LOCUS       V030093                10681 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030093
VERSION     V030093
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4707
                     /label="H6pd(NM_173371)"
                     /note="H6pd(NM_173371)"
                     /gene="H6pd"
     CDS             4714..4779
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4823..5034
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5047..5315
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5344..5353
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5374..6036
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6106..6159
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6160..6759
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6760..7348
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7422..7655
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7727..7848
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7888..8023
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8056..8072)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8080..8096
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8104..8134)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8149..8170
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8458..9046)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9217..10077)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10078..10182)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10656..10672
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga agcacacagg catttggaag
     2341 atgctcctag cggcaatgtg cttggccctt ctgggctgcc tacaggcgca ggaactcaag
     2401 ggtcatgttt ccataatcct gctgggagca accggggacc tagctaaaaa gtacctgtgg
     2461 cagggactgt ttcagctata cctggatgag gctgggaagg gccacagttt cagcttccac
     2521 ggtgctgccc tgacagcccc ccagcaaggc cagaagctca tggacaaggt cttggaatcc
     2581 ctctcctgtc ccaaggactt ggtacccagt cgctgtgatg aactcaaggg tcagtttcta
     2641 cagctgagcc aataccgcca gctgaagaca gtggaggact atcagaccct caacaaggat
     2701 attgagaccc aggtccagca ggatgggctc tgggaggcag gcaggatctt ctacttcagt
     2761 gtgccgccct ttgcctacgc agacattgcc cgtaacatca acagcagctg ccgaccacac
     2821 ccaggtgcct ggctacgggt tgtttttgaa aaaccctttg gccatgacca cctctcagcc
     2881 cagcagctgg cttcagaact tgggagcttt ttccaggagg aggagatgta ccgtgtggat
     2941 cattacctcg gcaagcaggc ggtggctcag atcctgccct tccgagatca gaaccgcaag
     3001 gccttggatg gcctctggaa caggcaccat gtggagcggg tggagatcat cttgaaggag
     3061 accatagatg cggaaggtcg ggccagtttc tatgaggagt atggcgtcat ccgtgacacg
     3121 ctgcagaacc acctgactga gatcctcacg ctggtggcca tggaactgcc tctcaacatc
     3181 agcagctcag ccgccgtgct ccagcataag ctctgggcct tccaggccct gcgggggctg
     3241 cagaagagca gtgccatcct gggccagtac caggcctaca gcggacaggt gcgccgagag
     3301 ctgcagaagc cagacggctt ccagagcctg acaccaacct tcgcaggtgt ccttgtccac
     3361 atcgacaacc tgcgctggga gggtgtccct ttcatcctga tgtctggcaa ggccttggat
     3421 gagagagtgg gctatgttcg gattgtgttt aagaatcggg cgtactgcac ccagagcgag
     3481 agacactggg tgccggagca gagccggtgc ctgccccagc agattatctt ctacattgga
     3541 cacggtgagc tgggccaccc tgccattctg gtcagccgga acctgttcaa accctcacta
     3601 cccactcaga agtggaagga ggtgcaggac cagcctgggc tccgcctgtt cggccgtcct
     3661 ctgtctgatt actacgccta caggcctgtg cgggagcaag acgcctactc caccctcctg
     3721 tcgcacatct tccactgccg aaaggagtcc ttcatcacca cagagaacct cttggcctcc
     3781 tgggtcttct ggaccccgtt actggacagc ctggcctttg aagttccacg cccataccca
     3841 gggggagccg agaatggcca gctgctggac tttgagttta gcggtggtca gctgaccttc
     3901 tcccagcagc agctggaggt gctgatacca gatctaggct cagtcccaaa gcccagtgac
     3961 ttccaggtcc tcggggcccg gtatagacag agcccactga ttaccgcctg gccagaggag
     4021 ctgatctcta agctggccag tgacattgaa gccgccgcgg tgcaggcagt acggcacttc
     4081 ggcaagttcc acctggcact gtcaggtggt tcgagcccca tagctctctt ccagcagctg
     4141 gccacaggac attacagctt cccctgggcc cacacacacc tctggctggt tgatgagcgt
     4201 tgtgtccctc tctcagatcc agactccaac ttccagggcc tgcaggctca cctgctacag
     4261 cacgtcaggg taccctacta caacatccac ccgatgccag tgcacctgca ccagcggctc
     4321 tgtgccgagg aggaccaggg agctcagacc tatgccagtg agatctcagc cctggtggcc
     4381 aacagcagct tcgacctggt gctcctgggc atgggcaccg acgggcacac agcttctctg
     4441 ttcccacagt cccccacagg cctggatggt gatcagctgg tggtgctgac ggagagcccg
     4501 ttcaggccac accaacgcat gagcctcagc ctgcccctca tcaaccgagc caagaaggta
     4561 gctgtcctgg tcatgggcag gacaaaacgt gagatcacaa cgttggtgag ccgcgtgggc
     4621 catgaaccca agaagtggcc catctcaggt gtggtgcctc tttctgggca gctggtttgg
     4681 tacatggatt acgaggcctt tctgggagga tccgactaca aagaccatga cggtgattat
     4741 aaagatcatg acatcgacta caaggatgac gatgacaagt agcggccgcg aaggatctgc
     4801 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     4861 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     4921 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     4981 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     5041 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     5101 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     5161 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     5221 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     5281 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta
     5341 gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc
     5401 accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag
     5461 cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac
     5521 ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac
     5581 gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag
     5641 tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg
     5701 atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac
     5761 aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg
     5821 gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg
     5881 gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc
     5941 atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag
     6001 taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat
     6061 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt
     6121 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg
     6181 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     6241 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     6301 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     6361 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     6421 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     6481 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     6541 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     6601 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     6661 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     6721 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa
     6781 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6841 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     6901 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     6961 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7021 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7081 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7141 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     7201 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7261 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     7321 cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc
     7381 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     7441 ccaacgaaga taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     7501 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7561 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7621 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     7681 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     7741 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7801 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     7861 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     7921 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     7981 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac
     8041 caaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8101 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8161 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8221 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8281 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8341 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8401 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8461 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8521 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8581 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8641 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8701 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8761 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8821 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8881 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8941 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9001 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9061 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9121 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9181 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9241 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9301 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9361 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9421 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9481 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9541 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9601 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9661 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9721 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9781 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9841 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9901 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9961 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10021 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10081 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10141 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10201 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    10261 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    10321 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    10381 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
    10441 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    10501 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    10561 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    10621 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
    10681 g
//
