LOCUS       V034862                10190 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034862
VERSION     V034862
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4191
                     /label="IRAK2(NM_001570)"
                     /note="IRAK2(NM_001570)"
                     /gene="IRAK2"
     CDS             4198..4224
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4228..4254
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4258..4284
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4349..4560
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4573..4841
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4870..4879
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4900..5562
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5632..5685
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5686..6285
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6286..6873
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6947..7180
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7252..7373
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7392..7527
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7565..7581)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7589..7605
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7613..7643)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7658..7679
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7967..8555)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8726..9586)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9587..9691)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10165..10181
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cctgctacat ctaccagctg
     2341 ccctcctggg tgctggacga cctgtgccgc aacatggacg cgctcagcga gtgggactgg
     2401 atggagttcg cctcctacgt gatcacagac ctgacccagc tgcggaagat caagtccatg
     2461 gagcgggtgc agggtgtgag catcacgcgg gagctgctgt ggtggtgggg catgcggcag
     2521 gccaccgtcc agcaacttgt ggacctcctg tgccgcctgg agctctaccg ggctgcccag
     2581 atcatcctga actggaaacc ggctcctgaa atcaggtgtc ccattccagc cttccctgac
     2641 tctgtgaagc cagaaaagcc tttggcagct tctgtaagaa aggctgagga tgaacaggaa
     2701 gaggggcagc ctgtgaggat ggccaccttt ccaggcccag ggtcctctcc agccagagcc
     2761 caccagccgg cctttctcca gcctcctgaa gaagatgccc ctcattcctt gagaagcgac
     2821 ctccccactt cgtctgattc aaaggacttc agcacctcca ttcctaagca ggaaaaactt
     2881 ttgagcttgg ctggagacag ccttttctgg agtgaggcag acgtggtcca ggcaaccgat
     2941 gacttcaatc aaaaccgcaa aatcagccag gggacctttg ctgacgtcta cagagggcac
     3001 aggcacggga agccattcgt cttcaagaag ctcagagaga cagcctgttc aagtccagga
     3061 tcaatcgaaa gattcttcca ggcagagttg cagatttgtc ttagatgctg ccaccccaat
     3121 gtcttacctg tgctgggctt ctgtgctgca agacagtttc acagcttcat ctacccctac
     3181 atggcaaatg gttccctaca ggacagactg cagggtcagg gtggctcgga ccccctcccc
     3241 tggccccagc gtgtcagcat ctgctcaggg ctgctctgtg ccgtcgagta cctgcatggt
     3301 ctggagatca tccacagcaa cgtcaagagc tctaatgtct tgctggacca aaatctcacc
     3361 cccaaacttg ctcacccaat ggctcatctg tgtcctgtca acaaaaggtc aaaatacacc
     3421 atgatgaaga ctcacctgct ccggacgtca gccgcgtatc tgccagagga tttcatccgg
     3481 gtggggcagc tgacaaagcg agtggacatc ttcagctgtg gaatagtgtt ggccgaggtc
     3541 ctcacgggca tccctgcaat ggataacaac cgaagcccgg tttacctgaa ggacttactc
     3601 ctcagtgata ttccaagcag caccgcctcg ctctgctcca ggaagacggg cgtggagaac
     3661 gtgatggcaa aggagatctg ccagaagtac ctggagaagg gcgcagggag gcttccggag
     3721 gactgcgccg aggccctggc cacggctgcc tgcctgtgcc tgcggaggcg taacaccagc
     3781 ctgcaggagg tgtgtggctc tgtggctgct gtggaagagc ggctccgagg tcgggagacg
     3841 ttgctccctt ggagtgggct ttctgagggt acaggctctt cttccaacac cccagaggaa
     3901 acagacgacg ttgacaattc cagccttgat gcctcctcct ccatgagtgt ggcaccctgg
     3961 gcaggggctg ccaccccact tctccccaca gagaatgggg aaggaaggct gcgggtcatc
     4021 gtgggaaggg aggctgactc ctcctctgag gcctgtgttg gcctggagcc tccccaggat
     4081 gttacagaaa cttcgtggca aattgagatc aatgaggcca aaaggaaact gatggagaat
     4141 attctgctct acaaagagga aaaagtggac agcattgagc tctttggccc cctcgagtac
     4201 ccatacgacg tacctgatta tgcaacttat ccttatgatg tacctgatta tgctagctat
     4261 ccttatgatg tcccagacta cgcataggga tccctgcgat atccagcgcg gccgcgaagg
     4321 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     4381 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     4441 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     4501 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     4561 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     4621 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     4681 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     4741 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     4801 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     4861 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     4921 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     4981 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     5041 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     5101 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     5161 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     5221 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     5281 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     5341 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     5401 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     5461 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     5521 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     5581 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     5641 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     5701 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     5761 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     5821 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     5881 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     5941 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6001 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6061 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6121 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6181 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6241 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     6301 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6361 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     6421 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     6481 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     6541 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     6601 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     6661 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     6721 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     6781 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctccgcct tcgccctcag
     6841 acgagtcgga tctccctttg gccgcctccc cgcctggtac ctttaagacc aatgacttac
     6901 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     6961 cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     7021 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7081 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7141 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     7201 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     7261 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7321 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7381 tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7441 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7501 agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat
     7561 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7621 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7681 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7741 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7801 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7861 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7921 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7981 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8041 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8101 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8161 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8221 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8281 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8341 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8401 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8461 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8521 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8581 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8641 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8701 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8761 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8821 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     8881 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     8941 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9001 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9061 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9121 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9181 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9241 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9301 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9361 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9421 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9481 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9541 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9601 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9661 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     9721 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     9781 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     9841 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     9901 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     9961 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
    10021 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
    10081 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
    10141 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
