LOCUS       V028793                10903 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028793
VERSION     V028793
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4929
                     /label="Grm6(NM_173372)"
                     /note="Grm6(NM_173372)"
                     /gene="Grm6"
     CDS             4936..5001
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5045..5256
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5269..5537
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5566..5575
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5596..6258
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6328..6381
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6382..6981
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6982..7570
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7644..7877
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7949..8070
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8110..8245
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8278..8294)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8302..8318
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8326..8356)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8371..8392
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8680..9268)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9439..10299)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10300..10404)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10878..10894
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ggcggctccg agtgctgctg
     2341 ctgtggctag cctggtggct gtcgcaggcg ggcatagctc acggtgcggg ctcggtgcgc
     2401 ctggcaggcg gcctgacgct gggcggcctc ttcccggtgc acgcccgggg cgccgcaggg
     2461 cgtgcgtgcg ggacgctgaa gaaggagcag ggagtgcacc ggctggaggc gatgctctac
     2521 gcgctggacc gcatcaacgc cgacccggag ctcctgcccg gtgtgcgact aggcgcgcgg
     2581 ctgctcgaca cctgctcgcg ggacacctac gctctggagc aggcgctgag cttcgtgcag
     2641 gcgctcatcc gaggccgcgg agacggcgaa gaggccagcg tgcgctgccc tgggggcgtc
     2701 cccccgctgc gcgccgcgcc tcctgaacgc gtcgtggcgg tggtgggcgc ctcagctagc
     2761 tccgtgtcca tcatggtcgc caatgtcttg cgcctgtttg cgatacccca gatcagctat
     2821 gcctccacag ccccagagct cagtgactcc acacgctatg acttcttctc ccgagtggtg
     2881 cctcctgact cctaccaggc gcaggccatg gtggacattg tgcgggcact gggatggaat
     2941 tacgtgtcta cactggcctc tgagggcaac tatggtgaaa gcggggttga ggcctttgtg
     3001 cagatctccc gtgaggctgg aggtgtctgt attgcccaat caataaagat tcccagggaa
     3061 ccaaagccag gagaattcca caaagtgatc cggaggctca tggagacacc caacgcccgg
     3121 ggcatcatca tctttgccaa tgaagatgac atcaggaggg tcttggaggc aacacgccag
     3181 gcaaacctga ctggccactt cctgtgggtt ggctcagaca gctggggatc caagatctct
     3241 cccatcttga acctagagga agaggctgtg ggggccatca ccatcttgcc caaaagggct
     3301 tcaatcgacg gatttgacca atactttatg actcgttccc tggagaacaa ccgcagaaac
     3361 atctggtttg ctgagttctg ggaagagaat tttaactgca aactaaccag ctcaggtggc
     3421 cagtcagatg attccacccg gaaatgcaca ggcgaggaac gcatcggcca ggactccacc
     3481 tatgaacagg aggggaaggt gcagttcgtg atagatgctg tgtatgccat cgctcatgct
     3541 ctgcacagca tgcaccaagc actctgccca ggccacacag gtctgtgccc agccatggag
     3601 cctactgatg gccggaccct gctgcactac atccgagcag tccgcttcaa tggtagcgca
     3661 ggaaccccag tgatgttcaa tgagaatggg gatgcccccg ggcgctatga catcttccag
     3721 taccaagcaa ccaatggaag tgccagcagt ggcgggtacc aggctgtggg ccaatgggca
     3781 gaggccctta gactggatat ggaagctcta cagtggtcag gcgaccccca tgaggtgccc
     3841 ccttctcaat gcagcctccc ctgtgggcct ggtgaacgga aaaagatggt gaagggtgtg
     3901 ccctgctgtt ggcactgtga ggcctgcgac gggtaccgct tccaggtgga cgagttcacg
     3961 tgtgaggcct gtccagggca catgagaccg acacccaacc acactggctg ccgtcccact
     4021 cccgtggtgc gcctgacctg gtcctctccg tgggccgctc tgccccttct cctggctgtc
     4081 ctgggcatca tggccaccac aactatcatt gctaccttca tgcgacacaa cgacactccc
     4141 atagtccgcg cctctggccg tgagcttagc tatgtgctgc tcaccggcat cttcctgatc
     4201 tacgccatca ccttcctcat ggtagccgag ccttgtgcag ccgtctgtgc ttcccgaaga
     4261 ctcttgctcg gcttgggtac cacgctcagc tactctgccc tgctcaccaa gaccaaccgc
     4321 atctaccgca ttttcgagca agggaagcgc tctgtcacgc cgccaccctt catcagcccc
     4381 acctcgcagc tagtcatcac cttcggcctt acctccctgc aggtagtggg agtgatagcg
     4441 tggttggggg cccaacctcc acacagcgtg attgactatg aggaacagag gacggtggac
     4501 cctgagcaag ccaggggtgt gctcaagtgt gacatgtctg atctgtccct cattggctgc
     4561 ctggggtaca gtctcctgct catggtcacg tgtacggtgt atgccatcaa ggcccgaggt
     4621 gtgccagaga ccttcaatga agccaagccc atcggcttca ccatgtacac cacctgtatt
     4681 atctggctgg ctttcgtccc catcttcttt ggcaccgccc agtcagctga gaagatctac
     4741 atccaaacga ccacactgac tgtgtccctg agcctgagcg cgtcggtgtc cctcggcatg
     4801 ctctatgtgc ccaaaaccta cgtcatcctg ttccatccag agcagaacgt gcagaagcgg
     4861 aagcgcagcc tcaagaagac ctccacgatg gcggccccgc ccaagagcga gaactcagag
     4921 gacgccaagg gatccgacta caaagaccat gacggtgatt ataaagatca tgacatcgac
     4981 tacaaggatg acgatgacaa gtagcggccg cgaaggatct gcgatcgctc cggtgcccgt
     5041 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     5101 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     5161 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     5221 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     5281 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     5341 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     5401 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     5461 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     5521 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc
     5581 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc
     5641 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac
     5701 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg
     5761 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac
     5821 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg
     5881 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg
     5941 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac
     6001 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc
     6061 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc
     6121 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc
     6181 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag
     6241 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc
     6301 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac
     6361 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc
     6421 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     6481 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     6541 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     6601 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     6661 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     6721 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     6781 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     6841 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     6901 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     6961 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact
     7021 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7081 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7141 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7201 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7261 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7321 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7381 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7441 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7501 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7561 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7621 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct
     7681 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7741 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7801 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7861 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     7921 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     7981 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     8041 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     8101 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8161 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8221 tagtgaggag gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc
     8281 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8341 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8401 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8461 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8521 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8581 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8641 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8701 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8761 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8821 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8881 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8941 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9001 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9061 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9121 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9181 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9241 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9301 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9361 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9421 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9481 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9541 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9601 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9661 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9721 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9781 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9841 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9901 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9961 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10021 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10081 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10141 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10201 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10261 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10321 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10381 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    10441 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
    10501 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
    10561 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    10621 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    10681 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
    10741 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    10801 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    10861 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
