LOCUS       V041730                11071 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041730
VERSION     V041730
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5097
                     /label="FBXO11(NM_001190274)"
                     /note="FBXO11(NM_001190274)"
                     /gene="FBXO11"
     CDS             5104..5169
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5213..5424
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5437..5705
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5734..5743
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5764..6426
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6496..6549
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6550..7149
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7150..7738
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7812..8045
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8117..8238
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8278..8413
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8446..8462)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8470..8486
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8494..8524)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8539..8560
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8848..9436)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9607..10467)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10468..10572)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11046..11062
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga actccgtccg agccgccaac
     2341 cggagaccca ggcgagtgtc gcggccgcgc ccggtgcagc aacagcagca gcagcccccg
     2401 cagcagccgc cgccgcagcc gccccagcag cagccgcccc agcagcagcc tccgccgccg
     2461 ccgcagcagc agcagcagca gcagcctccg ccgccgccac cgccgcctcc gccgctgcct
     2521 caggagcgga acaacgtcgg cgagcgggat gatgatgtgc ctgcagatat ggttgcagaa
     2581 gaatcaggtc ctggtgcaca aaatagtcca taccaacttc gtagaaaaac tcttttgccg
     2641 aaaagaacag cgtgtcccac aaagaacagt atggagggcg cctcaacttc aactacagaa
     2701 aactttggtc atcgtgcaaa acgtgcaaga gtgtctggaa aatcacaaga tctatcagca
     2761 gcacctgctg aacagtatct tcaggagaaa ctgccagatg aagtggttct aaaaatcttc
     2821 tcttacttgc tggaacagga tctttgtaga gcagcttgtg tatgtaaacg cttcagtgaa
     2881 cttgctaatg atccaatttt gtggaaacga ttatatatgg aagtatttga atatactcgc
     2941 cctatgatgc atcctgaacc tggaaaattc taccagatta atccagaaga gtatgaacat
     3001 ccaaatccct ggaaagagag tttccagcag ttgtataaag gtgcacatgt aaagccagga
     3061 tttgctgaac atttctacag taaccctgca agatataaag gaagagaaaa tatgttgtat
     3121 tatgatacta ttgaagatgc ccttggtggg gtacaagagg ctcattttga tggacttatc
     3181 tttgttcatt ctggaatata tactgatgaa tggatatata ttgaatctcc aatcaccatg
     3241 attggtgcag cacctgggaa agtggcagac aaagttataa ttgaaaacac tagagattca
     3301 accttcgttt ttatggaagg ctctgaagat gcttatgttg gatatatgac aataaggttt
     3361 aaccctgatg acaaatctgc acaacaccac aatgcacacc actgcttaga gattacagta
     3421 aattgtagcc ctattattga tcactgtatc atccgaagta catgtacagt tggttctgca
     3481 gtatgtgtta gtggtcaagg agcatgtccc accatcaagc actgtaacat cagtgactgt
     3541 gaaaatgttg gactatatat aacagatcat gcacagggaa tatatgagga taatgaaatt
     3601 tccaataatg cgttagctgg gatttgggtt aaaaatcatg gaaacccaat tattagacgg
     3661 aatcatattc atcatggacg tgatgttggt gtgttcacat ttgatcatgg catgggttac
     3721 tttgaaagtt gcaatataca cagaaatagg atagcaggct ttgaagtaaa agcctatgct
     3781 aaccctacag tggttcgatg tgaaattcac catgggcaga ctggaggaat atatgtccat
     3841 gaaaaaggaa gaggacaatt catagagaat aaaatctatg caaacaactt tgcaggtgta
     3901 tggattacct caaatagtga cccaacaata aggggaaatt ctatatttaa tggaaatcaa
     3961 ggaggagttt acatctttgg tgatggacga ggccttattg aaggaaatga catttatggc
     4021 aatgcattag caggaattca aattaggaca aacagttgtc caattgttcg gcataacaaa
     4081 attcatgatg gccagcatgg tgggatttat gtgcatgaaa agggacaagg agtaatagaa
     4141 gagaatgaag tttatagtaa cactctagct ggagtctggg tgacaactgg cagcactcca
     4201 gtactgagaa gaaaccggat acacagtggc aagcaggttg gtgtttattt ttatgacaat
     4261 ggacatggag tgctagaaga caatgatatc tataatcata tgtattcagg ggttcagata
     4321 aggactggaa gcaaccccaa aattagacgc aacaaaatct ggggaggaca gaatggtgga
     4381 attctagttt ataattctgg tctaggctgt atagaagaca atgaaatatt tgacaatgca
     4441 atggctggag tctggattaa gacagatagt aatcctacac taagaagaaa taaaatccat
     4501 gatggaagag atggtggcat ctgtatattt aatgggggtc gaggtctcct tgaagaaaat
     4561 gatattttca ggaatgctca agcaggtgtt ctcatcagca ctaatagtca tccaatctta
     4621 aggaaaaaca gaatatttga tggatttgcc gcaggtattg aaattacaaa tcacgcaact
     4681 gcaacactag aaggcaatca gatttttaac aaccggtttg gaggcttatt tttagcatct
     4741 ggtgttaatg tgacaatgaa agataacaaa ataatgaaca atcaagatgc catagaaaag
     4801 gctgttagta gaggccaatg tttatataaa atatcaagtt ataccagcta tcccatgcat
     4861 gatttctaca gatgtcatac ttgtaacacc acagatcgaa atgccatatg tgtgaactgc
     4921 attaagaagt gccatcaggg acatgatgta gagtttatta gacatgatag gtttttctgt
     4981 gactgtggtg ctggaacact gtctaatcct tgtacattag ctggtgagcc tacacatgat
     5041 acagatacac tatatgactc tgctccacct atagaatcta atacattgca gcacaacgga
     5101 tccgactaca aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac
     5161 gatgacaagt agcggccgcg aaggatctgc gatcgctccg gtgcccgtca gtgggcagag
     5221 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
     5281 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     5341 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     5401 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
     5461 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
     5521 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
     5581 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
     5641 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
     5701 agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc
     5761 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag
     5821 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc
     5881 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc
     5941 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac
     6001 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc
     6061 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc
     6121 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag
     6181 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg
     6241 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc
     6301 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg
     6361 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc
     6421 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc
     6481 tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
     6541 cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     6601 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     6661 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     6721 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     6781 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     6841 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     6901 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     6961 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     7021 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     7081 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     7141 ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     7201 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     7261 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     7321 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     7381 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     7441 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     7501 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     7561 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     7621 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     7681 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     7741 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7801 aaagggggga ctggaagggc taattcactc ccaacgaaga taagatctgc tttttgcttg
     7861 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7921 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7981 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8041 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     8101 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     8161 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     8221 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     8281 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8341 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     8401 ttttttggag gcctagactt ttgcagagac caaattcgta atcatgtcat agctgtttcc
     8461 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8521 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8581 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     8641 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8701 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8761 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8821 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8881 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8941 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     9001 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     9061 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     9121 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     9181 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     9241 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     9301 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     9361 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     9421 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9481 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9541 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9601 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9661 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9721 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9781 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9841 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9901 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9961 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    10021 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    10081 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    10141 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    10201 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    10261 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    10321 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    10381 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    10441 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    10501 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    10561 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
    10621 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
    10681 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
    10741 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
    10801 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
    10861 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
    10921 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
    10981 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
    11041 acgttgtaaa acgacggcca gtgccaagct g
//
