LOCUS       V039779                10756 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039779
VERSION     V039779
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4782
                     /label="Fgfr1(NM_010206)"
                     /note="Fgfr1(NM_010206)"
                     /gene="Fgfr1"
     CDS             4789..4854
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4898..5109
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5122..5390
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5419..5428
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5449..6111
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6181..6234
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6235..6834
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6835..7423
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7497..7730
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7802..7923
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7963..8098
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8131..8147)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8155..8171
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8179..8209)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8224..8245
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8533..9121)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9292..10152)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10153..10257)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10731..10747
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt ggggctggaa gtgcctcctc
     2341 ttctgggctg tgctggtcac agccactctc tgcactgcca ggccagcccc aaccttgcct
     2401 gaacaagctc agccctgggg agtccctgtg gaagtggagt ctctcctggt ccaccctggc
     2461 gacctgctac agcttcgctg tcggcttcgc gatgatgtgc agagcatcaa ctggctgcgg
     2521 gatggggtgc agctggtgga gagcaaccgt acccgcatca caggggagga ggtggaggtg
     2581 cgggactcca tccccgctga ctctggcctc tacgcttgcg tgaccagcag cccctctggc
     2641 agcgatacca cctacttctc cgtcaatgtc tcagatgcac tcccatcctc ggaagatgat
     2701 gacgacgacg atgactcctc ctcggaggag aaagagacgg acaacaccaa accaaaccgt
     2761 aggcctgtag ctccctactg gacatcccca gagaaaatgg agaagaaact gcatgcggtg
     2821 cccgctgcca agacggtgaa gttcaagtgc ccgtcgagtg ggacacccaa ccccactctg
     2881 cgctggttga aaaatggcaa agagtttaag cctgaccacc gaattggagg ctacaaggtt
     2941 cgctatgcca cctggagcat cataatggat tctgtggtgc cttctgacaa gggcaactac
     3001 acctgcatcg tggagaatga gtatgggagc atcaaccaca cctaccagct tgacgtcgtg
     3061 gaacgatctc cgcaccgacc catccttcag gcagggctgc ctgccaacaa gacagtggcc
     3121 ctgggcagca atgtggagtt catgtgtaag gtgtacagcg atccgcagcc tcacattcag
     3181 tggctgaagc acatcgaggt gaacgggagt aagatcgggc cagacaactt gccgtatgtc
     3241 cagatcctga agactgctgg agttaatacc accgacaagg aaatggaggt gcttcatcta
     3301 cggaatgtct cctttgagga tgcgggggag tatacgtgct tggcgggtaa ctctatcgga
     3361 ctctcccatc actctgcatg gttgaccgtt ctggaagccc tggaagagag accagctgtg
     3421 atgacctcac cgctctacct ggagatcatt atctactgca ccggggcctt cctgatctcc
     3481 tgcatgttgg gctctgtcat catctataag atgaagagcg gcaccaagaa gagcgacttc
     3541 catagccaga tggctgtgca caagctggcc aagagcatcc ctctgcgcag acaggtaaca
     3601 gtgtcagctg actccagtgc atccatgaac tctggggttc tcctggttcg gccctcacgg
     3661 ctctcctcca gcgggacccc catgctggct ggagtctccg aatatgagct ccctgaggat
     3721 ccccgctggg agctgccacg agacagactg gtcttaggca aaccacttgg cgagggctgc
     3781 ttcgggcagg tggtgttggc tgaggccatc gggctggata aggacaaacc caaccgtgtg
     3841 accaaagtgg ccgtgaagat gttgaagtcc gacgcaacgg agaaggacct gtcggatctg
     3901 atctcggaga tggagatgat gaaaatgatt gggaagcaca agaatatcat caaccttctg
     3961 ggagcgtgca cacaggatgg tcctctttat gtcattgtgg agtacgcctc caaaggcaat
     4021 ctccgggagt atctacaggc ccggaggcct cctgggctgg agtactgcta taaccccagc
     4081 cacaaccccg aggaacagct gtcttccaaa gatctggtat cctgtgccta tcaggtggct
     4141 cggggcatgg agtatcttgc ctctaagaag tgtatacacc gagacctggc tgctaggaac
     4201 gtcctggtga ccgaggataa cgtaatgaag atcgcagact ttggcttagc tcgagacatt
     4261 catcatatcg actactacaa gaaaaccacc aacggccggc tgcctgtgaa gtggatggcc
     4321 cctgaggcgt tgtttgaccg gatctacaca caccagagcg atgtgtggtc ttttggagtg
     4381 ctcttgtggg agatcttcac tctgggtggc tccccatacc ccggtgtgcc tgtggaggaa
     4441 cttttcaagc tgctgaagga gggtcatcga atggacaagc ccagtaactg taccaatgag
     4501 ctgtacatga tgatgcggga ctgctggcat gcagtgccct ctcagagacc tacgttcaag
     4561 cagttggtgg aagacctgga ccgcattgtg gccttgacct ccaaccagga gtatctggac
     4621 ctgtccatac cgctggacca gtactcaccc agctttcccg acacacggag ctccacctgc
     4681 tcctcagggg aggactctgt cttctctcat gagccgttac ctgaggagcc ctgtctgcct
     4741 cgacacccca cccagcttgc caacagtgga ctcaaacggc gcggatccga ctacaaagac
     4801 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagcgg
     4861 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     4921 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     4981 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     5041 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     5101 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     5161 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     5221 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     5281 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     5341 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     5401 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag
     5461 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga
     5521 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg
     5581 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc
     5641 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac
     5701 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac
     5761 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc
     5821 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg
     5881 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac
     5941 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg
     6001 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag
     6061 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc
     6121 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc
     6181 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     6241 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     6301 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     6361 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     6421 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     6481 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     6541 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6601 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6661 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6721 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6781 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa
     6841 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6901 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6961 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7021 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7081 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7141 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     7201 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     7261 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7321 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     7381 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc
     7441 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7501 agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg
     7561 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7621 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7681 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7741 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     7801 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7861 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7921 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     7981 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8041 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8101 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     8161 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8221 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8281 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8341 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8401 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8461 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8521 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8581 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8641 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8701 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8761 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8821 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8881 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8941 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     9001 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9061 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9121 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9181 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9241 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9301 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9361 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9421 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9481 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9541 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9601 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9661 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9721 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9781 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9841 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9901 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9961 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10021 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10081 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10141 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10201 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10261 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    10321 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
    10381 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    10441 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
    10501 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    10561 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
    10621 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    10681 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    10741 ggccagtgcc aagctg
//
