LOCUS       V040426                12255 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040426
VERSION     V040426
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..6442
                     /label="INSR(NM_000208)"
                     /note="INSR(NM_000208)"
                     /gene="INSR"
     CDS             6464..7183
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     promoter        7225..7436
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7449..7717
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             7744..8343
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8350..8938
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9012..9245
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9317..9438
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9457..9592
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9630..9646)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9654..9670
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9678..9708)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9723..9744
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10032..10620)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10791..11651)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11652..11756)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12230..12246
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg ccaccggggg ccggcggggg gcggcggccg cgccgctgct
     2341 ggtggcggtg gccgcgctgc tactgggcgc cgcgggccac ctgtaccccg gagaggtgtg
     2401 tcccggcatg gatatccgga acaacctcac taggttgcat gagctggaga attgctctgt
     2461 catcgaagga cacttgcaga tactcttgat gttcaaaacg aggcccgaag atttccgaga
     2521 cctcagtttc cccaaactca tcatgatcac tgattacttg ctgctcttcc gggtctatgg
     2581 gctcgagagc ctgaaggacc tgttccccaa cctcacggtc atccggggat cacgactgtt
     2641 ctttaactac gcgctggtca tcttcgagat ggttcacctc aaggaactcg gcctctacaa
     2701 cctgatgaac atcacccggg gttctgtccg catcgagaag aacaatgagc tctgttactt
     2761 ggccactatc gactggtccc gtatcctgga ttccgtggag gataattaca tcgtgttgaa
     2821 caaagatgac aacgaggagt gtggagacat ctgtccgggt accgcgaagg gcaagaccaa
     2881 ctgccccgcc accgtcatca acgggcagtt tgtcgaacga tgttggactc atagtcactg
     2941 ccagaaagtt tgcccgacca tctgtaagtc acacggctgc accgccgaag gcctctgttg
     3001 ccacagcgag tgcctgggca actgttctca gcccgacgac cccaccaagt gcgtggcctg
     3061 ccgcaacttc tacctggacg gcaggtgtgt ggagacctgc ccgcccccgt actaccactt
     3121 ccaggactgg cgctgtgtga acttcagctt ctgccaggac ctgcaccaca aatgcaagaa
     3181 ctcgcggagg cagggctgcc accagtacgt cattcacaac aacaagtgca tccctgagtg
     3241 tccctccggg tacacgatga attccagcaa cttgctgtgc accccatgcc tgggtccctg
     3301 tcccaaggtg tgccacctcc tagaaggcga gaagaccatc gactcggtga cgtctgccca
     3361 ggagctccga ggatgcaccg tcatcaacgg gagtctgatc atcaacattc gaggaggcaa
     3421 caatctggca gctgagctag aagccaacct cggcctcatt gaagaaattt cagggtatct
     3481 aaaaatccgc cgatcctacg ctctggtgtc actttccttc ttccggaagt tacgtctgat
     3541 tcgaggagag accttggaaa ttgggaacta ctccttctat gccttggaca accagaacct
     3601 aaggcagctc tgggactgga gcaaacacaa cctcaccatc actcagggga aactcttctt
     3661 ccactataac cccaaactct gcttgtcaga aatccacaag atggaagaag tttcaggaac
     3721 caaggggcgc caggagagaa acgacattgc cctgaagacc aatggggacc aggcatcctg
     3781 tgaaaatgag ttacttaaat tttcttacat tcggacatct tttgacaaga tcttgctgag
     3841 atgggagccg tactggcccc ccgacttccg agacctcttg gggttcatgc tgttctacaa
     3901 agaggcccct tatcagaatg tgacggagtt cgacgggcag gatgcgtgtg gttccaacag
     3961 ttggacggtg gtagacattg acccacccct gaggtccaac gaccccaaat cacagaacca
     4021 cccagggtgg ctgatgcggg gtctcaagcc ctggacccag tatgccatct ttgtgaagac
     4081 cctggtcacc ttttcggatg aacgccggac ctatggggcc aagagtgaca tcatttatgt
     4141 ccagacagat gccaccaacc cctctgtgcc cctggatcca atctcagtgt ctaactcatc
     4201 atcccagatt attctgaagt ggaaaccacc ctccgacccc aatggcaaca tcacccacta
     4261 cctggttttc tgggagaggc aggcggaaga cagtgagctg ttcgagctgg attattgcct
     4321 caaagggctg aagctgccct cgaggacctg gtctccacca ttcgagtctg aagattctca
     4381 gaagcacaac cagagtgagt atgaggattc ggccggcgaa tgctgctcct gtccaaagac
     4441 agactctcag atcctgaagg agctggagga gtcctcgttt aggaagacgt ttgaggatta
     4501 cctgcacaac gtggttttcg tccccagaaa aacctcttca ggcactggtg ccgaggaccc
     4561 taggccatct cggaaacgca ggtcccttgg cgatgttggg aatgtgacgg tggccgtgcc
     4621 cacggtggca gctttcccca acacttcctc gaccagcgtg cccacgagtc cggaggagca
     4681 caggcctttt gagaaggtgg tgaacaagga gtcgctggtc atctccggct tgcgacactt
     4741 cacgggctat cgcatcgagc tgcaggcttg caaccaggac acccctgagg aacggtgcag
     4801 tgtggcagcc tacgtcagtg cgaggaccat gcctgaagcc aaggctgatg acattgttgg
     4861 ccctgtgacg catgaaatct ttgagaacaa cgtcgtccac ttgatgtggc aggagccgaa
     4921 ggagcccaat ggtctgatcg tgctgtatga agtgagttat cggcgatatg gtgatgagga
     4981 gctgcatctc tgcgtctccc gcaagcactt cgctctggaa cggggctgca ggctgcgtgg
     5041 gctgtcaccg gggaactaca gcgtgcgaat ccgggccacc tcccttgcgg gcaacggctc
     5101 ttggacggaa cccacctatt tctacgtgac agactattta gacgtcccgt caaatattgc
     5161 aaaaattatc atcggccccc tcatctttgt ctttctcttc agtgttgtga ttggaagtat
     5221 ttatctattc ctgagaaaga ggcagccaga tgggccgctg ggaccgcttt acgcttcttc
     5281 aaaccctgag tatctcagtg ccagtgatgt gtttccatgc tctgtgtacg tgccggacga
     5341 gtgggaggtg tctcgagaga agatcaccct ccttcgagag ctggggcagg gctccttcgg
     5401 catggtgtat gagggcaatg ccagggacat catcaagggt gaggcagaga cccgcgtggc
     5461 ggtgaagacg gtcaacgagt cagccagtct ccgagagcgg attgagttcc tcaatgaggc
     5521 ctcggtcatg aagggcttca cctgccatca cgtggtgcgc ctcctgggag tggtgtccaa
     5581 gggccagccc acgctggtgg tgatggagct gatggctcac ggagacctga agagctacct
     5641 ccgttctctg cggccagagg ctgagaataa tcctggccgc cctcccccta cccttcaaga
     5701 gatgattcag atggcggcag agattgctga cgggatggcc tacctgaacg ccaagaagtt
     5761 tgtgcatcgg gacctggcag cgagaaactg catggtcgcc catgatttta ctgtcaaaat
     5821 tggagacttt ggaatgacca gagacatcta tgaaacggat tactaccgga aagggggcaa
     5881 gggtctgctc cctgtacggt ggatggcacc ggagtccctg aaggatgggg tcttcaccac
     5941 ttcttctgac atgtggtcct ttggcgtggt cctttgggaa atcaccagct tggcagaaca
     6001 gccttaccaa ggcctgtcta atgaacaggt gttgaaattt gtcatggatg gagggtatct
     6061 ggatcaaccc gacaactgtc cagagagagt cactgacctc atgcgcatgt gctggcaatt
     6121 caaccccaag atgaggccaa ccttcctgga gattgtcaac ctgctcaagg acgacctgca
     6181 ccccagcttt ccagaggtgt cgttcttcca cagcgaggag aacaaggctc ccgagagtga
     6241 ggagctggag atggagtttg aggacatgga gaatgtgccc ctggaccgtt cctcgcactg
     6301 tcagagggag gaggcggggg gccgggatgg agggtcctcg ctgggtttca agcggagcta
     6361 cgaggaacac atcccttaca cacacatgaa cggaggcaag aaaaacgggc ggattctgac
     6421 cttgcctcgg tccaatcctt ccggatccgg aggaggggga agcatggtga gcaagggcga
     6481 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     6541 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     6601 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     6661 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     6721 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     6781 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     6841 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     6901 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     6961 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     7021 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     7081 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     7141 cgccgccggg atcactctcg gcatggacga gctgtacaag taagcggccg cgaaggatct
     7201 gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
     7261 tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg
     7321 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     7381 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga
     7441 agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc
     7501 cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg
     7561 ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga
     7621 gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg
     7681 tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc
     7741 tagatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc
     7801 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg
     7861 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     7921 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     7981 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     8041 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     8101 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     8161 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     8221 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     8281 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     8341 tgagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     8401 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     8461 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     8521 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     8581 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     8641 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     8701 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     8761 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     8821 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     8881 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     8941 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     9001 aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg
     9061 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     9121 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     9181 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     9241 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     9301 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     9361 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     9421 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt
     9481 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9541 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt
     9601 tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     9661 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     9721 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     9781 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     9841 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     9901 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     9961 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    10021 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    10081 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    10141 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    10201 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    10261 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    10321 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    10381 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    10441 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
    10501 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    10561 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    10621 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    10681 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    10741 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    10801 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    10861 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    10921 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    10981 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    11041 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    11101 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    11161 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    11221 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    11281 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    11341 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    11401 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    11461 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    11521 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    11581 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    11641 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    11701 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    11761 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
    11821 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
    11881 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
    11941 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
    12001 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
    12061 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
    12121 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
    12181 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
    12241 gccagtgcca agctg
//
