LOCUS V020711 9128 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V020711 VERSION V020711 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2294..3595 /label="HDAC3(NM_001355039)" /note="HDAC3(NM_001355039)" /gene="HDAC3" CDS 3596..3622 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 3671..3882 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3895..4163 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4191..5216 /codon_start=1 /gene="aph(4)-Ia" /note="confers resistance to hygromycin" /product="aminoglycoside phosphotransferase from E. coli" /transl_table=1 /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E*" /label="HygR" misc_feature 5223..5811 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5885..6118 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6190..6311 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6330..6465 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6503..6519) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6527..6543 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6551..6581) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6596..6617 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6905..7493) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7664..8524) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8525..8629) /gene="bla" /label="AmpR promoter" primer_bind 9103..9119 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcc accatggcca agaccgtggc ctatttctac gaccccgacg tgggcaactt 2341 ccactacgga gctggacacc ctatgaagcc ccatcgcctg gcattgaccc atagcctggt 2401 cctgcattac ggtctctata agaagatgat cgtcttcaag ccataccagg cctcccaaca 2461 tgacatgtgc cgcttccact ccgaggacta cattgacttc ctgcagagag tcagccccac 2521 caatatgcaa ggcttcacca agagtcttaa tgccttcaac gtaggcgatg actgcccagt 2581 gtttcccggg ctctttgagt tctgctcgcg ttacacaggc gcatctctgc aaggagcaac 2641 ccagctgaac aacaagatct gtgatattgc cattaactgg gctggtggtc tgcaccatgc 2701 caagaagttt gaggcctctg gcttctgcta tgtcaacgac attgtgattg gcatcctgga 2761 gctgctcaag taccaccctc gggtgctcta cattgacatt gacatccacc atggtgacgg 2821 ggttcaagaa gctttctacc tcactgaccg ggtcatgacg gtgtccttcc acaaatacgg 2881 aaattacttc ttccctggca caggtgacat gtatgaagtc ggggcagaga gtggccgcta 2941 ctactgtctg aacgtgcccc tgcgggatgg cattgatgac cagagttaca agcacctttt 3001 ccagccggtt atcaaccagg tagtggactt ctaccaaccc acgtgcattg tgctccagtg 3061 tggagctgac tctctgggct gtgatcgatt gggctgcttt aacctcagca tccgagggca 3121 tggggaatgc gttgaatatg tcaagagctt caatatccct ctactcgtgc tgggtggtgg 3181 tggttatact gtccgaaatg ttgcccgctg ctggacatat gagacatcgc tgctggtaga 3241 agaggccatt agtgaggagc ttccctatag tgaatacttc gagtactttg ccccagactt 3301 cacacttcat ccagatgtca gcacccgcat cgagaatcag aactcacgcc agtatctgga 3361 ccagatccgc cagacaatct ttgaaaacct gaagatgctg aaccatgcac ctagtgtcca 3421 gattcatgac gtgcctgcag acctcctgac ctatgacagg actgatgagg ctgatgcaga 3481 ggagaggggt cctgaggaga actatagcag gtctgggagc agggacttca gcctactttc 3541 aataggcttg ctggggaggt ttaaaaggga aaaagtaaat gggaaggtat tagtttaccc 3601 atacgacgta ccagattacg cttaggatcc gcggccgcaa ggatctgcga tcgctccggt 3661 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 3721 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 3781 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 3841 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 3901 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 3961 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 4021 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 4081 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 4141 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgtcgac atgaaaaagc 4201 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg 4261 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc 4321 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt 4381 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca 4441 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc 4501 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg 4561 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat 4621 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa 4681 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt 4741 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg 4801 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg 4861 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc 4921 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg 4981 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg 5041 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg 5101 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag 5161 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaataggtcg 5221 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 5281 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 5341 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 5401 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 5461 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 5521 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 5581 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc 5641 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 5701 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 5761 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct 5821 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg 5881 ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg 5941 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 6001 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 6061 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 6121 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag 6181 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 6241 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 6301 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc 6361 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 6421 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag 6481 acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 6541 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 6601 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 6661 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 6721 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 6781 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 6841 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 6901 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 6961 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 7021 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 7081 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 7141 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 7201 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 7261 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 7321 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 7381 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 7441 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 7501 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 7561 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 7621 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 7681 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 7741 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 7801 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 7861 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 7921 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 7981 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 8041 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 8101 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 8161 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 8221 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 8281 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 8341 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 8401 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 8461 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 8521 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 8581 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 8641 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 8701 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac 8761 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 8821 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat 8881 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa 8941 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 9001 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc 9061 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 9121 ccaagctg //