LOCUS       V034980                11432 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034980
VERSION     V034980
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2291..5044
                     /label="IL6ST(NM_002184)"
                     /note="IL6ST(NM_002184)"
                     /gene="IL6ST"
     CDS             5051..5116
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5160..5371
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5384..5652
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5681..5690
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5711..6373
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6443..6496
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6497..7522
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /note="confers resistance to hygromycin"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /transl_table=1
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E*"
                     /label="HygR"
     misc_feature    7529..8117
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8189..8422
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8494..8615
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8634..8769
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8807..8823)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8831..8847
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8855..8885)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8900..8921
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9209..9797)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9968..10828)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10829..10933)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11407..11423
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagatct
     2281 cgaggccacc atgttgacgt tgcagacttg gctagtgcaa gccttgttta ttttcctcac
     2341 cactgaatct acaggtgaac ttctagatcc atgtggttat atcagtcctg aatctccagt
     2401 tgtacaactt cattctaatt tcactgcagt ttgtgtgcta aaggaaaaat gtatggatta
     2461 ttttcatgta aatgctaatt acattgtctg gaaaacaaac cattttacta ttcctaagga
     2521 gcaatatact atcataaaca gaacagcatc cagtgtcacc tttacagata tagcttcatt
     2581 aaatattcag ctcacttgca acattcttac attcggacag cttgaacaga atgtttatgg
     2641 aatcacaata atttcaggct tgcctccaga aaaacctaaa aatttgagtt gcattgtgaa
     2701 cgaggggaag aaaatgaggt gtgagtggga tggtggaagg gaaacacact tggagacaaa
     2761 cttcacttta aaatctgaat gggcaacaca caagtttgct gattgcaaag caaaacgtga
     2821 cacccccacc tcatgcactg ttgattattc tactgtgtat tttgtcaaca ttgaagtctg
     2881 ggtagaagca gagaatgccc ttgggaaggt tacatcagat catatcaatt ttgatcctgt
     2941 atataaagtg aagcccaatc cgccacataa tttatcagtg atcaactcag aggaactgtc
     3001 tagtatctta aaattgacat ggaccaaccc aagtattaag agtgttataa tactaaaata
     3061 taacattcaa tataggacca aagatgcctc aacttggagc cagattcctc ctgaagacac
     3121 agcatccacc cgatcttcat tcactgtcca agaccttaaa ccttttacag aatatgtgtt
     3181 taggattcgc tgtatgaagg aagatggtaa gggatactgg agtgactgga gtgaagaagc
     3241 aagtgggatc acctatgaag atagaccatc taaagcacca agtttctggt ataaaataga
     3301 tccatcccat actcaaggct acagaactgt acaactcgtg tggaagacat tgcctccttt
     3361 tgaagccaat ggaaaaatct tggattatga agtgactctc acaagatgga aatcacattt
     3421 acaaaattac acagttaatg ccacaaaact gacagtaaat ctcacaaatg atcgctatct
     3481 agcaacccta acagtaagaa atcttgttgg caaatcagat gcagctgttt taactatccc
     3541 tgcctgtgac tttcaagcta ctcaccctgt aatggatctt aaagcattcc ccaaagataa
     3601 catgctttgg gtggaatgga ctactccaag ggaatctgta aagaaatata tacttgagtg
     3661 gtgtgtgtta tcagataaag caccctgtat cacagactgg caacaagaag atggtaccgt
     3721 gcatcgcacc tatttaagag ggaacttagc agagagcaaa tgctatttga taacagttac
     3781 tccagtatat gctgatggac caggaagccc tgaatccata aaggcatacc ttaaacaagc
     3841 tccaccttcc aaaggaccta ctgttcggac aaaaaaagta gggaaaaacg aagctgtctt
     3901 agagtgggac caacttcctg ttgatgttca gaatggattt atcagaaatt atactatatt
     3961 ttatagaacc atcattggaa atgaaactgc tgtgaatgtg gattcttccc acacagaata
     4021 tacattgtcc tctttgacta gtgacacatt gtacatggta cgaatggcag catacacaga
     4081 tgaaggtggg aaggatggtc cagaattcac ttttactacc ccaaagtttg ctcaaggaga
     4141 aattgaagcc atagtcgtgc ctgtttgctt agcattccta ttgacaactc ttctgggagt
     4201 gctgttctgc tttaataagc gagacctaat taaaaaacac atctggccta atgttccaga
     4261 tccttcaaag agtcatattg cccagtggtc acctcacact cctccaaggc acaattttaa
     4321 ttcaaaagat caaatgtatt cagatggcaa tttcactgat gtaagtgttg tggaaataga
     4381 agcaaatgac aaaaagcctt ttccagaaga tctgaaatca ttggacctgt tcaaaaagga
     4441 aaaaattaat actgaaggac acagcagtgg tattgggggg tcttcatgca tgtcatcttc
     4501 taggccaagc atttctagca gtgatgaaaa tgaatcttca caaaacactt cgagcactgt
     4561 ccagtattct accgtggtac acagtggcta cagacaccaa gttccgtcag tccaagtctt
     4621 ctcaagatcc gagtctaccc agcccttgtt agattcagag gagcggccag aagatctaca
     4681 attagtagat catgtagatg gcggtgatgg tattttgccc aggcaacagt acttcaaaca
     4741 gaactgcagt cagcatgaat ccagtccaga tatttcacat tttgaaaggt caaagcaagt
     4801 ttcatcagtc aatgaggaag attttgttag acttaaacag cagatttcag atcatatttc
     4861 acaatcctgt ggatctgggc aaatgaaaat gtttcaggaa gtttctgcag cagatgcttt
     4921 tggtccaggt actgagggac aagtagaaag atttgaaaca gttggcatgg aggctgcgac
     4981 tgatgaaggc atgcctaaaa gttacttacc acagactgta cggcaaggcg gctacatgcc
     5041 tcagggatcc gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
     5101 ggatgacgat gacaagtagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg
     5161 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     5221 gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     5281 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     5341 ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc
     5401 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc
     5461 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag
     5521 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc
     5581 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg
     5641 ccgttacaga tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga
     5701 gagcggcctg cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga
     5761 gttcgagctg gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat
     5821 gaagagcacc aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta
     5881 cggcttctac cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat
     5941 caacaacggc ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca
     6001 cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg
     6061 caccggcttc cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac
     6121 cgtggagcac ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt
     6181 cagcctgcgc gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag
     6241 cgccatccac cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga
     6301 ggagctgcac agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc
     6361 catcgccttc gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg
     6421 acccggctcc accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga
     6481 ggagaatccc ggccctatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct
     6541 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
     6601 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
     6661 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
     6721 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
     6781 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
     6841 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     6901 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     6961 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     7021 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     7081 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     7141 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     7201 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     7261 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
     7321 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
     7381 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     7441 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     7501 tcgtccgagg gcaaaggaat aggtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     7561 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     7621 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     7681 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     7741 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     7801 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     7861 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     7921 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     7981 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     8041 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     8101 ttgggccgcc tccccgcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     8161 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa
     8221 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     8281 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8341 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8401 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     8461 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     8521 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8581 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     8641 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     8701 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     8761 ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc
     8821 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8881 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8941 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     9001 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     9061 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     9121 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     9181 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     9241 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9301 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9361 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9421 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9481 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9541 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9601 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     9661 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9721 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9781 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9841 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9901 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9961 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    10021 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    10081 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    10141 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    10201 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    10261 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10321 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10381 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10441 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10501 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10561 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10621 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10681 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10741 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10801 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10861 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10921 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
    10981 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
    11041 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
    11101 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
    11161 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
    11221 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
    11281 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
    11341 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
    11401 gacgttgtaa aacgacggcc agtgccaagc tg
//
