LOCUS V045886 9184 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V045886 VERSION V045886 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2290..3573 /label="ELK1(NM_001114123)" /note="ELK1(NM_001114123)" /gene="ELK1" regulatory 3580..3589 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 3589..3678 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" promoter 3727..3938 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3951..4219 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4247..5272 /codon_start=1 /gene="aph(4)-Ia" /note="confers resistance to hygromycin" /product="aminoglycoside phosphotransferase from E. coli" /transl_table=1 /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E*" /label="HygR" misc_feature 5279..5867 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5941..6174 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6246..6367 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6386..6521 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6559..6575) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6583..6599 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6607..6637) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6652..6673 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6961..7549) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7720..8580) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8581..8685) /gene="bla" /label="AmpR promoter" primer_bind 9159..9175 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagccacca tggacccatc tgtgacgctg tggcagtttc tgctgcagct gctgagagag 2341 caaggcaatg gccacatcat ctcctggact tcacgggatg gtggtgaatt caagctggtg 2401 gatgcagagg aggtggcccg gctgtggggg ctacgcaaga acaagaccaa catgaattac 2461 gacaagctca gccgggcctt gcggtactac tatgacaaga acatcatccg caaggtgagc 2521 ggccagaagt tcgtctacaa gtttgtgtcc taccctgagg tcgcagggtg ctccactgag 2581 gactgcccgc cccagccaga ggtgtctgtt acctccacca tgccaaatgt ggcccctgct 2641 gctatacatg ccgccccagg ggacactgtc tctggaaagc caggcacacc caagggtgca 2701 ggaatggcag gcccaggcgg tttggcacgc agcagccgga acgagtacat gcgctcgggc 2761 ctctattcca ccttcaccat ccagtctctg cagccgcagc caccccctca tcctcggcct 2821 gctgtggtgc tccccagtgc agctcctgca ggggcagcag cgcccccctc ggggagcagg 2881 agcaccagtc caagcccctt ggaggcctgt ctggaggctg aagaggccgg cttgcctctg 2941 caggtcatcc tgaccccgcc cgaggcccca aacctgaaat cggaagagct taatgtggag 3001 ccgggtttgg gccgggcttt gcccccagaa gtgaaagtag aagggcccaa ggaagagttg 3061 gaagttgcgg gggagagagg gtttgtgcca gaaaccacca aggccgagcc agaagtccct 3121 ccacaggagg gcgtgccagc ccggctgccc gcggttgtta tggacaccgc agggcaggcg 3181 ggcggccatg cggcttccag ccctgagatc tcccagccgc agaagggccg gaagccccgg 3241 gacctagagc ttccactcag cccgagcctg ctaggtgggc cgggacccga acggacccca 3301 ggatcgggaa gtggctccgg cctccaggct ccggggccgg cgctgacccc atccctgctt 3361 cctacgcata cattgacccc ggtgctgctg acacccagct cgctgcctcc tagcattcac 3421 ttctggagca ccctgagtcc cattgcgccc cgtagcccgg ccaagctctc cttccagttt 3481 ccatccagtg gcagcgccca ggtgcacatc ccttctatca gcgtggatgg cctctcgacc 3541 cccgtggtgc tctccccagg gccccagaag ccactcgagg ccaccatgga acaaaaacta 3601 atatcagagg aagatttgga acagaaacta atcagtgaag aagatttaga gcaaaagtta 3661 atttccgagg aggacttata ggatccgcgg ccgcaaggat ctgcgatcgc tccggtgccc 3721 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca 3781 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact 3841 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga 3901 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca 3961 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg 4021 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa 4081 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc 4141 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg 4201 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gtcgacatga aaaagcctga 4261 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct 4321 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg 4381 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg 4441 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga 4501 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga 4561 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc 4621 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac 4681 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt 4741 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc 4801 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct 4861 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc 4921 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca 4981 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta 5041 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga 5101 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg 5161 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact 5221 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat aggtcgacaa 5281 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 5341 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 5401 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 5461 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 5521 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 5581 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 5641 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc 5701 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 5761 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 5821 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa 5881 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 5941 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc 6001 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 6061 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 6121 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 6181 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 6241 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 6301 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 6361 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc 6421 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 6481 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg 6541 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 6601 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 6661 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 6721 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 6781 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 6841 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 6901 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 6961 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 7021 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 7081 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 7141 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 7201 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 7261 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 7321 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 7381 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 7441 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 7501 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 7561 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 7621 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 7681 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 7741 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 7801 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 7861 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 7921 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 7981 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 8041 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 8101 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 8161 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 8221 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 8281 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 8341 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 8401 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 8461 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 8521 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 8581 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 8641 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 8701 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 8761 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 8821 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 8881 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 8941 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 9001 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 9061 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 9121 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 9181 gctg //