LOCUS V038545 10502 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038545 VERSION V038545 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..4503 /label="IFI16(NM_001206567)" /note="IFI16(NM_001206567)" /gene="IFI16" CDS 4510..4536 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4540..4566 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4570..4596 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 4661..4872 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4885..5153 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 5182..5191 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 5212..5874 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 5944..5997 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 5998..6597 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6598..7185 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7259..7492 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7564..7685 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7704..7839 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7877..7893) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7901..7917 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7925..7955) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7970..7991 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8279..8867) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9038..9898) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9899..10003) /gene="bla" /label="AmpR promoter" primer_bind 10477..10493 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gaaaaaaata caagaacatt 2341 gttctactaa aaggattaga ggtcatcaat gattatcatt ttagaatggt taagtcctta 2401 ctgagcaacg atttaaaact taatttaaaa atgagagaag agtatgacaa aattcagatt 2461 gctgacttga tggaagaaaa gttccgaggt gatgctggtt tgggcaaact aataaaaatt 2521 ttcgaagata taccaacgct tgaagacctg gctgaaactc ttaaaaaaga aaagttaaaa 2581 gtaaaaggac cagccctatc aagaaagagg aagaaggaag tggatgctac ttcacctgca 2641 ccctccacaa gcagcactgt caaaactgaa ggagcagagg caactcctgg agctcagaac 2701 ccgaaaacag tggccaaatg tcaggtaact cccagaagaa atgttctcca aaaacgccca 2761 gtgatagtga aggtactgag tacaacaaag ccatttgaat atgagacccc agaaatggag 2821 aaaaaaataa tgtttcatgc tacagtggct acacagacac agttcttcca tgtgaaggtt 2881 ttaaacacca gcttgaagga gaaattcaat ggaaagaaaa tcatcatcat atcagattat 2941 ttggaatatg atagtctcct agaggtcaat gaagaatcta ctgtatctga agctggtcct 3001 aaccaaacgt ttgaggttcc aaataaaatc atcaacagag caaaggaaac tctgaagatt 3061 gatattcttc acaaacaagc ttcaggaaat attgtatatg gggtatttat gctacataag 3121 aaaacagtaa atcagaagac cacaatctac gaaattcagg atgatagagg aaaaatggat 3181 gtagtgggga caggacaatg tcacaatatc ccctgtgaag aaggagataa gctccaactt 3241 ttctgctttc gacttagaaa aaagaaccag atgtcaaaac tgatttcaga aatgcatagt 3301 tttatccaga taaagaaaaa aacaaacccg agaaacaatg accccaagag catgaagcta 3361 ccccaggaac agcgtcagct tccatatcct tcagaggcca gcacaacctt ccctgagagc 3421 catcttcgga ctcctcagat gccaccaaca actccatcca gcagtttctt caccaagaaa 3481 agtgaagaca caatctccaa aatgaatgac ttcatgagga tgcagatact gaaggaaggg 3541 agtcattttc caggaccgtt catgaccagc ataggcccag ctgagagcca tccccacact 3601 cctcagatgc ctccatcaac accaagcagc agtttcttaa ccacgaaaag tgaagacaca 3661 atctccaaaa tgaatgactt catgaggatg cagatactga aggaagggag tcattttcca 3721 ggaccgttca tgaccagcat aggcccagct gagagccatc cccacactcc tcagatgcct 3781 ccatcaacac caagcagcag tttcttaacc acgttgaaac caagactgaa gactgaacct 3841 gaagaagttt ccatagaaga cagtgcccag agtgacctca aagaagtgat ggtgctgaac 3901 gcaacagaat catttgtata tgagcccaaa gagcagaaga aaatgtttca tgccacagtg 3961 gcaactgaga atgaagtctt ccgagtgaag gtttttaata ttgacctaaa ggagaagttc 4021 accccaaaga agatcattgc catagcaaat tatgtttgcc gcaatgggtt cctggaggta 4081 tatcctttca cacttgtggc tgatgtgaat gctgaccgaa acatggagat cccaaaagga 4141 ttgattagaa gtgccagcgt aactcctaaa atcaatcagc tttgctcaca aactaaagga 4201 agttttgtga atggggtgtt tgaggtacat aagaaaaatg taaggggtga attcacttat 4261 tatgaaatac aagataatac agggaagatg gaagtggtgg tgcatggacg actgaccaca 4321 atcaactgtg aggaaggaga taaactgaaa ctcacctgct ttgaattggc accgaaaagt 4381 gggaataccg gggagttgag atctgtaatt catagtcaca tcaaggtcat caagaccagg 4441 aaaaacaaga aagacatact caatcctgat tcaagtatgg aaacttcacc agactttttc 4501 ttcctcgagt acccatacga cgtacctgat tatgcaactt atccttatga tgtacctgat 4561 tatgctagct atccttatga tgtcccagac tacgcatagg gatccctgcg atatccagcg 4621 cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc 4681 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg 4741 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg 4801 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc 4861 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct 4921 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc 4981 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt 5041 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg 5101 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg 5161 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg 5221 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc 5281 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc 5341 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc 5401 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc 5461 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc 5521 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac 5581 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc 5641 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc 5701 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc 5761 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc 5821 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc 5881 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct 5941 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg 6001 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc 6061 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc 6121 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc 6181 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc 6241 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc 6301 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc 6361 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc 6421 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag 6481 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac 6541 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat 6601 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 6661 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 6721 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 6781 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 6841 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 6901 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 6961 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc 7021 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 7081 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc 7141 cttcgccctc agacgagtcg gatctccctt tggccgcctc cccgcctggt acctttaaga 7201 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 7261 gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc 7321 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 7381 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 7441 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt 7501 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 7561 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 7621 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 7681 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc 7741 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 7801 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc 7861 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 7921 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 7981 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 8041 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 8101 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 8161 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 8221 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 8281 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 8341 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 8401 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 8461 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 8521 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 8581 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 8641 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 8701 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 8761 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 8821 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 8881 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 8941 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 9001 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 9061 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 9121 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 9181 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 9241 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 9301 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 9361 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 9421 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 9481 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 9541 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 9601 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 9661 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 9721 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 9781 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 9841 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 9901 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 9961 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 10021 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 10081 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 10141 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 10201 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc 10261 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 10321 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 10381 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa 10441 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc 10501 tg //