LOCUS       V024572                11285 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024572
VERSION     V024572
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        131..334
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             396..3542
                     /label="ITGA5(NM_002205)"
                     /note="ITGA5(NM_002205)"
                     /gene="ITGA5"
     CDS             3549..3614
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             3618..4328
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     misc_feature    4368..4485
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        4534..5044
                     /note="human phosphoglycerate kinase 1 promoter"
                     /label="hPGK promoter"
     CDS             5066..5665
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     LTR             5793..6026
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6098..6219
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6259..6394
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        complement(6415..6433)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(6443..6459)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     rep_origin      6601..7056
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7082..7186
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7187..8047
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8218..8806
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     protein_bind    9094..9115
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        9130..9160
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9168..9184
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     9192..9208
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     promoter        9229..9247
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     promoter        9275..9501
                     /note="Rous sarcoma virus enhancer/promoter"
                     /label="RSV promoter"
     LTR             9502..9682
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    9729..9854
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    10347..10580
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             10765..10809
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcgctaccgg tgatatcacg cgtgtcgacg ccaccatggg gagccggacg ccagagtccc
      421 ctctccacgc cgtgcagctg cgctggggcc cccggcgccg acccccgctg ctgccgctgc
      481 tgttgctgct gctgccgccg ccacccaggg tcgggggctt caacttagac gcggaggccc
      541 cagcagtact ctcggggccc ccgggctcct tcttcggatt ctcagtggag ttttaccggc
      601 cgggaacaga cggggtcagt gtgctggtgg gagcacccaa ggctaatacc agccagccag
      661 gagtgctgca gggtggtgct gtctacctct gtccttgggg tgccagcccc acacagtgca
      721 cccccattga atttgacagc aaaggctctc ggctcctgga gtcctcactg tccagctcag
      781 agggagagga gcctgtggag tacaagtcct tgcagtggtt cggggcaaca gttcgagccc
      841 atggctcctc catcttggca tgcgctccac tgtacagctg gcgcacagag aaggagccac
      901 tgagcgaccc cgtgggcacc tgctacctct ccacagataa cttcacccga attctggagt
      961 atgcaccctg ccgctcagat ttcagctggg cagcaggaca gggttactgc caaggaggct
     1021 tcagtgccga gttcaccaag actggccgtg tggttttagg tggaccagga agctatttct
     1081 ggcaaggcca gatcctgtct gccactcagg agcagattgc agaatcttat taccccgagt
     1141 acctgatcaa cctggttcag gggcagctgc agactcgcca ggccagttcc atctatgatg
     1201 acagctacct aggatactct gtggctgttg gtgaattcag tggtgatgac acagaagact
     1261 ttgttgctgg tgtgcccaaa gggaacctca cttacggcta tgtcaccatc cttaatggct
     1321 cagacattcg atccctctac aacttctcag gggaacagat ggcctcctac tttggctatg
     1381 cagtggccgc cacagacgtc aatggggacg ggctggatga cttgctggtg ggggcacccc
     1441 tgctcatgga tcggacccct gacgggcggc ctcaggaggt gggcagggtc tacgtctacc
     1501 tgcagcaccc agccggcata gagcccacgc ccacccttac cctcactggc catgatgagt
     1561 ttggccgatt tggcagctcc ttgacccccc tgggggacct ggaccaggat ggctacaatg
     1621 atgtggccat cggggctccc tttggtgggg agacccagca gggagtagtg tttgtatttc
     1681 ctgggggccc aggagggctg ggctctaagc cttcccaggt tctgcagccc ctgtgggcag
     1741 ccagccacac cccagacttc tttggctctg cccttcgagg aggccgagac ctggatggca
     1801 atggatatcc tgatctgatt gtggggtcct ttggtgtgga caaggctgtg gtatacaggg
     1861 gccgccccat cgtgtccgct agtgcctccc tcaccatctt ccccgccatg ttcaacccag
     1921 aggagcggag ctgcagctta gaggggaacc ctgtggcctg catcaacctt agcttctgcc
     1981 tcaatgcttc tggaaaacac gttgctgact ccattggttt cacagtggaa cttcagctgg
     2041 actggcagaa gcagaaggga ggggtacggc gggcactgtt cctggcctcc aggcaggcaa
     2101 ccctgaccca gaccctgctc atccagaatg gggctcgaga ggattgcaga gagatgaaga
     2161 tctacctcag gaacgagtca gaatttcgag acaaactctc gccgattcac atcgctctca
     2221 acttctcctt ggacccccaa gccccagtgg acagccacgg cctcaggcca gccctacatt
     2281 atcagagcaa gagccggata gaggacaagg ctcagatctt gctggactgt ggagaagaca
     2341 acatctgtgt gcctgacctg cagctggaag tgtttgggga gcagaaccat gtgtacctgg
     2401 gtgacaagaa tgccctgaac ctcactttcc atgcccagaa tgtgggtgag ggtggcgcct
     2461 atgaggctga gcttcgggtc accgcccctc cagaggctga gtactcagga ctcgtcagac
     2521 acccagggaa cttctccagc ctgagctgtg actactttgc cgtgaaccag agccgcctgc
     2581 tggtgtgtga cctgggcaac cccatgaagg caggagccag tctgtggggt ggccttcggt
     2641 ttacagtccc tcatctccgg gacactaaga aaaccatcca gtttgacttc cagatcctca
     2701 gcaagaatct caacaactcg caaagcgacg tggtttcctt tcggctctcc gtggaggctc
     2761 aggcccaggt caccctgaac ggtgtctcca agcctgaggc agtgctattc ccagtaagcg
     2821 actggcatcc ccgagaccag cctcagaagg aggaggacct gggacctgct gtccaccatg
     2881 tctatgagct catcaaccaa ggccccagct ccattagcca gggtgtgctg gaactcagct
     2941 gtccccaggc tctggaaggt cagcagctcc tatatgtgac cagagttacg ggactcaact
     3001 gcaccaccaa tcaccccatt aacccaaagg gcctggagtt ggatcccgag ggttccctgc
     3061 accaccagca aaaacgggaa gctccaagcc gcagctctgc ttcctcggga cctcagatcc
     3121 tgaaatgccc ggaggctgag tgtttcaggc tgcgctgtga gctcgggccc ctgcaccaac
     3181 aagagagcca aagtctgcag ttgcatttcc gagtctgggc caagactttc ttgcagcggg
     3241 agcaccagcc atttagcctg cagtgtgagg ctgtgtacaa agccctgaag atgccctacc
     3301 gaatcctgcc tcggcagctg ccccaaaaag agcgtcaggt ggccacagct gtgcaatgga
     3361 ccaaggcaga aggcagctat ggcgtcccac tgtggatcat catcctagcc atcctgtttg
     3421 gcctcctgct cctaggtcta ctcatctaca tcctctacaa gcttggattc ttcaaacgct
     3481 ccctcccata tggcaccgcc atggaaaaag ctcagctcaa gcctccagcc acctctgatg
     3541 ccgaattcga ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg
     3601 atgacgatga caagcttatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     3661 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     3721 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     3781 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     3841 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     3901 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     3961 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     4021 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     4081 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     4141 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     4201 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     4261 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     4321 acaagtgaat tctcgacctc gagacaaatg gcagtattca tccacaattt taaaagaaaa
     4381 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     4441 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     4501 gacagcagag atccactttg gccgcggctc gagggggttg gggttgcgcc ttttccaagg
     4561 cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg
     4621 cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc
     4681 cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc
     4741 cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct
     4801 cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa
     4861 tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc
     4921 ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa
     4981 gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc
     5041 ccagggggat ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
     5101 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     5161 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     5221 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     5281 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     5341 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     5401 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     5461 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     5521 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     5581 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     5641 catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag
     5701 gagcgcacga ccccatgcat cggtaccttt aagaccaatg acttacaagg cagctgtaga
     5761 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     5821 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     5881 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5941 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6001 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     6061 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     6121 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6181 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     6241 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6301 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     6361 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     6421 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     6481 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     6541 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     6601 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6661 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6721 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6781 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6841 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6901 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6961 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7021 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     7081 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7141 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7201 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7261 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7321 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7381 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7441 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7501 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7561 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7621 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7681 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7741 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7801 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7861 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7921 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7981 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8041 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8101 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8161 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8221 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8281 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8341 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8401 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8461 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8521 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8581 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8641 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8701 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8761 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8821 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8881 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8941 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     9001 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     9061 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     9121 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     9181 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     9241 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     9301 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     9361 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     9421 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     9481 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
     9541 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     9601 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     9661 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     9721 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     9781 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     9841 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     9901 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     9961 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
    10021 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
    10081 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
    10141 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
    10201 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
    10261 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
    10321 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
    10381 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
    10441 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
    10501 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
    10561 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
    10621 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
    10681 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
    10741 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
    10801 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
    10861 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
    10921 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
    10981 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
    11041 cgattagtga acggatctcg acggtatcga tcacgagact agcctcgagc ggccgccccc
    11101 ttcaccgagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt
    11161 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
    11221 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
    11281 tatca
//
