LOCUS       V034612                16844 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034612
VERSION     V034612
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..9966
                     /label="NOTCH1(NM_017617)"
                     /note="NOTCH1(NM_017617)"
                     /gene="NOTCH1"
     promoter        10016..10227
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             10240..10508
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      10537..10546
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             10567..12216
                     /EC_number=""
                     /codon_start=1
                     /gene="luc+"
                     /note="enhanced&nbsp;luc+ version of the luciferase gene"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label="luciferase"
     CDS             12286..12339
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             12340..12939
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    12940..13527
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             13601..13834
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    13906..14027
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      14046..14181
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(14219..14235)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    14243..14259
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(14267..14297)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    14312..14333
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(14621..15209)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(15380..16240)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(16241..16345)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     16819..16835
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgccgccg ctcctggcgc ccctgctctg cctggcgctg
     2341 ctgcccgcgc tcgccgcacg aggcccgcga tgctcccagc ccggtgagac ctgcctgaat
     2401 ggcgggaagt gtgaagcggc caatggcacg gaggcctgcg tctgtggcgg ggccttcgtg
     2461 ggcccgcgat gccaggaccc caacccgtgc ctcagcaccc cctgcaagaa cgccgggaca
     2521 tgccacgtgg tggaccgcag aggcgtggca gactatgcct gcagctgtgc cctgggcttc
     2581 tctgggcccc tctgcctgac acccctggac aatgcctgcc tcaccaaccc ctgccgcaac
     2641 gggggcacct gcgacctgct cacgctgacg gagtacaagt gccgctgccc gcccggctgg
     2701 tcagggaaat cgtgccagca ggctgacccg tgcgcctcca acccctgcgc caacggtggc
     2761 cagtgcctgc ccttcgaggc ctcctacatc tgccactgcc cacccagctt ccatggcccc
     2821 acctgccggc aggatgtcaa cgagtgtggc cagaagcccg ggctttgccg ccacggaggc
     2881 acctgccaca acgaggtcgg ctcctaccgc tgcgtctgcc gcgccaccca cactggcccc
     2941 aactgcgagc ggccctacgt gccctgcagc ccctcgccct gccagaacgg gggcacctgc
     3001 cgccccacgg gcgacgtcac ccacgagtgt gcctgcctgc caggcttcac cggccagaac
     3061 tgtgaggaaa atatcgacga ttgtccagga aacaactgca agaacggggg tgcctgtgtg
     3121 gacggcgtga acacctacaa ctgccgctgc ccgccagagt ggacaggtca gtactgtacc
     3181 gaggatgtgg acgagtgcca gctgatgcca aatgcctgcc agaacggcgg gacctgccac
     3241 aacacccacg gtggctacaa ctgcgtgtgt gtcaacggct ggactggtga ggactgcagc
     3301 gagaacattg atgactgtgc cagcgccgcc tgcttccacg gcgccacctg ccatgaccgt
     3361 gtggcctcct tctactgcga gtgtccccat ggccgcacag gtctgctgtg ccacctcaac
     3421 gacgcatgca tcagcaaccc ctgtaacgag ggctccaact gcgacaccaa ccctgtcaat
     3481 ggcaaggcca tctgcacctg cccctcgggg tacacgggcc cggcctgcag ccaggacgtg
     3541 gatgagtgct cgctgggtgc caacccctgc gagcatgcgg gcaagtgcat caacacgctg
     3601 ggctccttcg agtgccagtg tctgcagggc tacacgggcc cccgatgcga gatcgacgtc
     3661 aacgagtgcg tctcgaaccc gtgccagaac gacgccacct gcctggacca gattggggag
     3721 ttccagtgca tctgcatgcc cggctacgag ggtgtgcact gcgaggtcaa cacagacgag
     3781 tgtgccagca gcccctgcct gcacaatggc cgctgcctgg acaagatcaa tgagttccag
     3841 tgcgagtgcc ccacgggctt cactgggcat ctgtgccagt acgatgtgga cgagtgtgcc
     3901 agcaccccct gcaagaatgg tgccaagtgc ctggacggac ccaacactta cacctgtgtg
     3961 tgcacggaag ggtacacggg gacgcactgc gaggtggaca tcgatgagtg cgaccccgac
     4021 ccctgccact acggctcctg caaggacggc gtcgccacct tcacctgcct ctgccgccca
     4081 ggctacacgg gccaccactg cgagaccaac atcaacgagt gctccagcca gccctgccgc
     4141 cacgggggca cctgccagga ccgcgacaac gcctacctct gcttctgcct gaaggggacc
     4201 acaggaccca actgcgagat caacctggat gactgtgcca gcagcccctg cgactcgggc
     4261 acctgtctgg acaagatcga tggctacgag tgtgcctgtg agccgggcta cacagggagc
     4321 atgtgtaaca tcaacatcga tgagtgtgcg ggcaacccct gccacaacgg gggcacctgc
     4381 gaggacggca tcaatggctt cacctgccgc tgccccgagg gctaccacga ccccacctgc
     4441 ctgtctgagg tcaatgagtg caacagcaac ccctgcgtcc acggggcctg ccgggacagc
     4501 ctcaacgggt acaagtgcga ctgtgaccct gggtggagtg ggaccaactg tgacatcaac
     4561 aacaatgagt gtgaatccaa cccttgtgtc aacggcggca cctgcaaaga catgaccagt
     4621 ggctacgtgt gcacctgccg ggagggcttc agcggtccca actgccagac caacatcaac
     4681 gagtgtgcgt ccaacccatg tctgaaccag ggcacgtgta ttgacgacgt tgccgggtac
     4741 aagtgcaact gcctgctgcc ctacacaggt gccacgtgtg aggtggtgct ggccccgtgt
     4801 gcccccagcc cctgcagaaa cggcggggag tgcaggcaat ccgaggacta tgagagcttc
     4861 tcctgtgtct gccccacggg ctggcaaggg cagacctgtg aggtcgacat caacgagtgc
     4921 gttctgagcc cgtgccggca cggcgcatcc tgccagaaca cccacggcgg ctaccgctgc
     4981 cactgccagg ccggctacag tgggcgcaac tgcgagaccg acatcgacga ctgccggccc
     5041 aacccgtgtc acaacggggg ctcctgcaca gacggcatca acacggcctt ctgcgactgc
     5101 ctgcccggct tccggggcac tttctgtgag gaggacatca acgagtgtgc cagtgacccc
     5161 tgccgcaacg gggccaactg cacggactgc gtggacagct acacgtgcac ctgccccgca
     5221 ggcttcagcg ggatccactg tgagaacaac acgcctgact gcacagagag ctcctgcttc
     5281 aacggtggca cctgcgtgga cggcatcaac tcgttcacct gcctgtgtcc acccggcttc
     5341 acgggcagct actgccagca cgatgtcaat gagtgcgact cacagccctg cctgcatggc
     5401 ggcacctgtc aggacggctg cggctcctac aggtgcacct gcccccaggg ctacactggc
     5461 cccaactgcc agaaccttgt gcactggtgt gactcctcgc cctgcaagaa cggcggcaaa
     5521 tgctggcaga cccacaccca gtaccgctgc gagtgcccca gcggctggac cggcctttac
     5581 tgcgacgtgc ccagcgtgtc ctgtgaggtg gctgcgcagc gacaaggtgt tgacgttgcc
     5641 cgcctgtgcc agcatggagg gctctgtgtg gacgcgggca acacgcacca ctgccgctgc
     5701 caggcgggct acacaggcag ctactgtgag gacctggtgg acgagtgctc acccagcccc
     5761 tgccagaacg gggccacctg cacggactac ctgggcggct actcctgcaa gtgcgtggcc
     5821 ggctaccacg gggtgaactg ctctgaggag atcgacgagt gcctctccca cccctgccag
     5881 aacgggggca cctgcctcga cctccccaac acctacaagt gctcctgccc acggggcact
     5941 cagggtgtgc actgtgagat caacgtggac gactgcaatc cccccgttga ccccgtgtcc
     6001 cggagcccca agtgctttaa caacggcacc tgcgtggacc aggtgggcgg ctacagctgc
     6061 acctgcccgc cgggcttcgt gggtgagcgc tgtgaggggg atgtcaacga gtgcctgtcc
     6121 aatccctgcg acgcccgtgg cacccagaac tgcgtgcagc gcgtcaatga cttccactgc
     6181 gagtgccgtg ctggtcacac cgggcgccgc tgcgagtccg tcatcaatgg ctgcaaaggc
     6241 aagccctgca agaatggggg cacctgcgcc gtggcctcca acaccgcccg cgggttcatc
     6301 tgcaagtgcc ctgcgggctt cgagggcgcc acgtgtgaga atgacgctcg tacctgcggc
     6361 agcctgcgct gcctcaacgg cggcacatgc atctccggcc cgcgcagccc cacctgcctg
     6421 tgcctgggcc ccttcacggg ccccgaatgc cagttcccgg ccagcagccc ctgcctgggc
     6481 ggcaacccct gctacaacca ggggacctgt gagcccacat ccgagagccc cttctaccgt
     6541 tgcctgtgcc ccgccaaatt caacgggctc ttgtgccaca tcctggacta cagcttcggg
     6601 ggtggggccg ggcgcgacat ccccccgccg ctgatcgagg aggcgtgcga gctgcccgag
     6661 tgccaggagg acgcgggcaa caaggtctgc agcctgcagt gcaacaacca cgcgtgcggc
     6721 tgggacggcg gtgactgctc cctcaacttc aatgacccct ggaagaactg cacgcagtct
     6781 ctgcagtgct ggaagtactt cagtgacggc cactgtgaca gccagtgcaa ctcagccggc
     6841 tgcctcttcg acggctttga ctgccagcgt gcggaaggcc agtgcaaccc cctgtacgac
     6901 cagtactgca aggaccactt cagcgacggg cactgcgacc agggctgcaa cagcgcggag
     6961 tgcgagtggg acgggctgga ctgtgcggag catgtacccg agaggctggc ggccggcacg
     7021 ctggtggtgg tggtgctgat gccgccggag cagctgcgca acagctcctt ccacttcctg
     7081 cgggagctca gccgcgtgct gcacaccaac gtggtcttca agcgtgacgc acacggccag
     7141 cagatgatct tcccctacta cggccgcgag gaggagctgc gcaagcaccc catcaagcgt
     7201 gccgccgagg gctgggccgc acctgacgcc ctgctgggcc aggtgaaggc ctcgctgctc
     7261 cctggtggca gcgagggtgg gcggcggcgg agggagctgg accccatgga cgtccgcggc
     7321 tccatcgtct acctggagat tgacaaccgg cagtgtgtgc aggcctcctc gcagtgcttc
     7381 cagagtgcca ccgacgtggc cgcattcctg ggagcgctcg cctcgctggg cagcctcaac
     7441 atcccctaca agatcgaggc cgtgcagagt gagaccgtgg agccgccccc gccggcgcag
     7501 ctgcacttca tgtacgtggc ggcggccgcc tttgtgcttc tgttcttcgt gggctgcggg
     7561 gtgctgctgt cccgcaagcg ccggcggcag catggccagc tctggttccc tgagggcttc
     7621 aaagtgtctg aggccagcaa gaagaagcgg cgggagcccc tcggcgagga ctccgtgggc
     7681 ctcaagcccc tgaagaacgc ttcagacggt gccctcatgg acgacaacca gaatgagtgg
     7741 ggggacgagg acctggagac caagaagttc cggttcgagg agcccgtggt tctgcctgac
     7801 ctggacgacc agacagacca ccggcagtgg actcagcagc acctggatgc cgctgacctg
     7861 cgcatgtctg ccatggcccc cacaccgccc cagggtgagg ttgacgccga ctgcatggac
     7921 gtcaatgtcc gcgggcctga tggcttcacc ccgctcatga tcgcctcctg cagcgggggc
     7981 ggcctggaga cgggcaacag cgaggaagag gaggacgcgc cggccgtcat ctccgacttc
     8041 atctaccagg gcgccagcct gcacaaccag acagaccgca cgggcgagac cgccttgcac
     8101 ctggccgccc gctactcacg ctctgatgcc gccaagcgcc tgctggaggc cagcgcagat
     8161 gccaacatcc aggacaacat gggccgcacc ccgctgcatg cggctgtgtc tgccgacgca
     8221 caaggtgtct tccagatcct gatccggaac cgagccacag acctggatgc ccgcatgcat
     8281 gatggcacga cgccactgat cctggctgcc cgcctggccg tggagggcat gctggaggac
     8341 ctcatcaact cacacgccga cgtcaacgcc gtagatgacc tgggcaagtc cgccctgcac
     8401 tgggccgccg ccgtgaacaa tgtggatgcc gcagttgtgc tcctgaagaa cggggctaac
     8461 aaagatatgc agaacaacag ggaggagaca cccctgtttc tggccgcccg ggagggcagc
     8521 tacgagaccg ccaaggtgct gctggaccac tttgccaacc gggacatcac ggatcatatg
     8581 gaccgcctgc cgcgcgacat cgcacaggag cgcatgcatc acgacatcgt gaggctgctg
     8641 gacgagtaca acctggtgcg cagcccgcag ctgcacggag ccccgctggg gggcacgccc
     8701 accctgtcgc ccccgctctg ctcgcccaac ggctacctgg gcagcctcaa gcccggcgtg
     8761 cagggcaaga aggtccgcaa gcccagcagc aaaggcctgg cctgtggaag caaggaggcc
     8821 aaggacctca aggcacggag gaagaagtcc caggacggca agggctgcct gctggacagc
     8881 tccggcatgc tctcgcccgt ggactccctg gagtcacccc atggctacct gtcagacgtg
     8941 gcctcgccgc cactgctgcc ctccccgttc cagcagtctc cgtccgtgcc cctcaaccac
     9001 ctgcctggga tgcccgacac ccacctgggc atcgggcacc tgaacgtggc ggccaagccc
     9061 gagatggcgg cgctgggtgg gggcggccgg ctggcctttg agactggccc acctcgtctc
     9121 tcccacctgc ctgtggcctc tggcaccagc accgtcctgg gctccagcag cggaggggcc
     9181 ctgaatttca ctgtgggcgg gtccaccagt ttgaatggtc aatgcgagtg gctgtcccgg
     9241 ctgcagagcg gcatggtgcc gaaccaatac aaccctctgc gggggagtgt ggcaccaggc
     9301 cccctgagca cacaggcccc ctccctgcag catggcatgg taggcccgct gcacagtagc
     9361 cttgctgcca gcgccctgtc ccagatgatg agctaccagg gcctgcccag cacccggctg
     9421 gccacccagc ctcacctggt gcagacccag caggtgcagc cacaaaactt acagatgcag
     9481 cagcagaacc tgcagccagc aaacatccag cagcagcaaa gcctgcagcc gccaccacca
     9541 ccaccacagc cgcaccttgg cgtgagctca gcagccagcg gccacctggg ccggagcttc
     9601 ctgagtggag agccgagcca ggcagacgtg cagccactgg gccccagcag cctggcggtg
     9661 cacactattc tgccccagga gagccccgcc ctgcccacgt cgctgccatc ctcgctggtc
     9721 ccacccgtga ccgcagccca gttcctgacg cccccctcgc agcacagcta ctcctcgcct
     9781 gtggacaaca cccccagcca ccagctacag gtgcctgagc accccttcct caccccgtcc
     9841 cctgagtccc ctgaccagtg gtccagctcg tccccgcatt ccaacgtctc cgactggtcc
     9901 gagggcgtct ccagccctcc caccagcatg cagtcccaga tcgcccgcat tccggaggcc
     9961 ttcaagtaag gatccgcggc cgcaaggatc tgcgatcgct ccggtgcccg tcagtgggca
    10021 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
    10081 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
    10141 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
    10201 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
    10261 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
    10321 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
    10381 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
    10441 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
    10501 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc
    10561 ggcctgatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta tccgctggaa
    10621 gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga
    10681 acaattgctt ttacagatgc acatatcgag gtggacatca cttacgctga gtacttcgaa
    10741 atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc
    10801 gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc
    10861 ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg
    10921 ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac
    10981 gtgcaaaaaa agctcccaat catccaaaaa attattatca tggattctaa aacggattac
    11041 cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa
    11101 tacgattttg tgccagagtc cttcgatagg gacaagacaa ttgcactgat catgaactcc
    11161 tctggatcta ctggtctgcc taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga
    11221 ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta
    11281 agtgttgttc cattccatca cggttttgga atgtttacta cactcggata tttgatatgt
    11341 ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttctgag gagccttcag
    11401 gattacaaga ttcaaagtgc gctgctggtg ccaaccctat tctccttctt cgccaaaagc
    11461 actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg tggcgctccc
    11521 ctctctaagg aagtcgggga agcggttgcc aagaggttcc atctgccagg tatcaggcaa
    11581 ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa
    11641 ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc
    11701 gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg
    11761 tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta
    11821 cattctggag acatagctta ctgggacgaa gacgaacact tcttcatcgt tgaccgcctg
    11881 aagtctctga ttaagtacaa aggctatcag gtggctcccg ctgaattgga atccatcttg
    11941 ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga tgacgccggt
    12001 gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc
    12061 gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt
    12121 gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc
    12181 ctcataaagg ccaagaaggg cggaaagatc gccgtgagat cccgcgctca gtcgtccaat
    12241 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt
    12301 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg
    12361 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
    12421 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
    12481 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
    12541 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
    12601 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
    12661 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
    12721 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
    12781 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
    12841 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
    12901 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa
    12961 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
    13021 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
    13081 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
    13141 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
    13201 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
    13261 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
    13321 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
    13381 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
    13441 cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt
    13501 cggatctccc tttggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca
    13561 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
    13621 caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
    13681 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    13741 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
    13801 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
    13861 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
    13921 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    13981 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
    14041 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
    14101 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
    14161 gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt
    14221 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    14281 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    14341 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    14401 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    14461 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    14521 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    14581 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    14641 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    14701 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    14761 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    14821 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    14881 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    14941 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    15001 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    15061 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    15121 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    15181 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    15241 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    15301 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    15361 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    15421 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    15481 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    15541 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    15601 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    15661 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    15721 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    15781 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    15841 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    15901 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    15961 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    16021 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    16081 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    16141 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    16201 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    16261 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    16321 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
    16381 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
    16441 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
    16501 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
    16561 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
    16621 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
    16681 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    16741 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    16801 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//
