LOCUS V039298 9494 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039298 VERSION V039298 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..3495 /label="Golm1(NM_027307)" /note="Golm1(NM_027307)" /gene="Golm1" CDS 3502..3528 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3532..3558 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3562..3588 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 3653..3864 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3877..4145 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 4174..4183 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 4204..4866 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 4936..4989 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 4990..5589 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5590..6177 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6251..6484 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6556..6677 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6696..6831 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6869..6885) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6893..6909 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6917..6947) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6962..6983 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7271..7859) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8030..8890) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8891..8995) /gene="bla" /label="AmpR promoter" primer_bind 9469..9485 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga tgggattggg gaatgggcgt 2341 cgcagcatga agtctccgcc ccttatcctg gctgccctgg tggcctgtgt tattgtgcta 2401 ggcttcaact actggattgc tagctcgaga agcgtggaac tccagacgcg cattgtagag 2461 ctggaaggcc gggtccgcag ggcggctgca gagagaggtg ctgtagagct caagaagaat 2521 gagttccagg gcgagctgca gaagcagaga gagcaacttg acaggatcca gtctagccac 2581 agcttccagc tggagaatgt caacaagcta caccaggatg agaaggcggt tttagtgaat 2641 aacatcacca caggcgagaa gctcattcga gacctgcaag accagttgaa ggcactgcag 2701 aggagttaca gcagcctgca gcaggatatc ttccaattcc agaagaacca gaccagcctg 2761 gagaagaagt tctcctatga cctgaaccag tgtatcagcc agatgaccga ggtgaaggaa 2821 cagtgtgacg agcggataga ggaggtcatc agaaagcgaa acgaagcacc gggttccaga 2881 gacctggctg aaaccaacaa ccaacatcag caggccctca agcctcagcc caagcttcag 2941 gaagaagtcc catcagagga acagatgcca caagagaaag gggatgtgcc cagaaacaag 3001 tcccagattc cagcccccaa ttcagagagc ttgggtttga agccacaggt gcagaatgag 3061 gaaaccaatg agattcaggc tgtcggtgag gagcaccaac aggcatccat ccaagggcag 3121 gcggtggcgg atggcactcg ggtaggagct gagaaactcg atcagcacac acagctacca 3181 gctggcctgt tggccaggcc tgaggaggac tctcagtacc ctgagcggga gcagcttgtc 3241 atccgggata ggcaggagca gcagcgtgca tcagaggaag ggggaggcca gaagaaccca 3301 ggagatgaat acgacatgga tgaaaacgaa gcagaatctg agagggagaa gcaggcagcc 3361 cttgcaggga atgacagaaa tataaatgtt cttaatgctg acgctcagaa gagaggcata 3421 ataaatgtgc ccgtcgggag tgaaaggcag agccacattc taaatcaagt aggcattcat 3481 attcctcaac aagccctcga gtacccatac gacgtacctg attatgcaac ttatccttat 3541 gatgtacctg attatgctag ctatccttat gatgtcccag actacgcata gggatccctg 3601 cgatatccag cgcggccgcg aaggatctgc gatcgctccg gtgcccgtca gtgggcagag 3661 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc 3721 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 3781 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 3841 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc 3901 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc 3961 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga 4021 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt 4081 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac 4141 agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc 4201 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag 4261 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc 4321 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc 4381 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac 4441 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc 4501 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc 4561 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag 4621 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg 4681 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc 4741 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg 4801 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc 4861 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc 4921 tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat 4981 cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc 5041 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc 5101 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc 5161 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc 5221 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag 5281 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg 5341 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag 5401 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc 5461 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc 5521 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc 5581 ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 5641 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 5701 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 5761 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 5821 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 5881 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 5941 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca 6001 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 6061 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 6121 ccgcgtctcc gccttcgccc tcagacgagt cggatctccc tttggccgcc tccccgcctg 6181 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 6241 aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt 6301 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 6361 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 6421 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 6481 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata 6541 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc 6601 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 6661 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt 6721 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 6781 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt 6841 gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6901 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 6961 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 7021 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 7081 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 7141 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 7201 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 7261 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 7321 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 7381 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 7441 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 7501 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 7561 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7621 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7681 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 7741 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7801 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7861 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 7921 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7981 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 8041 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 8101 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 8161 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 8221 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 8281 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 8341 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 8401 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 8461 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 8521 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 8581 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8641 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8701 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8761 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8821 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8881 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8941 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 9001 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata 9061 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc 9121 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 9181 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg 9241 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat 9301 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg 9361 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg 9421 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 9481 ccagtgccaa gctg //