LOCUS       V032129                10254 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032129
VERSION     V032129
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4336
                     /label="GAS6(NM_000820)"
                     /note="GAS6(NM_000820)"
                     /gene="GAS6"
     CDS             4343..4408
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4452..4663
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4676..4944
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             4979..5686
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5696..5749
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5750..6349
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6350..6937
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7011..7244
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7316..7437
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7456..7591
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7629..7645)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7653..7669
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7677..7707)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7722..7743
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8031..8619)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8790..9650)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9651..9755)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10229..10245
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggcccc ttcgctctcg cccgggcccg ccgccctgcg
     2341 ccgcgcgccg cagctgctgc tgctgctgct ggccgcggag tgcgcgcttg ccgcgctgtt
     2401 gccggcgcgc gaggccacgc agttcctgcg gcccaggcag cgccgcgcct ttcaggtctt
     2461 cgaggaggcc aagcagggcc acctggagag ggagtgcgtg gaggagctgt gcagccgcga
     2521 ggaggcgcgg gaggtgttcg agaacgaccc cgagacggat tatttttacc caagatactt
     2581 agactgcatc aacaagtatg ggtctccgta caccaaaaac tcaggcttcg ccacctgcgt
     2641 gcaaaacctg cctgaccagt gcacgcccaa cccctgcgat aggaagggga cccaagcctg
     2701 ccaggacctc atgggcaact tcttctgcct gtgtaaagct ggctgggggg gccggctctg
     2761 cgacaaagat gtcaacgaat gcagccagga gaacgggggc tgcctccaga tctgccacaa
     2821 caagccgggt agcttccact gttcctgcca cagcggcttc gagctctcct ctgatggcag
     2881 gacctgccaa gacatagacg agtgcgcaga ctcggaggcc tgcggggagg cgcgctgcaa
     2941 gaacctgccc ggctcctact cctgcctctg tgacgagggc tttgcgtaca gctcccagga
     3001 gaaggcttgc cgagatgtgg acgagtgtct gcagggccgc tgtgagcagg tctgcgtgaa
     3061 ctccccaggg agctacacct gccactgtga cgggcgtggg ggcctcaagc tgtcccagga
     3121 catggacacc tgtgaggaca tcttgccgtg cgtgcccttc agcgtggcca agagtgtgaa
     3181 gtccttgtac ctgggccgga tgttcagtgg gacccccgtg atccgactgc gcttcaagag
     3241 gctgcagccc accaggctgg tagctgagtt tgacttccgg acctttgacc ccgagggcat
     3301 cctcctcttt gccggaggcc accaggacag cacctggatc gtgctggccc tgagagccgg
     3361 ccggctggag ctgcagctgc gctacaacgg tgtcggccgt gtcaccagca gcggcccggt
     3421 catcaaccat ggcatgtggc agacaatctc tgttgaggag ctggcgcgga atctggtcat
     3481 caaggtcaac agggatgctg tcatgaaaat cgcggtggcc ggggacttgt tccaaccgga
     3541 gcgaggactg tatcatctga acctgaccgt gggaggtatt cccttccatg agaaggacct
     3601 cgtgcagcct ataaaccctc gtctggatgg ctgcatgagg agctggaact ggctgaacgg
     3661 agaagacacc accatccagg aaacggtgaa agtgaacacg aggatgcagt gcttctcggt
     3721 gacggagaga ggctctttct accccgggag cggcttcgcc ttctacagcc tggactacat
     3781 gcggacccct ctggacgtcg ggactgaatc aacctgggaa gtagaagtcg tggctcacat
     3841 ccgcccagcc gcagacacag gcgtgctgtt tgcgctctgg gcccccgacc tccgtgccgt
     3901 gcctctctct gtggcactgg tagactatca ctccacgaag aaactcaaga agcagctggt
     3961 ggtcctggcc gtggagcata cggccttggc cctaatggag atcaaggtct gcgacggcca
     4021 agagcacgtg gtcaccgtct cgctgaggga cggtgaggcc accctggagg tggacggcac
     4081 caggggccag agcgaggtga gcgccgcgca gctgcaggag aggctggccg tgctcgagag
     4141 gcacctgcgg agccccgtgc tcacctttgc tggcggcctg ccagatgtgc cggtgacttc
     4201 agcgccagtc accgcgttct accgcggctg catgacactg gaggtcaacc ggaggctgct
     4261 ggacctggac gaggcggcgt acaagcacag cgacatcacg gcccactcct gcccccccgt
     4321 ggagcccgcc gcagccggat ccgactacaa agaccatgac ggtgattata aagatcatga
     4381 catcgactac aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg
     4441 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     4501 cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     4561 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     4621 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc
     4681 tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag
     4741 tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag
     4801 tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact
     4861 cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt
     4921 ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat
     4981 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     5041 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     5101 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     5161 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     5221 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     5281 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     5341 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     5401 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     5461 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     5521 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     5581 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     5641 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagggat ctcgcgaggg
     5701 cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta
     5761 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     5821 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     5881 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     5941 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     6001 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     6061 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     6121 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     6181 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     6241 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     6301 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct
     6361 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6421 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6481 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6541 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6601 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6661 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6721 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     6781 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6841 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc gccttcgccc
     6901 tcagacgagt cggatctccc tttggccgcc tccccgcctg gtacctttaa gaccaatgac
     6961 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7021 aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga
     7081 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7141 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7201 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     7261 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     7321 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7381 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7441 tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7501 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7561 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg
     7621 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7681 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7741 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7801 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7861 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7921 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7981 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8041 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8101 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8161 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8221 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8281 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8341 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8401 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8461 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8521 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8581 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8641 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8701 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8761 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8821 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8881 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8941 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     9001 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     9061 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     9121 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9181 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9241 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9301 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9361 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9421 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9481 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9541 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9601 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9661 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9721 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9781 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     9841 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     9901 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     9961 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
    10021 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
    10081 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
    10141 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
    10201 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//
