LOCUS       V039930                10375 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039930
VERSION     V039930
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..3018
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             3055..5229
                     /label="FGD3(NM_001083536)"
                     /note="FGD3(NM_001083536)"
                     /gene="FGD3"
     CDS             5236..5301
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5345..5556
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5569..5837
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5864..6463
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6470..7058
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7132..7365
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7437..7558
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7577..7712
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7750..7766)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7774..7790
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7798..7828)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7843..7864
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8152..8740)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8911..9771)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9772..9876)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10350..10366
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagattcta
     2281 gagctagcga attcagccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     2341 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2401 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     2461 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     2521 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2581 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2641 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2701 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     2761 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     2821 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2881 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     2941 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3001 atggacgagc tgtacaaggg aggaggggga agcggtggag gcggttctct cgagatggag
     3061 tcaggcaggg ggtcctcaac ccctccagga cccattgctg ccctagggat gccagacact
     3121 gggcctggca gttcctccct agggaagctt caggcgctcc ctgttgggcc cagagcccac
     3181 tgtggggacc ctgtcagcct ggctgcagca ggggacggct ctccagacat aggccccacg
     3241 ggagagctga gtggtagctt aaagatcccc aaccgggaca gcgggatcga cagtccctcc
     3301 tccagtgtgg ctggagagaa ctttccctgc gaggagggct tggaggctgg cccaagcccc
     3361 actgtactgg gggcgcacgc agagatggcc ctggacagcc aggtcccgaa ggtcaccccc
     3421 caggaggagg cggacagcga cgtgggtgag gaacctgact ctgagaacac cccccagaag
     3481 gctgacaagg atgccggcct ggcccagcac tctggccccc agaagcttct ccacattgcc
     3541 caggagctcc tgcacaccga ggagacctat gtgaagcggc tgcacctgct ggaccaggtt
     3601 ttctgcacca ggctgacgga tgcggggatc cctccagaag tcatcatggg catattctct
     3661 aacatctcct ccatccaccg cttccacggg cagttcctgc tgccggagct gaagacgcgg
     3721 atcacggagg agtgggacac aaacccacgg ctcggggaca tcctgcagaa gctggcccca
     3781 ttcctgaaga tgtacggcga gtatgtcaag aactttgacc gagccgtagg gctggtgagc
     3841 acgtggaccc agcgctcccc actgtttaaa gacgtcgtcc acagcatcca gaagcaggag
     3901 gtatgcggga acctgacgct gcagcaccac atgctggagc ccgtgcagag ggtcccccgg
     3961 tacgagctgc tgctcaagga ctatctgaag aggctcccgc aggacgcccc agaccggaag
     4021 gatgcggaga ggtccttgga gctcatctcc acagccgcca accactccaa tgctgccatt
     4081 cggaaagtgg agaaaatgca caagctcttg gaggtgtacg agcagctggg tggggaagaa
     4141 gacattgtca acccggccaa tgaactgatc aaggagggcc aaatccagaa actgtcagcc
     4201 aagaacggca ccccccagga ccgccacctc ttcctgttca acagcatgat cctttactgt
     4261 gtgcccaagc tgcggctcat gggccagaag ttcagcgtcc gggagaagat ggacatctca
     4321 ggcctccagg tgcaggatat cgtcaagcca aacacagcac atacattcat cataacagga
     4381 agaaaaaggt ccctggagct gcagacgcgg acagaggaag agaagaaaga atggattcag
     4441 atcatccagg ccaccatcga gaagcacaaa cagaacagcg aaaccttcaa ggcttttggt
     4501 ggcgccttca gccaggatga ggaccccagc ctctctccag acatgcctat cacgagcacc
     4561 agccctgtgg agcctgtggt gaccaccgaa ggcagttcgg gtgcagcagg gctcgagccc
     4621 agaaaactat cctctaagac cagacgtgac aaggagaagc agagctgtaa gagctgtggt
     4681 gagaccttca actccatcac caagaggagg catcactgca agctgtgtgg ggcggtcatc
     4741 tgtgggaagt gctccgagtt caaggccgag aacagccggc agagccgtgt ctgcagagat
     4801 tgtttcctga cacagccagt ggcccctgag agcacagaga agacacccac tgcagacccc
     4861 cagcccagcc tgctctgcgg ccccctgcgg ctgtcagaga gcggtgagac ctggagcgag
     4921 gtgtgggccg ccatccccat gtcagatccc caggtgctgc acctgcaggg aggcagccag
     4981 gacggccggc tgccccgcac catccctctc cccagctgca aactgagtgt gccggaccct
     5041 gaggagaggc tggactcggg gcatgtgtgg aagctgcagt gggccaagca gtcctggtac
     5101 ctgagcgcct cctccgcaga gctgcagcag cagtggctgg aaaccctaag cactgctgcc
     5161 catggggaca cggcccagga cagcccgggg gccctgcagc ttcaggtccc tatgggcgca
     5221 gctgctccgg gatccgacta caaagaccat gacggtgatt ataaagatca tgacatcgac
     5281 tacaaggatg acgatgacaa gtagcggccg cgaaggatct gcgatcgctc cggtgcccgt
     5341 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     5401 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     5461 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     5521 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     5581 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     5641 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     5701 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     5761 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     5821 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagatgaccg agtacaagcc
     5881 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc
     5941 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt
     6001 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     6061 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     6121 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     6181 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     6241 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     6301 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     6361 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     6421 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgaca atcaacctct
     6481 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6541 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6601 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6661 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6721 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6781 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6841 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     6901 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6961 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     7021 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga
     7081 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     7141 taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct ctctggttag
     7201 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7261 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7321 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     7381 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     7441 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7501 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7561 atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7621 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     7681 ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg gcccaaattc
     7741 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7801 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7861 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7921 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7981 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8041 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8101 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8161 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8221 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8281 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8341 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8401 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8461 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8521 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8581 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8641 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8701 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8761 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8821 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8881 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8941 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9001 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9061 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9121 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9181 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9241 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9301 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9361 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9421 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9481 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9541 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9601 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9661 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9721 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9781 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9841 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9901 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     9961 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    10021 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    10081 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
    10141 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
    10201 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    10261 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
    10321 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//
