LOCUS       V021679                10128 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021679
VERSION     V021679
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2285..4303
                     /label="FOXO3(NM_001455)"
                     /note="FOXO3(NM_001455)"
                     /gene="FOXO3"
     CDS             4337..5056
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     promoter        5098..5309
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5322..5590
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5617..6216
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6223..6811
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6885..7118
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7190..7311
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7330..7465
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7503..7519)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7527..7543
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7551..7581)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7596..7617
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7905..8493)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8664..9524)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9525..9629)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10103..10119
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 caccatggca gaggcaccgg cttccccggc cccgctctct ccgctcgaag tggagctgga
     2341 cccggagttc gagccccaga gccgtccgcg atcctgtacg tggcccctgc aaaggccgga
     2401 gctccaagcg agccctgcca agccctcggg ggagacggcc gccgactcca tgatccccga
     2461 ggaggaggac gatgaagacg acgaggacgg cgggggacgg gccggctcgg ccatggcgat
     2521 cggcggcggc ggcgggagcg gcacgctggg ctccgggctg ctccttgagg actcggcccg
     2581 ggtgctggca cccggagggc aagaccccgg gtctgggcca gccaccgcgg cgggcgggct
     2641 gagcgggggt acacaggcgc tgctgcagcc tcagcaaccg ctgccaccgc cgcagccggg
     2701 ggcggctggg ggctccgggc agccgaggaa atgttcgtcg cggcggaacg cctggggaaa
     2761 cctgtcctac gcggacctga tcacccgcgc catcgagagc tccccggaca aacggctcac
     2821 tctgtcccag atctacgagt ggatggtgcg ttgcgtgccc tacttcaagg ataagggcga
     2881 cagcaacagc tctgccggct ggaagaactc catccggcac aacctgtcac tgcatagtcg
     2941 attcatgcgg gtccagaatg agggaactgg caagagctct tggtggatca tcaaccctga
     3001 tggggggaag agcggaaaag ccccccggcg gcgggctgtc tccatggaca atagcaacaa
     3061 gtataccaag agccgtggcc gcgcagccaa gaagaaggca gccctgcaga cagcccccga
     3121 atcagctgac gacagtccct cccagctctc caagtggcct ggcagcccca cgtcacgcag
     3181 cagtgatgag ctggatgcgt ggacggactt ccgttcacgc accaattcta acgccagcac
     3241 agtcagtggc cgcctgtcgc ccatcatggc aagcacagag ttggatgaag tccaggacga
     3301 tgatgcgcct ctctcgccca tgctctacag cagctcagcc agcctgtcac cttcagtaag
     3361 caagccgtgc acggtggaac tgccacggct gactgatatg gcaggcacca tgaatctgaa
     3421 tgatgggctg actgaaaacc tcatggacga cctgctggat aacatcacgc tcccgccatc
     3481 ccagccatcg cccactgggg gactcatgca gcggagctct agcttcccgt ataccaccaa
     3541 gggctcgggc ctgggctccc caaccagctc ctttaacagc acggtgttcg gaccttcatc
     3601 tctgaactcc ctacgccagt ctcccatgca gaccatccaa gagaacaagc cagctacctt
     3661 ctcttccatg tcacactatg gtaaccagac actccaggac ctgctcactt cggactcact
     3721 tagccacagc gatgtcatga tgacacagtc ggaccccttg atgtctcagg ccagcaccgc
     3781 tgtgtctgcc cagaattccc gccggaacgt gatgcttcgc aatgatccga tgatgtcctt
     3841 tgctgcccag cctaaccagg gaagtttggt caatcagaac ttgctccacc accagcacca
     3901 aacccagggc gctcttggtg gcagccgtgc cttgtcgaat tctgtcagca acatgggctt
     3961 gagtgagtcc agcagccttg ggtcagccaa acaccagcag cagtctcctg tcagccagtc
     4021 tatgcaaacc ctctcggact ctctctcagg ctcctccttg tactcaacta gtgcaaacct
     4081 gcccgtcatg ggccatgaga agttccccag cgacttggac ctggacatgt tcaatgggag
     4141 cttggaatgt gacatggagt ccattatccg tagtgaactc atggatgctg atgggttgga
     4201 ttttaacttt gattccctca tctccacaca gaatgttgtt ggtttgaacg tggggaactt
     4261 cactggtgct aagcaggcct catctcagag ctgggtgcca ggctcgaatt taaatggatc
     4321 cggaggaggg ggaagcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     4381 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     4441 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     4501 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     4561 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     4621 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     4681 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     4741 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     4801 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     4861 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     4921 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     4981 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     5041 cgagctgtac aagtaagcgg ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg
     5101 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg
     5161 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     5221 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     5281 ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt
     5341 cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc
     5401 ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt
     5461 cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca
     5521 cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc
     5581 gttacagatc caagctgtga ccggcgccta cgctagatga ccgagtacaa gcccacggtg
     5641 cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc
     5701 gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag
     5761 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     5821 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     5881 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     5941 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     6001 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     6061 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     6121 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     6181 cgcacctggt gcatgacccg caagcccggt gcctgagtcg acaatcaacc tctggattac
     6241 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6301 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6361 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6421 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6481 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6541 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6601 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     6661 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     6721 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     6781 agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa
     6841 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     6901 ctcccaacga aaataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     6961 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7021 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7081 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     7141 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     7201 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7261 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7321 gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7381 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     7441 tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa ttcgtaatca
     7501 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     7561 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     7621 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7681 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7741 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7801 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7861 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7921 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7981 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8041 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8101 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     8161 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     8221 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8281 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     8341 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8401 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     8461 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     8521 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8581 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8641 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8701 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8761 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8821 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8881 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8941 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9001 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9061 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9121 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     9181 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     9241 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     9301 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     9361 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9421 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9481 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     9541 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9601 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     9661 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     9721 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     9781 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     9841 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     9901 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     9961 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
    10021 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
    10081 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
