LOCUS       V039660                11017 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039660
VERSION     V039660
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2283..4067
                     /label="TNFRSF8(NM_001243)"
                     /note="TNFRSF8(NM_001243)"
                     /gene="TNFRSF8"
     CDS             4074..4139
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4189..4400
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4413..4681
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4710..4719
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4740..6389
                     /EC_number=""
                     /codon_start=1
                     /gene="luc+"
                     /note="enhanced&nbsp;luc+ version of the luciferase gene"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label="luciferase"
     CDS             6459..6512
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6513..7112
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7113..7700
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7774..8007
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8079..8200
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8219..8354
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8392..8408)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8416..8432
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8440..8470)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8485..8506
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8794..9382)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9553..10413)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10414..10518)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10992..11008
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag gaattcgcca
     2281 ccatgcgcgt cctcctcgcc gcgctgggac tgctgttcct gggggcgcta cgagccttcc
     2341 cacaggatcg acccttcgag gacacctgtc atggaaaccc cagccactac tatgacaagg
     2401 ctgtcaggag gtgctgttac cgctgcccca tggggctgtt cccgacacag cagtgcccac
     2461 agaggcctac tgactgcagg aagcagtgtg agcctgacta ctacctggat gaggccgacc
     2521 gctgtacagc ctgcgtgact tgttctcgag acgacctcgt ggagaagacg ccgtgtgcat
     2581 ggaactcctc ccgtgtctgc gaatgtcgac ccggcatgtt ctgttccacg tctgccgtca
     2641 actcctgtgc ccgctgcttc ttccattctg tctgtccggc agggatgatt gtcaagttcc
     2701 caggcacggc gcagaagaac acggtctgtg agccggcttc cccaggggtc agccctgcct
     2761 gtgccagccc agagaactgc aaggaaccct ccagtggcac catcccccag gccaagccca
     2821 ccccggtgtc cccagcaacc tccagtgcca gcaccatgcc tgtaagaggg ggcacccgcc
     2881 tcgcccagga agctgcttct aaactgacga gggctcccga ctctccctcc tctgtgggaa
     2941 ggcctagttc agatccaggt ctgtccccaa cacagccatg cccagagggg tctggtgatt
     3001 gcagaaagca gtgtgagccc gactactacc tggacgaggc cggccgctgc acggcctgcg
     3061 tgagctgttc tcgagatgac cttgtggaga agacgccatg tgcatggaac tcctcccgca
     3121 cctgcgaatg tcgacctggc atgatctgtg ccacatcagc caccaactcc tgtgcccgct
     3181 gtgtccccta cccaatctgt gcagcagaga cggtcaccaa gccccaggat atggctgaga
     3241 aggacaccac ctttgaggcg ccacccctgg ggacccagcc ggactgcaac cccaccccag
     3301 agaatggcga ggcgcctgcc agcaccagcc ccactcagag cttgctggtg gactcccagg
     3361 ccagtaagac gctgcccatc ccaaccagcg ctcccgtcgc tctctcctcc acggggaagc
     3421 ccgttctgga tgcagggcca gtgctcttct gggtgatcct ggtgttggtt gtggtggtcg
     3481 gctccagcgc cttcctcctg tgccaccgga gggcctgcag gaagcgaatt cggcagaagc
     3541 tccacctgtg ctacccggtc cagacctccc agcccaagct agagcttgtg gattccagac
     3601 ccaggaggag ctcaacgcag ctgaggagtg gtgcgtcggt gacagaaccc gtcgcggaag
     3661 agcgagggtt aatgagccag ccactgatgg agacctgcca cagcgtgggg gcagcctacc
     3721 tggagagcct gccgctgcag gatgccagcc cggccggggg cccctcgtcc cccagggacc
     3781 ttcctgagcc ccgggtgtcc acggagcaca ccaataacaa gattgagaaa atctacatca
     3841 tgaaggctga caccgtgatc gtggggaccg tgaaggctga gctgccggag ggccggggcc
     3901 tggcggggcc agcagagccc gagttggagg aggagctgga ggcggaccat accccccact
     3961 accccgagca ggagacagaa ccgcctctgg gcagctgcag cgatgtcatg ctctcagtgg
     4021 aagaggaagg gaaagaagac cccttgccca cagctgcctc tggaaagctc gaggactaca
     4081 aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt
     4141 aaggatccgc ggccgcaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca
     4201 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga
     4261 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     4321 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     4381 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc
     4441 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct
     4501 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg
     4561 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc
     4621 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat
     4681 ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag agcggcctga
     4741 tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa
     4801 ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg
     4861 cttttacaga tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg
     4921 ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat
     4981 gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg
     5041 cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt
     5101 cgcagcctac cgtggtgttc gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa
     5161 aaaagctccc aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat
     5221 ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt
     5281 ttgtgccaga gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat
     5341 ctactggtct gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc
     5401 atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg
     5461 ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc
     5521 gagtcgtctt aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca
     5581 agattcaaag tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga
     5641 ttgacaaata cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta
     5701 aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg
     5761 ggctcactga gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg
     5821 cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa
     5881 cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt
     5941 atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg
     6001 gagacatagc ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc
     6061 tgattaagta caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac
     6121 accccaacat cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc
     6181 ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt
     6241 acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg
     6301 aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa
     6361 aggccaagaa gggcggaaag atcgccgtga gatcccgcgc tcagtcgtcc aattctgccg
     6421 tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga agtcttctaa
     6481 catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc acggtgcgcc
     6541 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     6601 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     6661 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     6721 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     6781 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     6841 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     6901 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     6961 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     7021 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     7081 cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac aaaatttgtg
     7141 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     7201 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     7261 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     7321 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     7381 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7441 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7501 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7561 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7621 tgctgccggc tctgcggcct cttccgcgtc tccgccttcg ccctcagacg agtcggatct
     7681 ccctttggcc gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag
     7741 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     7801 aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     7861 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7921 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7981 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     8041 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     8101 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     8161 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     8221 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8281 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     8341 cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat ggtcatagct
     8401 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     8461 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     8521 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     8581 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     8641 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8701 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8761 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8821 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8881 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8941 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     9001 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     9061 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     9121 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     9181 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     9241 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     9301 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9361 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9421 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9481 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     9541 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     9601 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9661 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9721 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9781 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9841 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9901 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9961 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    10021 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    10081 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    10141 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    10201 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    10261 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    10321 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    10381 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    10441 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    10501 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
    10561 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
    10621 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
    10681 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
    10741 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    10801 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
    10861 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
    10921 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
    10981 gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
