LOCUS       V020895                12270 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020895
VERSION     V020895
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2291..2300
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2300..5392
                     /label="MRTFA(NM_020831)"
                     /note="MRTFA(NM_020831)"
                     /gene="MRTFA"
     promoter        5442..5653
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5666..5934
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5963..5972
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5993..7642
                     /EC_number=""
                     /codon_start=1
                     /gene="luc+"
                     /note="enhanced&nbsp;luc+ version of the luciferase gene"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label="luciferase"
     CDS             7712..7765
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7766..8365
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8366..8953
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9027..9260
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9332..9453
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9472..9607
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9645..9661)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9669..9685
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9693..9723)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9738..9759
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10047..10635)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10806..11666)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11667..11771)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12245..12261
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg tggatttctc cagtgtggtg tgcctgcccc cttccgtcat
     2341 tgctgtgaat gggctggacg gaggaggggc cggcgaaaat gatgatgaac cagtgctcgt
     2401 gtccttatct gcggcaccca gtccccagag tgaagctgtt gccaatgaac tgcaggagct
     2461 ctccttgcag cccgagctga ccctaggcct ccaccctggc aggaatccca atttgcctcc
     2521 acttagtgag cggaagaatg tgctacagtt gaaactccag cagcgccgga cccgggaaga
     2581 actggtgagc caagggatca tgccgccttt gaaaagtcca gccgcatttc atgagcagag
     2641 aaggagcttg gagcgggcca ggacagagga ctatctcaaa cggaagattc gttcccggcc
     2701 ggagagatcg gagctggtca ggatgcacat tttggaagag acctcggctg agccatccct
     2761 ccaggccaag cagctgaagc tgaagagagc cagactagcc gatgacctca atgagaagat
     2821 tgcacagagg cctggcccca tggagctggt ggagaagaac atccttcctg ttgagtccag
     2881 cctgaaggaa gccatcattg tgggccaggt gaactatccc aaagtagcag acagctcttc
     2941 cttcgatgag gacagcagcg atgccttatc ccccgagcag cctgccagcc atgagtccca
     3001 gggttctgtg ccgtcacccc tggaggcccg agtcagcgaa ccactgctca gtgccacctc
     3061 tgcatccccc acccaggttg tgtctcaact tccgatgggc cgggattcca gagaaatgct
     3121 tttcctggca gagcagcctc ctctgcctcc cccacctctg ctgcctccca gcctcaccaa
     3181 tggaaccact atccccactg ccaagtccac ccccacactc attaagcaaa gccaacccaa
     3241 gtctgccagt gagaagtcac agcgcagcaa gaaggccaag gagctgaagc caaaggtgaa
     3301 gaagctcaag taccaccagt acatcccccc ggaccagaag caggacaggg gggcaccccc
     3361 catggactca tcctacgcca agatcctgca gcagcagcag ctcttcctcc agctgcagat
     3421 cctcaaccag cagcagcagc agcaccacaa ctaccaggcc atcctgcctg ccccgccaaa
     3481 gtcagcaggc gaggccctgg gaagcagcgg gaccccccca gtacgcagcc tctccactac
     3541 caatagcagc tccagctcgg gcgcccctgg gccctgtggg ctggcacgtc agaacagcac
     3601 ctcactgact ggcaagccgg gagccctgcc ggccaacctg gacgacatga aggtggcaga
     3661 gctgaagcag gagctgaagt tgcgatcact gcctgtctcg ggcaccaaaa ctgagctgat
     3721 tgagcgcctt cgagcctatc aagaccaaat cagccctgtg ccaggagccc ccaaggcccc
     3781 tgccgccacc tctatcctgc acaaggctgg cgaggtggtg gtagccttcc cagcggcccg
     3841 gctgagcacg gggccagccc tggtggcagc aggcctggct ccagctgagg tggtggtggc
     3901 cacggtggcc agcagtgggg tggtgaagtt tggcagcacg ggctccacgc cccccgtgtc
     3961 tcccaccccc tcggagcgct cactgctcag cacgggcgat gaaaactcca cccccgggga
     4021 cacctttggt gagatggtga catcacctct gacgcagctg accctgcagg cctcgccact
     4081 gcagatcctc gtgaaggagg agggcccccg ggccgggtcc tgttgcctga gccctggggg
     4141 gcgggcggag ctagaggggc gcgacaagga ccagatgctg caggagaaag acaagcagat
     4201 cgaggcgctg acgcgcatgc tccggcagaa gcagcagctg gtggagcggc tcaagctgca
     4261 gctggagcag gagaagcgag cccagcagcc cgcccccgcc cccgcccccc tcggcacccc
     4321 cgtgaagcag gagaacagct tctccagctg ccagctgagc cagcagcccc tgggccccgc
     4381 tcacccattc aaccccagcc tggcggcccc agccaccaac cacatagacc cttgtgctgt
     4441 ggccccgggg cccccgtccg tggtggtgaa gcaggaagcc ttgcagcctg agcccgagcc
     4501 ggtccccgcc ccccagttgc ttctggggcc tcagggcccc agcctcatca agggggttgc
     4561 acctcccacc ctcatcaccg actccacagg gacccacctt gtcctcaccg tgaccaataa
     4621 gaatgcagac agccctggcc tgtccagtgg gagcccccag cagccctcgt cccagcctgg
     4681 ctctccagcg cctgccccct ctgcccagat ggacctggag cacccactgc agcccctctt
     4741 tgggaccccc acttctctgc tgaagaagga accacctggc tatgaggaag ccatgagcca
     4801 gcagcccaaa cagcaggaaa atggttcctc aagccagcag atggacgacc tgtttgacat
     4861 tctcattcag agcggagaaa tttcagcaga tttcaaggag ccgccatccc tgccagggaa
     4921 ggagaagcca tccccgaaga cagtctgtgg gtcccccctg gcagcacagc catcaccttc
     4981 tgctgagctc ccccaggctg ccccacctcc tccaggctca ccctccctcc ctggacgcct
     5041 ggaggacttc ctggagagca gcacggggct gcccctgctg accagtgggc atgacgggcc
     5101 agagcccctt tccctcattg acgacctcca tagccagatg ctgagcagca ctgccatcct
     5161 ggaccacccc ccgtcaccca tggacacctc ggaattgcac tttgttcctg agcccagcag
     5221 caccatgggc ctggacctgg ctgatggcca cctggacagc atggactggc tggagctgtc
     5281 gtcaggtggt cccgtgctga gcctagcccc cctcagcacc acagccccca gcctcttctc
     5341 cacagacttc ctcgatggcc atgatttgca gctgcactgg gattcctgct tgtagggatc
     5401 cgcggccgca aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc
     5461 cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg
     5521 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     5581 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     5641 cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc
     5701 tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc
     5761 ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg
     5821 tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct
     5881 gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct
     5941 gtgaccggcg cctacgctag acgccaccat ggagagcgac gagagcggcc tgatggaaga
     6001 cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg
     6061 agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac
     6121 agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt
     6181 ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga
     6241 aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc
     6301 gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc
     6361 taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct
     6421 cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc
     6481 gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc
     6541 agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg
     6601 tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag
     6661 agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt
     6721 ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt
     6781 cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca
     6841 aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa
     6901 atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt
     6961 cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac
     7021 tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg
     7081 taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg
     7141 cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa
     7201 caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat
     7261 agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa
     7321 gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa
     7381 catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc
     7441 cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc
     7501 cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc
     7561 gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa
     7621 gaagggcgga aagatcgccg tgagatcccg cgctcagtcg tccaattctg ccgtggacgg
     7681 caccgccgga cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg
     7741 tgacgtggag gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac
     7801 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     7861 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     7921 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     7981 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     8041 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     8101 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     8161 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     8221 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     8281 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     8341 catgacccgc aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt
     8401 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     8461 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     8521 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     8581 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     8641 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     8701 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     8761 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     8821 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     8881 ggctctgcgg cctcttccgc gtctccgcct tcgccctcag acgagtcgga tctccctttg
     8941 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     9001 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga
     9061 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9121 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9181 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9241 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     9301 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     9361 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     9421 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     9481 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     9541 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     9601 ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct
     9661 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     9721 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     9781 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     9841 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     9901 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9961 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    10021 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    10081 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    10141 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    10201 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    10261 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    10321 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    10381 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    10441 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
    10501 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    10561 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    10621 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    10681 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    10741 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    10801 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    10861 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    10921 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    10981 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    11041 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    11101 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    11161 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    11221 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    11281 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    11341 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    11401 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    11461 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    11521 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    11581 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    11641 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    11701 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    11761 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
    11821 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
    11881 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
    11941 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
    12001 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
    12061 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
    12121 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
    12181 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
    12241 cgttgtaaaa cgacggccag tgccaagctg
//
