LOCUS       V047220                11285 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047220
VERSION     V047220
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5448
                     /label="ITGA5(NM_002205)"
                     /note="ITGA5(NM_002205)"
                     /gene="ITGA5"
     CDS             5488..6204
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived
                     from GFP (Lam et al., 2012)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKL
                     TLKFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFK
                     DDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNG
                     IKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMV
                     LLEFVTAAGITHGMDELYK"
                     /label="Clover"
     promoter        6255..6466
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6479..6747
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6774..7373
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7380..7968
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8042..8275
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8347..8468
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8487..8622
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8660..8676)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8684..8700
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8708..8738)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8753..8774
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9062..9650)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9821..10681)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10682..10786)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11260..11276
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggggagc cggacgccag agtcccctct ccacgccgtg
     2341 cagctgcgct ggggcccccg gcgccgaccc ccgctgctgc cgctgctgtt gctgctgctg
     2401 ccgccgccac ccagggtcgg gggcttcaac ttagacgcgg aggccccagc agtactctcg
     2461 gggcccccgg gctccttctt cggattctca gtggagtttt accggccggg aacagacggg
     2521 gtcagtgtgc tggtgggagc acccaaggct aataccagcc agccaggagt gctgcagggt
     2581 ggtgctgtct acctctgtcc ttggggtgcc agccccacac agtgcacccc cattgaattt
     2641 gacagcaaag gctctcggct cctggagtcc tcactgtcca gctcagaggg agaggagcct
     2701 gtggagtaca agtccttgca gtggttcggg gcaacagttc gagcccatgg ctcctccatc
     2761 ttggcatgcg ctccactgta cagctggcgc acagagaagg agccactgag cgaccccgtg
     2821 ggcacctgct acctctccac agataacttc acccgaattc tggagtatgc accctgccgc
     2881 tcagatttca gctgggcagc aggacagggt tactgccaag gaggcttcag tgccgagttc
     2941 accaagactg gccgtgtggt tttaggtgga ccaggaagct atttctggca aggccagatc
     3001 ctgtctgcca ctcaggagca gattgcagaa tcttattacc ccgagtacct gatcaacctg
     3061 gttcaggggc agctgcagac tcgccaggcc agttccatct atgatgacag ctacctagga
     3121 tactctgtgg ctgttggtga attcagtggt gatgacacag aagactttgt tgctggtgtg
     3181 cccaaaggga acctcactta cggctatgtc accatcctta atggctcaga cattcgatcc
     3241 ctctacaact tctcagggga acagatggcc tcctactttg gctatgcagt ggccgccaca
     3301 gacgtcaatg gggacgggct ggatgacttg ctggtggggg cacccctgct catggatcgg
     3361 acccctgacg ggcggcctca ggaggtgggc agggtctacg tctacctgca gcacccagcc
     3421 ggcatagagc ccacgcccac ccttaccctc actggccatg atgagtttgg ccgatttggc
     3481 agctccttga cccccctggg ggacctggac caggatggct acaatgatgt ggccatcggg
     3541 gctccctttg gtggggagac ccagcaggga gtagtgtttg tatttcctgg gggcccagga
     3601 gggctgggct ctaagccttc ccaggttctg cagcccctgt gggcagccag ccacacccca
     3661 gacttctttg gctctgccct tcgaggaggc cgagacctgg atggcaatgg atatcctgat
     3721 ctgattgtgg ggtcctttgg tgtggacaag gctgtggtat acaggggccg ccccatcgtg
     3781 tccgctagtg cctccctcac catcttcccc gccatgttca acccagagga gcggagctgc
     3841 agcttagagg ggaaccctgt ggcctgcatc aaccttagct tctgcctcaa tgcttctgga
     3901 aaacacgttg ctgactccat tggtttcaca gtggaacttc agctggactg gcagaagcag
     3961 aagggagggg tacggcgggc actgttcctg gcctccaggc aggcaaccct gacccagacc
     4021 ctgctcatcc agaatggggc tcgagaggat tgcagagaga tgaagatcta cctcaggaac
     4081 gagtcagaat ttcgagacaa actctcgccg attcacatcg ctctcaactt ctccttggac
     4141 ccccaagccc cagtggacag ccacggcctc aggccagccc tacattatca gagcaagagc
     4201 cggatagagg acaaggctca gatcttgctg gactgtggag aagacaacat ctgtgtgcct
     4261 gacctgcagc tggaagtgtt tggggagcag aaccatgtgt acctgggtga caagaatgcc
     4321 ctgaacctca ctttccatgc ccagaatgtg ggtgagggtg gcgcctatga ggctgagctt
     4381 cgggtcaccg cccctccaga ggctgagtac tcaggactcg tcagacaccc agggaacttc
     4441 tccagcctga gctgtgacta ctttgccgtg aaccagagcc gcctgctggt gtgtgacctg
     4501 ggcaacccca tgaaggcagg agccagtctg tggggtggcc ttcggtttac agtccctcat
     4561 ctccgggaca ctaagaaaac catccagttt gacttccaga tcctcagcaa gaatctcaac
     4621 aactcgcaaa gcgacgtggt ttcctttcgg ctctccgtgg aggctcaggc ccaggtcacc
     4681 ctgaacggtg tctccaagcc tgaggcagtg ctattcccag taagcgactg gcatccccga
     4741 gaccagcctc agaaggagga ggacctggga cctgctgtcc accatgtcta tgagctcatc
     4801 aaccaaggcc ccagctccat tagccagggt gtgctggaac tcagctgtcc ccaggctctg
     4861 gaaggtcagc agctcctata tgtgaccaga gttacgggac tcaactgcac caccaatcac
     4921 cccattaacc caaagggcct ggagttggat cccgagggtt ccctgcacca ccagcaaaaa
     4981 cgggaagctc caagccgcag ctctgcttcc tcgggacctc agatcctgaa atgcccggag
     5041 gctgagtgtt tcaggctgcg ctgtgagctc gggcccctgc accaacaaga gagccaaagt
     5101 ctgcagttgc atttccgagt ctgggccaag actttcttgc agcgggagca ccagccattt
     5161 agcctgcagt gtgaggctgt gtacaaagcc ctgaagatgc cctaccgaat cctgcctcgg
     5221 cagctgcccc aaaaagagcg tcaggtggcc acagctgtgc aatggaccaa ggcagaaggc
     5281 agctatggcg tcccactgtg gatcatcatc ctagccatcc tgtttggcct cctgctccta
     5341 ggtctactca tctacatcct ctacaagctt ggattcttca aacgctccct cccatatggc
     5401 accgccatgg aaaaagctca gctcaagcct ccagccacct ctgatgccgc ggccgcaggt
     5461 ggaggcggat caggaggcgg tggatctatg gtgagcaagg gcgaggagct gttcaccggg
     5521 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtccgc
     5581 ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc
     5641 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cgtggcctgc
     5701 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     5761 ggctacgtcc aggagcgcac catctctttc aaggacgacg gtacctacaa gacccgcgcc
     5821 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     5881 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acttcaacag ccacaacgtc
     5941 tatatcacgg ccgacaagca gaagaacggc atcaaggcta acttcaagat ccgccacaac
     6001 gttgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     6061 ggccccgtgc tgctgcccga caaccactac ctgagccatc agtccgccct gagcaaagac
     6121 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggattaca
     6181 catggcatgg acgagctgta caagtaagga tccgcggccg cgaaggatct gcgatcgctc
     6241 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     6301 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     6361 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     6421 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg
     6481 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt
     6541 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt
     6601 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag
     6661 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc
     6721 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagatgaccg
     6781 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc
     6841 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca
     6901 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     6961 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     7021 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     7081 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     7141 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     7201 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     7261 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     7321 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgaca
     7381 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     7441 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     7501 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     7561 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     7621 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     7681 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     7741 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     7801 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     7861 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     7921 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta
     7981 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     8041 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct
     8101 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8161 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8221 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     8281 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     8341 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8401 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8461 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt
     8521 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     8581 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg
     8641 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8701 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8761 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8821 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8881 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8941 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     9001 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9061 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9481 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     9541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9661 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9721 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9781 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9841 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9901 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9961 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    10021 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    10081 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    10141 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10201 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10261 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10321 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10381 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10441 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10501 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10561 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10621 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10681 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10741 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10801 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
    10861 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
    10921 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
    10981 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
    11041 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
    11101 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    11161 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    11221 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca
    11281 agctg
//
