LOCUS       V044032                10616 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044032
VERSION     V044032
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..3018
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             3055..5541
                     /label="FYB1(NM_001465)"
                     /note="FYB1(NM_001465)"
                     /gene="FYB1"
     promoter        5586..5797
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5810..6078
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6105..6704
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6711..7299
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7373..7606
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7678..7799
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7818..7953
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7991..8007)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8015..8031
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8039..8069)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8084..8105
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8393..8981)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9152..10012)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10013..10117)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10591..10607
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     2341 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2401 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     2461 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     2521 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2581 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2641 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2701 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     2761 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     2821 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2881 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     2941 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3001 atggacgagc tgtacaaggg cggcggaggg agtggaggag ggggaagtgg atccatggcg
     3061 aaatataaca cggggggcaa cccgacagag gatgtctcag tcaatagccg acccttcaga
     3121 gtcacagggc caaactcatc ttcaggaata caagcaagaa agaacttatt caacaaccaa
     3181 ggaaatgcca gccctcctgc aggacccagc aatgtaccta agtttgggtc cccaaagcca
     3241 cctgtggcag tcaaaccttc ttctgaggaa aagcctgaca aggaacccaa gcccccgttt
     3301 ctaaagccca ctggagcagg ccaaagattc ggaacaccag ccagcttgac caccagagac
     3361 cccgaggcga aagtgggatt tctgaaacct gtaggcccca agcccatcaa cttgcccaaa
     3421 gaagattcca aacctacatt tccctggcct cctggaaaca agccatctct tcacagtgta
     3481 aaccaagacc atgacttaaa gccactaggc ccgaaatctg ggcctactcc tccaacctca
     3541 gaaaatgaac agaagcaagc gtttcccaaa ttgactgggg ttaaagggaa atttatgtca
     3601 gcatcacaag atcttgaacc caagcccctc ttccccaaac ccgcctttgg ccagaagccg
     3661 cccctaagta ccgagaactc ccatgaagac gaaagcccca tgaagaatgt gtcttcatca
     3721 aaagggtccc cagctcccct gggagtcagg tccaaaagcg gccctttaaa accagcaagg
     3781 gaagactcag aaaataaaga ccatgcaggg gagatttcaa gtttgccctt tcctggagtg
     3841 gttttgaaac ctgctgcgag caggggaggc ccaggtctct ccaaaaatgg tgaagaaaaa
     3901 aaggaagata ggaagataga tgctgctaag aacaccttcc agagcaaaat aaatcaggaa
     3961 gagttggcct cagggactcc tcctgccagg ttccctaagg ccccttctaa gctgacagtg
     4021 ggggggccat ggggccaaag tcaggaaaag gaaaagggag acaagaattc agccaccccg
     4081 aaacagaagc cattgcctcc cttgtttacc ttgggtccac ctccaccaaa acccaacaga
     4141 ccaccaaatg ttgacctgac gaaattccac aaaacctctt ctggaaacag tactagcaaa
     4201 ggccagacgt cttactcaac aacttccctg ccaccacctc caccatccca tccggccagc
     4261 caaccaccat tgccagcatc tcacccatca caaccaccag tcccaagcct acctcccaga
     4321 aacattaaac ctccgtttga cctaaaaagc cctgtcaatg aagacaatca agatggtgtc
     4381 acgcactctg atggtgctgg aaatctagat gaggaacaag acagtgaagg agaaacatat
     4441 gaagacatag aagcatccaa agaaagagag aagaaaaggg aaaaggaaga aaagaagagg
     4501 ttagagctgg agaaaaagga acagaaagag aaagaaaaga aagaacaaga aataaagaag
     4561 aaatttaaac taacaggccc tattcaagtc atccatcttg caaaagcttg ttgtgatgtc
     4621 aaaggaggaa agaatgaact gagcttcaag caaggagagc aaattgaaat catccgcatc
     4681 acagacaacc cagaaggaaa atggttgggc agaacagcaa ggggttcata tggctatatt
     4741 aaaacaactg ctgtagagat tgactatgat tctttgaaac tgaaaaaaga ctctcttggt
     4801 gccccttcaa gacctattga agatgaccaa gaagtatatg atgatgttgc agagcaggat
     4861 gatattagca gccacagtca gagtggaagt ggagggatat tccctccacc accagatgat
     4921 gacatttatg atgggattga agaggaagat gctgatgatg gctccacact acaggttcaa
     4981 gagaagagta atacgtggtc ctgggggatt ttgaagatgt taaagggaaa agatgacaga
     5041 aagaaaagta tacgagagaa acctaaagtc tctgactcag acaataatga aggttcatct
     5101 ttccctgctc ctcctaaaca attggacatg ggagatgaag tttacgatga tgtggatacc
     5161 tctgatttcc ctgtttcatc agcagagatg agtcaaggaa ctaatgttgg aaaagctaag
     5221 acagaagaaa aggaccttaa gaagctaaaa aagcaggaaa aagaagaaaa agacttcagg
     5281 aaaaaattta aatatgatgg tgaaattaga gtcctatatt caactaaagt tacaacttcc
     5341 ataacttcta aaaagtgggg aaccagagat ctacaggtaa aacctggtga atctctagaa
     5401 gttatacaaa ccacagatga cacaaaagtt ctctgcagaa atgaagaagg gaaatatggt
     5461 tatgtccttc ggagttacct agcggacaat gatggagaga tctatgatga tattgctgat
     5521 ggctgcatct atgacaatga ctaggcggcc gcgaaggatc tgcgatcgct ccggtgcccg
     5581 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     5641 ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     5701 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     5761 cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat
     5821 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt
     5881 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa
     5941 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg
     6001 gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt
     6061 tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctagatgacc gagtacaagc
     6121 ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg
     6181 cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg
     6241 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     6301 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     6361 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     6421 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     6481 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     6541 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     6601 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     6661 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagtcgac aatcaacctc
     6721 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6781 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6841 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6901 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6961 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     7021 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     7081 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     7141 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     7201 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     7261 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg
     7321 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     7381 ctaattcact cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta
     7441 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7501 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7561 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     7621 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     7681 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7741 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7801 catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7861 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     7921 tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt
     7981 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     8041 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     8101 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     8161 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     8221 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     8281 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     8341 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8401 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8461 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8521 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8581 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8641 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8701 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8761 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8821 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8881 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8941 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9001 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9061 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9121 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9181 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9241 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9301 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9361 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9421 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9481 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9541 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9601 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9661 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9721 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9781 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9841 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9901 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9961 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    10021 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    10081 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    10141 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
    10201 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
    10261 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
    10321 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
    10381 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
    10441 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
    10501 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
    10561 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
