LOCUS       V037060                12440 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037060
VERSION     V037060
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5469
                     /label="KIF11(NM_004523)"
                     /note="KIF11(NM_004523)"
                     /gene="KIF11"
     CDS             5476..5502
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5506..5532
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5536..5562
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5612..5823
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5836..6104
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6133..6142
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6163..7812
                     /EC_number=""
                     /codon_start=1
                     /gene="luc+"
                     /note="enhanced&nbsp;luc+ version of the luciferase gene"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label="luciferase"
     CDS             7882..7935
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7936..8535
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8536..9123
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9197..9430
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9502..9623
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9642..9777
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9815..9831)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9839..9855
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9863..9893)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9908..9929
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10217..10805)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10976..11836)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11837..11941)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12415..12431
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggcgtcg cagccaaatt cgtctgcgaa gaagaaagag
     2341 gagaagggga agaacatcca ggtggtggtg agatgcagac catttaattt ggcagagcgg
     2401 aaagctagcg cccattcaat agtagaatgt gatcctgtac gaaaagaagt tagtgtacga
     2461 actggaggat tggctgacaa gagctcaagg aaaacataca cttttgatat ggtgtttgga
     2521 gcatctacta aacagattga tgtttaccga agtgttgttt gtccaattct ggatgaagtt
     2581 attatgggct ataattgcac tatctttgcg tatggccaaa ctggcactgg aaaaactttt
     2641 acaatggaag gtgaaaggtc acctaatgaa gagtatacct gggaagagga tcccttggct
     2701 ggtataattc cacgtaccct tcatcaaatt tttgagaaac ttactgataa tggtactgaa
     2761 ttttcagtca aagtgtctct gttggagatc tataatgaag agctttttga tcttcttaat
     2821 ccatcatctg atgtttctga gagactacag atgtttgatg atccccgtaa caagagagga
     2881 gtgataatta aaggtttaga agaaattaca gtacacaaca aggatgaagt ctatcaaatt
     2941 ttagaaaagg gggcagcaaa aaggacaact gcagctactc tgatgaatgc atactctagt
     3001 cgttcccact cagttttctc tgttacaata catatgaaag aaactacgat tgatggagaa
     3061 gagcttgtta aaatcggaaa gttgaacttg gttgatcttg caggaagtga aaacattggc
     3121 cgttctggag ctgttgataa gagagctcgg gaagctggaa atataaatca atccctgttg
     3181 actttgggaa gggtcattac tgcccttgta gaaagaacac ctcatgttcc ttatcgagaa
     3241 tctaaactaa ctagaatcct ccaggattct cttggagggc gtacaagaac atctataatt
     3301 gcaacaattt ctcctgcatc tctcaatctt gaggaaactc tgagtacatt ggaatatgct
     3361 catagagcaa agaacatatt gaataagcct gaagtgaatc agaaactcac caaaaaagct
     3421 cttattaagg agtatacgga ggagatagaa cgtttaaaac gagatcttgc tgcagcccgt
     3481 gagaaaaatg gagtgtatat ttctgaagaa aattttagag tcatgagtgg aaaattaact
     3541 gttcaagaag agcagattgt agaattgatt gaaaaaattg gtgctgttga ggaggagctg
     3601 aatagggtta cagagttgtt tatggataat aaaaatgaac ttgaccagtg taaatctgac
     3661 ctgcaaaata aaacacaaga acttgaaacc actcaaaaac atttgcaaga aactaaatta
     3721 caacttgtta aagaagaata tatcacatca gctttggaaa gtactgagga gaaacttcat
     3781 gatgctgcca gcaagctgct taacacagtt gaagaaacta caaaagatgt atctggtctc
     3841 cattccaaac tggatcgtaa gaaggcagtt gaccaacaca atgcagaagc tcaggatatt
     3901 tttggcaaaa acctgaatag tctgtttaat aatatggaag aattaattaa ggatggcagc
     3961 tcaaagcaaa aggccatgct agaagtacat aagaccttat ttggtaatct gctgtcttcc
     4021 agtgtctctg cattagatac cattactaca gtagcacttg gatctctcac atctattcca
     4081 gaaaatgtgt ctactcatgt ttctcagatt tttaatatga tactaaaaga acaatcatta
     4141 gcagcagaaa gtaaaactgt actacaggaa ttgattaatg tactcaagac tgatcttcta
     4201 agttcactgg aaatgatttt atccccaact gtggtgtcta tactgaaaat caatagtcaa
     4261 ctaaagcata ttttcaagac ttcattgaca gtggccgata agatagaaga tcaaaaaaag
     4321 gaactagatg gctttctcag tatactgtgt aacaatctac atgaactaca agaaaatacc
     4381 atttgttcct tggttgagtc acaaaagcaa tgtggaaacc taactgaaga cctgaagaca
     4441 ataaagcaga cccattccca ggaactttgc aagttaatga atctttggac agagagattc
     4501 tgtgctttgg aggaaaagtg tgaaaatata cagaaaccac ttagtagtgt ccaggaaaat
     4561 atacagcaga aatctaagga tatagtcaac aaaatgactt ttcacagtca aaaattttgt
     4621 gctgattctg atggcttctc acaggaactc agaaatttta accaagaagg tacaaaattg
     4681 gttgaagaat ctgtgaaaca ctctgataaa ctcaatggca acctggaaaa aatatctcaa
     4741 gagactgaac agagatgtga atctctgaac acaagaacag tttatttttc tgaacagtgg
     4801 gtatcttcct taaatgaaag ggaacaggaa cttcacaact tattggaggt tgtaagccaa
     4861 tgttgtgagg cttcaagttc agacatcact gagaaatcag atggacgtaa ggcagctcat
     4921 gagaaacagc ataacatttt tcttgatcag atgactattg atgaagataa attgatagca
     4981 caaaatctag aacttaatga aaccataaaa attggtttga ctaagcttaa ttgctttctg
     5041 gaacaggatc tgaaactgga tatcccaaca ggtacgacac cacagaggaa aagttattta
     5101 tacccatcaa cactggtaag aactgaacca cgtgaacatc tccttgatca gctgaaaagg
     5161 aaacagcctg agctgttaat gatgctaaac tgttcagaaa acaacaaaga agagacaatt
     5221 ccggatgtgg atgtagaaga ggcagttctg gggcagtata ctgaagaacc tctaagtcaa
     5281 gagccatctg tagatgctgg tgtggattgt tcatcaattg gcggggttcc atttttccag
     5341 cataaaaaat cacatggaaa agacaaagaa aacagaggca ttaacacact ggagaggtct
     5401 aaagtggaag aaactacaga gcacttggtt acaaagagca gattacctct gcgagcccag
     5461 atcaaccttc tcgagtaccc atacgacgta cctgattatg caacttatcc ttatgatgta
     5521 cctgattatg ctagctatcc ttatgatgtc ccagactacg catagggatc cgcggccgca
     5581 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     5641 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     5701 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     5761 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     5821 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     5881 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     5941 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     6001 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     6061 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     6121 cctacgctag acgccaccat ggagagcgac gagagcggcc tgatggaaga cgccaaaaac
     6181 ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg agagcaactg
     6241 cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat
     6301 atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct
     6361 atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt
     6421 caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac
     6481 gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc taccgtggtg
     6541 ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc
     6601 caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg
     6661 ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc agagtccttc
     6721 gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg tctgcctaaa
     6781 ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag agatcctatt
     6841 tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt
     6901 tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat
     6961 agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca aagtgcgctg
     7021 ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa atacgattta
     7081 tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt cggggaagcg
     7141 gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac tgagactaca
     7201 tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt
     7261 ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcaa
     7321 agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa caatccggaa
     7381 gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg
     7441 gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa gtacaaaggc
     7501 tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa catcttcgac
     7561 gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt
     7621 ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta
     7681 acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt
     7741 accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga
     7801 aagatcgccg tgagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga
     7861 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag
     7921 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     7981 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     8041 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     8101 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     8161 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     8221 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     8281 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     8341 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     8401 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     8461 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     8521 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     8581 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     8641 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     8701 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     8761 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     8821 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     8881 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     8941 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     9001 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     9061 cctcttccgc gtctccgcct tcgccctcag acgagtcgga tctccctttg gccgcctccc
     9121 cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     9181 aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt
     9241 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     9301 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     9361 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     9421 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     9481 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     9541 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     9601 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     9661 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     9721 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     9781 acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     9841 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     9901 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     9961 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    10021 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    10081 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    10141 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    10201 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    10261 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    10321 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    10381 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    10441 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    10501 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    10561 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    10621 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
    10681 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    10741 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    10801 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    10861 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    10921 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    10981 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    11041 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    11101 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    11161 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    11221 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    11281 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    11341 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    11401 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    11461 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    11521 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    11581 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    11641 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    11701 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    11761 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    11821 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    11881 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    11941 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
    12001 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
    12061 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
    12121 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
    12181 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
    12241 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
    12301 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
    12361 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
    12421 cgacggccag tgccaagctg
//
