LOCUS       V037146                10250 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037146
VERSION     V037146
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4678
                     /label="RRM1(NM_001033)"
                     /note="RRM1(NM_001033)"
                     /gene="RRM1"
     CDS             4685..4750
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4793..5004
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5017..5285
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5313..6338
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /note="confers resistance to hygromycin"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /transl_table=1
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E*"
                     /label="HygR"
     misc_feature    6345..6933
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7007..7240
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7312..7433
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7452..7587
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7625..7641)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7649..7665
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7673..7703)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7718..7739
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8027..8615)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8786..9646)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9647..9751)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10225..10241
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcc ggatccgcca ccatgcatgt gatcaagcga gatggccgcc aagaacgagt
     2341 catgtttgac aaaattacat ctcgaatcca gaagctttgt tatggactca atatggattt
     2401 tgttgatcct gctcagatca ccatgaaagt aatccaaggc ttgtacagtg gggtcaccac
     2461 agtggaacta gatactttgg ctgctgaaac agctgcaacc ttgactacta agcaccctga
     2521 ctatgctatc ctggcagcca ggatcgctgt ctctaacttg cacaaagaaa caaagaaagt
     2581 gttcagtgat gtgatggaag acctctataa ctacataaat ccacataatg gcaaacactc
     2641 tcccatggtg gccaagtcaa cattggatat tgttctggcc aataaagatc gcctgaattc
     2701 tgctattatc tatgaccgag atttctctta caattacttc ggctttaaga cgctagagcg
     2761 gtcttatttg ttgaagatca atggaaaagt ggctgaaaga ccacaacata tgttgatgag
     2821 agtatctgtt gggatccaca aagaagacat tgatgcagca attgaaacat ataatcttct
     2881 ttctgagagg tggtttactc atgcttcgcc cactctcttc aatgctggta ccaaccgccc
     2941 acaactttct agctgttttc ttctgagtat gaaagatgac agcattgaag gcatttatga
     3001 cactctaaag caatgtgcat tgatttctaa gtctgctgga ggaattggtg ttgctgtgag
     3061 ttgtattcgg gctactggca gctacattgc tgggactaat ggcaattcca atggccttgt
     3121 accgatgctg agagtatata acaacacagc tcgatatgtg gatcaaggtg ggaacaagcg
     3181 tcctggggca tttgctattt acctggagcc ttggcattta gacatctttg aattccttga
     3241 tttaaagaag aacacaggaa aggaagagca gcgtgccaga gatcttttct ttgctctttg
     3301 gattccggat ctcttcatga aacgagtgga gactaatcag gactggtctt tgatgtgtcc
     3361 aaatgagtgt cctggtctgg atgaggtttg gggagaggaa tttgagaaac tatatgcaag
     3421 ttatgagaaa caaggtcgtg tccgcaaagt tgtaaaagct cagcagcttt ggtatgccat
     3481 cattgagtct cagacggaaa caggcacccc gtatatgctc tacaaagatt cctgtaatcg
     3541 aaagagcaac cagcagaacc tgggaaccat caaatgcagc aacctgtgca cagaaatagt
     3601 ggagtacacc agcaaagatg aggttgctgt ttgtaatttg gcttccctgg ccctgaatat
     3661 gtatgtcaca tcagaacaca catacgactt taagaagttg gctgaagtca ctaaagtcgt
     3721 tgtccgaaac ttgaataaaa ttattgatat aaactactat cctgtaccag aggcatgcct
     3781 atcaaataaa cgccatcgcc ccattggaat tggggtacaa ggtctggcag atgcttttat
     3841 cctgatgaga tacccttttg agagtgcaga agcccagtta ctgaataagc agatctttga
     3901 aactatttat tatggtgctc tggaagccag ctgtgacctt gccaaggagc agggcccata
     3961 cgaaacctat gagggctctc cagttagcaa aggaattctt cagtatgata tgtggaatgt
     4021 tactcctaca gacctatggg actggaaggt tctcaaggag aagattgcaa agtatggtat
     4081 aagaaacagt ttacttattg ccccgatgcc tacagcttcc actgctcaga tcctggggaa
     4141 taatgagtcc attgaacctt acaccagcaa catctatact cgcagagtct tgtcaggaga
     4201 atttcagatt gtaaatcctc acttattgaa agatcttacc gagcggggcc tatggcatga
     4261 agagatgaaa aaccagatta ttgcatgcaa tggctctatt cagagcatac cagaaattcc
     4321 tgatgacctg aagcaacttt ataaaactgt gtgggaaatc tctcagaaaa ctgttctcaa
     4381 gatggcagct gagagaggtg ctttcattga tcaaagccaa tctttgaaca tccacattgc
     4441 tgagcctaac tatggcaaac tcactagtat gcacttctac ggctggaagc agggtttgaa
     4501 gactgggatg tattatttaa ggacaagacc agcggctaat ccaatccagt tcactctaaa
     4561 taaggagaag ctaaaagata aagaaaaggt atcaaaagag gaagaagaga aggagaggaa
     4621 cacagcagcc atggtgtgct ctttggagaa tagagatgaa tgtctgatgt gtggatccct
     4681 cgaggactac aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga
     4741 cgatgacaag tagcggccgc aaggatctgc gatcgctccg gtgcccgtca gtgggcagag
     4801 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
     4861 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     4921 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     4981 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
     5041 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
     5101 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
     5161 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
     5221 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
     5281 agatccaagc tgtgaccggc gcctacgtcg acatgaaaaa gcctgaactc accgcgacgt
     5341 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     5401 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     5461 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
     5521 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
     5581 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
     5641 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
     5701 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
     5761 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
     5821 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
     5881 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
     5941 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
     6001 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
     6061 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
     6121 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     6181 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     6241 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     6301 accgacgccc cagcactcgt ccgagggcaa aggaataggt cgacaatcaa cctctggatt
     6361 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     6421 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     6481 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     6541 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     6601 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     6661 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     6721 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     6781 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     6841 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     6901 cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac
     6961 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     7021 cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     7081 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7141 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7201 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     7261 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     7321 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7381 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7441 tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7501 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7561 agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat
     7621 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7681 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7741 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7801 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7861 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7921 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7981 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8041 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8101 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8161 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8221 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8281 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8341 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8401 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8461 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8521 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8581 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8641 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8701 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8761 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8821 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8881 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     8941 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9001 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9061 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9121 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9181 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9241 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9301 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9361 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9421 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9481 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9541 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9601 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9661 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9721 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     9781 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     9841 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     9901 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     9961 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
    10021 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
    10081 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
    10141 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
    10201 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
