LOCUS       V042406                10094 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042406
VERSION     V042406
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..3018
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             3055..5019
                     /label="FOXO1(NM_002015)"
                     /note="FOXO1(NM_002015)"
                     /gene="FOXO1"
     promoter        5064..5275
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5288..5556
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5583..6182
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6189..6777
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6851..7084
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7156..7277
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7296..7431
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7469..7485)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7493..7509
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7517..7547)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7562..7583
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7871..8459)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8630..9490)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9491..9595)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10069..10085
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     2341 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2401 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     2461 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     2521 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2581 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2641 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2701 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     2761 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     2821 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2881 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     2941 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3001 atggacgagc tgtacaaggg cggcggaggg agtggaggag ggggaagtgg atccatggcc
     3061 gaggcgcctc aggtggtgga gatcgacccg gacttcgagc cgctgccccg gccgcgctcg
     3121 tgcacctggc cgctgcccag gccggagttt agccagtcca actcggccac ctccagcccg
     3181 gcgccgtcgg gcagcgcggc tgccaacccc gacgccgcgg cgggcctgcc ctcggcctcg
     3241 gctgccgctg tcagcgccga cttcatgagc aacctgagct tgctggagga gagcgaggac
     3301 ttcccgcagg cgcccggctc cgtggcggcg gcggtggcgg cggcggccgc cgcggccgcc
     3361 accggggggc tgtgcgggga cttccagggc ccggaggcgg gctgcctgca cccagcgcca
     3421 ccgcagcccc cgccgcccgg gccgctgtcg cagcacccgc cggtgccccc cgccgccgct
     3481 gggccgctcg cggggcagcc gcgcaagagc agctcgtccc gccgcaacgc gtggggcaac
     3541 ctgtcctacg ccgacctcat caccaaggcc atcgagagct cggcggagaa gcggctcacg
     3601 ctgtcgcaga tctacgagtg gatggtcaag agcgtgccct acttcaagga taagggtgac
     3661 agcaacagct cggcgggctg gaagaattca attcgtcata atctgtccct acacagcaag
     3721 ttcattcgtg tgcagaatga aggaactgga aaaagttctt ggtggatgct caatccagag
     3781 ggtggcaaga gcgggaaatc tcctaggaga agagctgcat ccatggacaa caacagtaaa
     3841 tttgctaaga gccgaagccg agctgccaag aagaaagcat ctctccagtc tggccaggag
     3901 ggtgctgggg acagccctgg atcacagttt tccaaatggc ctgcaagccc tggctctcac
     3961 agcaatgatg actttgataa ctggagtaca tttcgccctc gaactagctc aaatgctagt
     4021 actattagtg ggagactctc acccattatg accgaacagg atgatcttgg agaaggggat
     4081 gtgcattcta tggtgtaccc gccatctgcc gcaaagatgg cctctacttt acccagtctg
     4141 tctgagataa gcaatcccga aaacatggaa aatcttttgg ataatctcaa ccttctctca
     4201 tcaccaacat cattaactgt ttcgacccag tcctcacctg gcaccatgat gcagcagacg
     4261 ccgtgctact cgtttgcgcc accaaacacc agtttgaatt cacccagccc aaactaccaa
     4321 aaatatacat atggccaatc cagcatgagc cctttgcccc agatgcctat acaaacactt
     4381 caggacaata agtcgagtta tggaggtatg agtcagtata actgtgcgcc tggactcttg
     4441 aaggagttgc tgacttctga ctctcctccc cataatgaca ttatgacacc agttgatcct
     4501 ggggtagccc agcccaacag ccgggttctg ggccagaacg tcatgatggg ccctaattcg
     4561 gtcatgtcaa cctatggcag ccaggcatct cataacaaaa tgatgaatcc cagctcccat
     4621 acccaccctg gacatgctca gcagacatct gcagttaacg ggcgtcccct gccccacacg
     4681 gtaagcacca tgccccacac ctcgggtatg aaccgcctga cccaagtgaa gacacctgta
     4741 caagtgcctc tgccccaccc catgcagatg agtgccctgg ggggctactc ctccgtgagc
     4801 agctgcaatg gctatggcag aatgggcctt ctccaccagg agaagctccc aagtgacttg
     4861 gatggcatgt tcattgagcg cttagactgt gacatggaat ccatcattcg gaatgacctc
     4921 atggatggag atacattgga ttttaacttt gacaatgtgt tgcccaacca aagcttccca
     4981 cacagtgtca agacaacgac acatagctgg gtgtcaggct gagcggccgc gaaggatctg
     5041 cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     5101 ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga
     5161 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     5221 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa
     5281 gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc
     5341 acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc
     5401 cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag
     5461 cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt
     5521 ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct
     5581 agatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg
     5641 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg
     5701 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     5761 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     5821 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     5881 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     5941 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     6001 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     6061 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     6121 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     6181 gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6241 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6301 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6361 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6421 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6481 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6541 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     6601 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6661 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6721 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     6781 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     6841 aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt
     6901 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6961 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7021 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7081 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7141 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7201 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7261 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt
     7321 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     7381 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt
     7441 gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7501 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7561 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7621 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7681 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7741 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7801 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7861 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7921 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7981 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8041 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8101 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8161 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8221 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8281 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     8341 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8401 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8461 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8521 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8581 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8641 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8701 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8761 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8821 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8881 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8941 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9001 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9061 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9121 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9181 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9241 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9301 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9361 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9421 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9481 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9541 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9601 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     9661 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     9721 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     9781 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     9841 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     9901 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     9961 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
    10021 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
    10081 ccagtgccaa gctg
//
