LOCUS       V032355                10907 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032355
VERSION     V032355
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2287..2352
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             2377..5832
                     /label="ITGAM(NM_001145808)"
                     /note="ITGAM(NM_001145808)"
                     /gene="ITGAM"
     promoter        5877..6088
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6101..6369
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6396..6995
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7002..7590
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7664..7897
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7969..8090
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8109..8244
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8282..8298)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8306..8322
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8330..8360)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8375..8396
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8684..9272)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9443..10303)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10304..10408)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10882..10898
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agaatggact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat
     2341 gacgatgaca agtctagaga attcggatcc gccaccatgg ctctcagagt ccttctgtta
     2401 acagccttga ccttatgtca tgggttcaac ttggacactg aaaacgcaat gaccttccaa
     2461 gagaacgcaa ggggcttcgg gcagagcgtg gtccagcttc agggatccag ggtggtggtt
     2521 ggagcccccc aggagatagt ggctgccaac caaaggggca gcctctacca gtgcgactac
     2581 agcacaggct catgcgagcc catccgcctg caggtccccg tggaggccgt gaacatgtcc
     2641 ctgggcctgt ccctggcagc caccaccagc ccccctcagc tgctggcctg tggtcccacc
     2701 gtgcaccaga cttgcagtga gaacacgtat gtgaaagggc tctgcttcct gtttggatcc
     2761 aacctacggc agcagcccca gaagttccca gaggccctcc gagggtgtcc tcaagaggat
     2821 agtgacattg ccttcttgat tgatggctct ggtagcatca tcccacatga ctttcggcgg
     2881 atgaaggagt ttgtctcaac tgtgatggag caattaaaaa agtccaaaac cttgttctct
     2941 ttgatgcagt actctgaaga attccggatt cactttacct tcaaagagtt ccagaacaac
     3001 cctaacccaa gatcactggt gaagccaata acgcagctgc ttgggcggac acacacggcc
     3061 acgggcatcc gcaaagtggt acgagagctg tttaacatca ccaacggagc ccgaaagaat
     3121 gcctttaaga tcctagttgt catcacggat ggagaaaagt ttggcgatcc cttgggatat
     3181 gaggatgtca tccctgaggc agacagagag ggagtcattc gctacgtcat tggggtggga
     3241 gatgccttcc gcagtgagaa atcccgccaa gagcttaata ccatcgcatc caagccgcct
     3301 cgtgatcacg tgttccaggt gaataacttt gaggctctga agaccattca gaaccagctt
     3361 cgggagaaga tctttgcgat cgagggtact cagacaggaa gtagcagctc ctttgagcat
     3421 gagatgtctc aggaaggctt cagcgctgcc atcacctcta atggcccctt gctgagcact
     3481 gtggggagct atgactgggc tggtggagtc tttctatata catcaaagga gaaaagcacc
     3541 ttcatcaaca tgaccagagt ggattcagac atgaatgatg cttacttggg ttatgctgcc
     3601 gccatcatct tacggaaccg ggtgcaaagc ctggttctgg gggcacctcg atatcagcac
     3661 atcggcctgg tagcgatgtt caggcagaac actggcatgt gggagtccaa cgctaatgtc
     3721 aagggcaccc agatcggcgc ctacttcggg gcctccctct gctccgtgga cgtggacagc
     3781 aacggcagca ccgacctggt cctcatcggg gccccccatt actacgagca gacccgaggg
     3841 ggccaggtgt ccgtgtgccc cttgcccagg gggagggctc ggtggcagtg tgatgctgtt
     3901 ctctacgggg agcagggcca accctggggc cgctttgggg cagccctaac agtgctgggg
     3961 gacgtaaatg gggacaagct gacggacgtg gccattgggg ccccaggaga ggaggacaac
     4021 cggggtgctg tttacctgtt tcacggaacc tcaggatctg gcatcagccc ctcccatagc
     4081 cagcggatag caggctccaa gctctctccc aggctccagt attttggtca gtcactgagt
     4141 gggggccagg acctcacaat ggatggactg gtagacctga ctgtaggagc ccaggggcac
     4201 gtgctgctgc tcaggtccca gccagtactg agagtcaagg caatcatgga gttcaatccc
     4261 agggaagtgg caaggaatgt atttgagtgt aatgatcagg tggtgaaagg caaggaagcc
     4321 ggagaggtca gagtctgcct ccatgtccag aagagcacac gggatcggct aagagaagga
     4381 cagatccaga gtgttgtgac ttatgacctg gctctggact ccggccgccc acattcccgc
     4441 gccgtcttca atgagacaaa gaacagcaca cgcagacaga cacaggtctt ggggctgacc
     4501 cagacttgtg agaccctgaa actacagttg ccgaattgca tcgaggaccc agtgagcccc
     4561 attgtgctgc gcctgaactt ctctctggtg ggaacgccat tgtctgcttt cgggaacctc
     4621 cggccagtgc tggcggagga tgctcagaga ctcttcacag ccttgtttcc ctttgagaag
     4681 aattgtggca atgacaacat ctgccaggat gacctcagca tcaccttcag tttcatgagc
     4741 ctggactgcc tcgtggtggg tgggccccgg gagttcaacg tgacagtgac tgtgagaaat
     4801 gatggtgagg actcctacag gacacaggtc accttcttct tcccgcttga cctgtcctac
     4861 cggaaggtgt ccacgctcca gaaccagcgc tcacagcgat cctggcgcct ggcctgtgag
     4921 tctgcctcct ccaccgaagt gtctggggcc ttgaagagca ccagctgcag cataaaccac
     4981 cccatcttcc cggaaaactc agaggtcacc tttaatatca cgtttgatgt agactctaag
     5041 gcttcccttg gaaacaaact gctcctcaag gccaatgtga ccagtgagaa caacatgccc
     5101 agaaccaaca aaaccgaatt ccaactggag ctgccggtga aatatgctgt ctacatggtg
     5161 gtcaccagcc atggggtctc cactaaatat ctcaacttca cggcctcaga gaataccagt
     5221 cgggtcatgc agcatcaata tcaggtcagc aacctggggc agaggagcct ccccatcagc
     5281 ctggtgttct tggtgcccgt ccggctgaac cagactgtca tatgggaccg cccccaggtc
     5341 accttctccg agaacctctc gagtacgtgc cacaccaagg agcgcttgcc ctctcactcc
     5401 gactttctgg ctgagcttcg gaaggccccc gtggtgaact gctccatcgc tgtctgccag
     5461 agaatccagt gtgacatccc gttctttggc atccaggaag aattcaatgc taccctcaaa
     5521 ggcaacctct cgtttgactg gtacatcaag acctcgcata accacctcct gatcgtgagc
     5581 acagctgaga tcttgtttaa cgattccgtg ttcaccctgc tgccgggaca gggggcgttt
     5641 gtgaggtccc agacggagac caaagtggag ccgttcgagg tccccaaccc cctgccgctc
     5701 atcgtgggca gctctgtcgg gggactgctg ctcctggccc tcatcaccgc cgcgctgtac
     5761 aagctcggct tcttcaagcg gcaatacaag gacatgatga gtgaaggggg tcccccgggg
     5821 gccgaacccc agtaagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc
     5881 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
     5941 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     6001 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     6061 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
     6121 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
     6181 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
     6241 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
     6301 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
     6361 ttacagatcc aagctgtgac cggcgcctac gctagatgac cgagtacaag cccacggtgc
     6421 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     6481 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     6541 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     6601 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     6661 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     6721 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     6781 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     6841 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     6901 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     6961 gcacctggtg catgacccgc aagcccggtg cctgagtcga caatcaacct ctggattaca
     7021 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     7081 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     7141 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7201 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     7261 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     7321 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     7381 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     7441 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7501 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     7561 gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat gacttacaag
     7621 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7681 tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7741 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7801 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7861 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     7921 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     7981 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8041 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     8101 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8161 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     8221 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat
     8281 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8341 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8401 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8461 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     8521 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8581 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8641 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8701 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8761 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8821 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8881 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8941 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9001 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9061 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9121 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9181 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9241 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9301 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9361 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9421 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9481 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9541 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9601 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9661 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9721 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9781 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9841 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9901 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9961 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10021 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10081 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10141 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10201 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10261 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10321 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10381 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
    10441 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
    10501 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
    10561 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
    10621 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
    10681 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
    10741 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
    10801 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
    10861 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
