgtgctg
     3241 gatggtatat tctgtgtcat ttttcttcat gggagaaaca gcccacagag ctcaccaaca
     3301 agtactccaa aactaagtaa gagtttaagc tttgagatgc aacaagatga gctaatcgaa
     3361 aagcccatgt ctcctatgca gtacgcacga tctggtctgg gaacagcaga gatgaatggc
     3421 aaactcatag ctgcaggtgg ctataacaga gaggaatgtc ttcgaacagt cgaatgctat
     3481 aatccacata cagatcactg gtcctttctt gctcccatga gaacaccaag agcccgattt
     3541 caaatggctg tactcatggg ccagctctat gtggtaggtg gatcaaatgg ccactcagat
     3601 gacctgagtt gtggagagat gtatgattca aacatagatg actggattcc tgttccagaa
     3661 ttgagaacta accgttgtaa tgcaggagtg tgtgctctga atggaaagtt atacatcgtt
     3721 ggtggctctg atccatatgg tcaaaaagga ctgaaaaatt gtgatgtatt tgatcctgta
     3781 acaaagttgt ggacaagctg tgcccctctt aacattcgga gacaccagtc tgcagtctgt
     3841 gagcttggtg gttatttgta cataatcgga ggtgcagaat cttggaattg tctgaacaca
     3901 gtagaacgat acaatcctga aaataatacc tggactttaa ttgcacccat gaatgtggct
     3961 aggcgaggag ctggagtggc tgttcttaat ggaaaactgt ttgtatgtgg tggctttgat
     4021 ggttctcatg ccatcagttg tgtggaaatg tatgatccaa ctagaaatga atggaagatg
     4081 atgggaaata tgacttcacc aaggagcaat gctgggattg caactgtagg gaacaccatt
     4141 tatgcagtgg gaggattcga tggcaatgaa tttctgaata cggtggaagt ctataacctt
     4201 gagtcaaatg aatggagccc ctatacaaag attttccagt ttggatccga ctacaaagac
     4261 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagcgg
     4321 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     4381 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     4441 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     4501 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     4561 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     4621 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     4681 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     4741 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     4801 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     4861 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag
     4921 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga
     4981 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg
     5041 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc
     5101 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac
     5161 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac
     5221 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc
     5281 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg
     5341 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac
     5401 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg
     5461 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag
     5521 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc
     5581 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc
     5641 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     5701 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     5761 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     5821 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     5881 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     5941 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     6001 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6061 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6121 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6181 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6241 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa
     6301 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6361 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6421 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6481 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6541 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6601 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6661 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     6721 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6781 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6841 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc
     6901 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     6961 agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg
     7021 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7081 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7141 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7201 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     7261 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7321 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7381 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     7441 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7501 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7561 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     7621 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7681 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7741 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7801 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7861 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7921 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7981 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8041 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8101 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8161 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8221 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8281 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8341 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8401 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     8461 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8521 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8581 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8641 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8701 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8761 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8821 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8881 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8941 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9001 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9061 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9121 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9181 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9241 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9301 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9361 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9421 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9481 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9541 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9601 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9661 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9721 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     9781 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     9841 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     9901 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     9961 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    10021 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
    10081 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    10141 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    10201 ggccagtgcc aagctg
//
