LOCUS V038113 9305 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038113 VERSION V038113 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..3399 /label="GPR183(NM_004951)" /note="GPR183(NM_004951)" /gene="GPR183" promoter 3464..3675 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3688..3956 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 3985..3994 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 4015..4677 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 4747..4800 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 4801..5400 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5401..5988 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6062..6295 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6367..6488 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6507..6642 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6680..6696) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6704..6720 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6728..6758) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6773..6794 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7082..7670) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7841..8701) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8702..8806) /gene="bla" /label="AmpR promoter" primer_bind 9280..9296 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg atatacaaat ggcaaacaat 2341 tttactccgc cctctgcaac tcctcaggga aatgactgtg acctctatgc acatcacagc 2401 acggccagga tagtaatgcc tctgcattac agcctcgtct tcatcattgg gctcgtggga 2461 aacttactag ccttggtcgt cattgttcaa aacaggaaaa aaatcaactc taccaccctc 2521 tattcaacaa atttggtgat ttctgatata ctttttacca ccgctttgcc tacacgaata 2581 gcctactatg caatgggctt tgactggaga atcggagatg ccttgtgtag gataactgcg 2641 ctagtgtttt acatcaacac atatgcaggt gtgaacttta tgacctgcct gagtattgac 2701 cgcttcattg ctgtggtgca ccctctacgc tacaacaaga taaaaaggat tgaacatgca 2761 aaaggcgtgt gcatatttgt ctggattcta gtatttgctc agacactccc actcctcatc 2821 aaccctatgt caaagcagga ggctgaaagg attacatgca tggagtatcc aaactttgaa 2881 gaaactaaat ctcttccctg gattctgctt ggggcatgtt tcataggata tgtacttcca 2941 cttataatca ttctcatctg ctattctcag atctgctgca aactcttcag aactgccaaa 3001 caaaacccac tcactgagaa atctggtgta aacaaaaagg ctctcaacac aattattctt 3061 attattgttg tgtttgttct ctgtttcaca ccttaccatg ttgcaattat tcaacatatg 3121 attaagaagc ttcgtttctc taatttcctg gaatgtagcc aaagacattc gttccagatt 3181 tctctgcact ttacagtatg cctgatgaac ttcaattgct gcatggaccc ttttatctac 3241 ttctttgcat gtaaagggta taagagaaag gttatgagga tgctgaaacg gcaagtcagt 3301 gtatcgattt ctagtgctgt gaagtcagcc cctgaagaaa attcacgtga aatgacagaa 3361 acgcagatga tgatacattc caagtcttca aatggaaagt gaggatccct gcgatatcca 3421 gcgcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 3481 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 3541 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 3601 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 3661 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 3721 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 3781 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 3841 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 3901 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 3961 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc 4021 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc 4081 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc 4141 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc 4201 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac 4261 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac 4321 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag 4381 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac 4441 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc 4501 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc 4561 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac 4621 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga 4681 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 4741 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 4801 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 4861 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 4921 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 4981 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 5041 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 5101 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 5161 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 5221 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 5281 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 5341 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 5401 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 5461 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 5521 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 5581 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 5641 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 5701 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 5761 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc 5821 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 5881 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc 5941 cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct ggtaccttta 6001 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 6061 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct 6121 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 6181 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 6241 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 6301 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 6361 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 6421 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 6481 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 6541 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 6601 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 6661 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 6721 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 6781 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 6841 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 6901 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 6961 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 7021 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 7081 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 7141 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 7201 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 7261 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 7321 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 7381 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 7441 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 7501 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 7561 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 7621 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 7681 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 7741 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 7801 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 7861 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 7921 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 7981 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 8041 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 8101 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 8161 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 8221 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 8281 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 8341 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 8401 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 8461 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 8521 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 8581 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 8641 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 8701 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 8761 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 8821 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 8881 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 8941 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 9001 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 9061 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 9121 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 9181 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 9241 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 9301 agctg //