LOCUS       V037083                10480 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037083
VERSION     V037083
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5334
                     /label="HDAC9(NM_058176)"
                     /note="HDAC9(NM_058176)"
                     /gene="HDAC9"
     CDS             5341..5406
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5450..5661
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5674..5942
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5969..6568
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6575..7163
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7237..7470
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7542..7663
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7682..7817
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7855..7871)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7879..7895
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7903..7933)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7948..7969
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8257..8845)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9016..9876)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9877..9981)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10455..10471
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgcacagt atgatcagct cagtggatgt gaagtcagaa
     2341 gttcctgtgg gcctggagcc catctcacct ttagacctaa ggacagacct caggatgatg
     2401 atgcccgtgg tggaccctgt tgtccgtgag aagcaattgc agcaggaatt acttcttatc
     2461 cagcagcagc aacaaatcca gaagcagctt ctgatagcag agtttcagaa acagcatgag
     2521 aacttgacac ggcagcacca ggctcagctt caggagcata tcaaggaact tctagccata
     2581 aaacagcaac aagaactcct agaaaaggag cagaaactgg agcagcagag gcaagaacag
     2641 gaagtagaga ggcatcgcag agaacagcag cttcctcctc tcagaggcaa agatagagga
     2701 cgagaaaggg cagtggcaag tacagaagta aagcagaagc ttcaagagtt cctactgagt
     2761 aaatcagcaa cgaaagacac tccaactaat ggaaaaaatc attccgtgag ccgccatccc
     2821 aagctctggt acacggctgc ccaccacaca tcattggatc aaagctctcc accccttagt
     2881 ggaacatctc catcctacaa gtacacatta ccaggagcac aagatgcaaa ggatgatttc
     2941 ccccttcgaa aaactgcctc tgagcccaac ttgaaggtgc ggtccaggtt aaaacagaaa
     3001 gtggcagaga ggagaagcag ccccttactc aggcggaagg atggaaatgt tgtcacttca
     3061 ttcaagaagc gaatgtttga ggtgacagaa tcctcagtca gtagcagttc tccaggctct
     3121 ggtcccagtt caccaaacaa tgggccaact ggaagtgtta ctgaaaatga gacttcggtt
     3181 ttgcccccta cccctcatgc cgagcaaatg gtttcacagc aacgcattct aattcatgaa
     3241 gattccatga acctgctaag tctttatacc tctccttctt tgcccaacat taccttgggg
     3301 cttcccgcag tgccatccca gctcaatgct tcgaattcac tcaaagaaaa gcagaagtgt
     3361 gagacgcaga cgcttaggca aggtgttcct ctgcctgggc agtatggagg cagcatcccg
     3421 gcatcttcca gccaccctca tgttacttta gagggaaagc cacccaacag cagccaccag
     3481 gctctcctgc agcatttatt attgaaagaa caaatgcgac agcaaaagct tcttgtagct
     3541 ggtggagttc ccttacatcc tcagtctccc ttggcaacaa aagagagaat ttcacctggc
     3601 attagaggta cccacaaatt gccccgtcac agacccctga accgaaccca gtctgcacct
     3661 ttgcctcaga gcacgttggc tcagctggtc attcaacagc aacaccagca attcttggag
     3721 aagcagaagc aataccagca gcagatccac atgaacaaac tgctttcgaa atctattgaa
     3781 caactgaagc aaccaggcag tcaccttgag gaagcagagg aagagcttca gggggaccag
     3841 gcgatgcagg aagacagagc gccctctagt ggcaacagca ctaggagcga cagcagtgct
     3901 tgtgtggatg acacactggg acaagttggg gctgtgaagg tcaaggagga accagtggac
     3961 agtgatgaag atgctcagat ccaggaaatg gaatctgggg agcaggctgc ttttatgcaa
     4021 cagcctttcc tggaacccac gcacacacgt gcgctctctg tgcgccaagc tccgctggct
     4081 gcggttggca tggatggatt agagaaacac cgtctcgtct ccaggactca ctcttcccct
     4141 gctgcctctg ttttacctca cccagcaatg gaccgccccc tccagcctgg ctctgcaact
     4201 ggaattgcct atgacccctt gatgctgaaa caccagtgcg tttgtggcaa ttccaccacc
     4261 caccctgagc atgctggacg aatacagagt atctggtcac gactgcaaga aactgggctg
     4321 ctaaataaat gtgagcgaat tcaaggtcga aaagccagcc tggaggaaat acagcttgtt
     4381 cattctgaac atcactcact gttgtatggc accaaccccc tggacggaca gaagctggac
     4441 cccaggatac tcctaggtga tgactctcaa aagttttttt cctcattacc ttgtggtgga
     4501 cttggggtgg acagtgacac catttggaat gagctacact cgtccggtgc tgcacgcatg
     4561 gctgttggct gtgtcatcga gctggcttcc aaagtggcct caggagagct gaagaatggg
     4621 tttgctgttg tgaggccccc tggccatcac gctgaagaat ccacagccat ggggttctgc
     4681 ttttttaatt cagttgcaat taccgccaaa tacttgagag accaactaaa tataagcaag
     4741 atattgattg tagatctgga tgttcaccat ggaaacggta cccagcaggc cttttatgct
     4801 gaccccagca tcctgtacat ttcactccat cgctatgatg aagggaactt tttccctggc
     4861 agtggagccc caaatgaggt tggaacaggc cttggagaag ggtacaatat aaatattgcc
     4921 tggacaggtg gccttgatcc tcccatggga gatgttgagt accttgaagc attcaggacc
     4981 atcgtgaagc ctgtggccaa agagtttgat ccagacatgg tcttagtatc tgctggattt
     5041 gatgcattgg aaggccacac ccctcctcta ggagggtaca aagtgacggc aaaatgtttt
     5101 ggtcatttga cgaagcaatt gatgacattg gctgatggac gtgtggtgtt ggctctagaa
     5161 ggaggacatg atctcacagc catctgtgat gcatcagaag cctgtgtaaa tgcccttcta
     5221 ggaaatgagc tggagccact tgcagaagat attctccacc aaagcccgaa tatgaatgct
     5281 gttatttctt tacagaagat cattgaaatt caaagtatgt ctttaaagtt ctctggatcc
     5341 gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat
     5401 gacaagtagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     5461 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     5521 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     5581 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     5641 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     5701 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     5761 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     5821 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     5881 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     5941 tccaagctgt gaccggcgcc tacgctagat gaccgagtac aagcccacgg tgcgcctcgc
     6001 cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     6061 cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga
     6121 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     6181 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     6241 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     6301 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     6361 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     6421 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     6481 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     6541 gtgcatgacc cgcaagcccg gtgcctgagt cgacaatcaa cctctggatt acaaaatttg
     6601 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     6661 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     6721 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     6781 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     6841 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     6901 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     6961 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     7021 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     7081 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     7141 ctccctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg
     7201 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7261 gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7321 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7381 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7441 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     7501 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     7561 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7621 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     7681 tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7741 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7801 aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata
     7861 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7921 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7981 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8041 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8101 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8161 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8221 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8281 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8341 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8401 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8461 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8521 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8581 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8641 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     8701 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8761 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8821 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8881 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8941 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9001 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9061 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9121 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9181 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9241 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9301 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9361 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9421 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9481 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9541 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9601 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9661 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9721 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9781 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9841 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9901 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9961 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    10021 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    10081 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    10141 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    10201 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    10261 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    10321 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    10381 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    10441 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
