LOCUS       V025275                15848 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025275
VERSION     V025275
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..9849
                     /label="FASN(NM_004104)"
                     /note="FASN(NM_004104)"
                     /gene="FASN"
     CDS             9856..9882
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             9886..9912
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             9916..9942
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        10007..10218
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             10231..10499
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      10528..10537
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             10558..11220
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             11290..11343
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             11344..11943
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    11944..12531
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             12605..12838
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    12910..13031
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      13050..13185
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(13223..13239)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    13247..13263
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(13271..13301)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    13316..13337
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(13625..14213)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(14384..15244)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(15245..15349)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     15823..15839
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg aggaggtggt gattgccggc
     2341 atgtccggga agctgccaga gtcggagaac ttgcaggagt tctgggacaa cctcatcggc
     2401 ggtgtggaca tggtcacgga cgatgaccgt cgctggaagg cggggctcta cggcctgccc
     2461 cggcggtccg gcaagctgaa ggacctgtct aggtttgatg cctccttctt cggagtccac
     2521 cccaagcagg cacacacgat ggaccctcag ctgcggctgc tgctggaagt cacctatgaa
     2581 gccatcgtgg acggaggcat caacccagat tcactccgag gaacacacac tggcgtctgg
     2641 gtgggcgtga gcggctctga gacctcggag gccctgagcc gagaccccga gacactcgtg
     2701 ggctacagca tggtgggctg ccagcgagcg atgatggcca accggctctc cttcttcttc
     2761 gacttcagag ggcccagcat cgcactggac acagcctgct cctccagcct gatggccctg
     2821 cagaacgcct accaggccat ccacagcggg cagtgccctg ccgccatcgt ggggggcatc
     2881 aatgtcctgc tgaagcccaa cacctccgtg cagttcttga ggctggggat gctcagcccc
     2941 gagggcacct gcaaggcctt cgacacagcg gggaatgggt actgccgctc ggagggtgtg
     3001 gtggccgtcc tgctgaccaa gaagtccctg gcccggcggg tgtacgccac catcctgaac
     3061 gccggcacca atacagatgg cttcaaggag caaggcgtga ccttcccctc aggggatatc
     3121 caggagcagc tcatccgctc gttgtaccag tcggccggag tggcccctga gtcatttgaa
     3181 tacatcgaag cccacggcac aggcaccaag gtgggcgacc cccaggagct gaatggcatc
     3241 acccgagccc tgtgcgccac ccgccaggag ccgctgctca tcggctccac caagtccaac
     3301 atggggcacc cggagccagc ctcggggctg gcagccctgg ccaaggtgct gctgtccctg
     3361 gagcacgggc tctgggcccc caacctgcac ttccatagcc ccaaccctga gatcccagcg
     3421 ctgttggatg ggcggctgca ggtggtggac cagcccctgc ccgtccgtgg cggcaacgtg
     3481 ggcatcaact cctttggctt cgggggctcc aacgtgcaca tcatcctgag gcccaacacg
     3541 cagccgcccc ccgcacccgc cccacatgcc accctgcccc gtctgctgcg ggccagcgga
     3601 cgcacccctg aggccgtgca gaagctgctg gagcagggcc tccggcacag ccaggacctg
     3661 gctttcctga gcatgctgaa cgacatcgcg gctgtccccg ccaccgccat gcccttccgt
     3721 ggctacgctg tgctgggtgg tgagcgcggt ggcccagagg tgcagcaggt gcccgctggc
     3781 gagcgcccgc tctggttcat ctgctctggg atgggcacac agtggcgcgg gatggggctg
     3841 agcctcatgc gcctggaccg cttccgagat tccatcctac gctccgatga ggctgtgaag
     3901 ccattcggcc tgaaggtgtc acagctgctg ctgagcacag acgagagcac ctttgatgac
     3961 atcgtccatt cgtttgtgag cctgactgcc atccagatag gcctcataga cctgctgagc
     4021 tgcatggggc tgaggccaga tggcatcgtc ggccactccc tgggggaggt ggcctgtggc
     4081 tacgccgacg gctgcctgtc ccaggaggag gccgtcctcg ctgcctactg gaggggacag
     4141 tgcatcaaag aagcccatct cccgccgggc gccatggcag ccgtgggctt gtcctgggag
     4201 gagtgtaaac agcgctgccc cccgggcgtg gtgcccgcct gccacaactc caaggacaca
     4261 gtcaccatct cgggacctca ggccccggtg tttgagttcg tggagcagct gaggaaggag
     4321 ggtgtgtttg ccaaggaggt gcggaccggc ggtatggcct tccactccta cttcatggag
     4381 gccatcgcac ccccactgct gcaggagctc aagaaggtga tccgggagcc gaagccacgt
     4441 tcagcccgct ggctcagcac ctctatcccc gaggcccagt ggcacagcag cctggcacgc
     4501 acgtcctccg ccgagtacaa tgtcaacaac ctggtgagcc ctgtgctgtt ccaggaggcc
     4561 ctgtggcacg tgcctgagca cgcggtggtg ctggagatcg cgccccacgc cctgctgcag
     4621 gctgtcctga agcgtggcct gaagccgagc tgcaccatca tccccctgat gaagaaggat
     4681 cacagggaca acctggagtt cttcctggcc ggcatcggca ggctgcacct ctcaggcatc
     4741 gacgccaacc ccaatgcctt gttcccacct gtggagttcc cagctccccg aggaactccc
     4801 ctcatctccc cactcatcaa gtgggaccac agcctggcct gggacgtgcc ggccgccgag
     4861 gacttcccca acggttcagg ttccccctca gccgccatct acaacatcga caccagctcc
     4921 gagtctcctg accactacct ggtggaccac accctcgacg gtcgcgtcct cttccccgcc
     4981 actggctacc tgagcatagt gtggaagacg ctggcccgcg ccctgggcct gggcgtcgag
     5041 cagctgcctg tggtgtttga ggatgtggtg ctgcaccagg ccaccatcct gcccaagact
     5101 gggacagtgt ccctggaggt acggctcctg gaggcctccc gtgccttcga ggtgtcagag
     5161 aacggcaacc tggtagtgag tgggaaggtg taccagtggg atgaccctga ccccaggctc
     5221 ttcgaccacc cggaaagccc cacccccaac cccacggagc ccctcttcct ggcccaggct
     5281 gaagtttaca aggagctgcg tctgcgtggc tacgactacg gccctcattt ccagggcatc
     5341 ctggaggcca gcctggaagg tgactcgggg aggctgctgt ggaaggataa ctgggtgagc
     5401 ttcatggaca ccatgctgca gatgtccatc ctgggctcgg ccaagcacgg cctgtacctg
     5461 cccacccgtg tcaccgccat ccacatcgac cctgccaccc acaggcagaa gctgtacaca
     5521 ctgcaggaca aggcccaagt ggctgacgtg gtggtgagca ggtggctgag ggtcacagtg
     5581 gccggaggcg tccacatctc cgggctccac actgagtcgg ccccgcggcg gcagcaggag
     5641 cagcaggtgc ccatcctgga gaagttttgc ttcactcccc acacggagga ggggtgcctg
     5701 tctgagcgcg ctgccctgca ggaggagctg caactgtgca aggggctggt gcaggcactg
     5761 cagaccaagg tgacccagca ggggctgaag atggtggtgc ccggactgga tggggcccag
     5821 atcccccggg acccctcaca gcaggaactg ccccggctgt tgtcggctgc ctgcaggctt
     5881 cagctcaacg ggaacctgca gctggagctg gcgcaggtgc tggcccagga gaggcccaag
     5941 ctgccagagg accctctgct cagcggcctc ctggactccc cggcactcaa ggcctgcctg
     6001 gacactgccg tggagaacat gcccagcctg aagatgaagg tggtggaggt gctggctggc
     6061 cacggtcacc tgtattcccg catcccaggc ctgctcagcc cccatcccct gctgcagctg
     6121 agctacacgg ccaccgaccg ccacccccag gccctggagg ctgcccaggc cgagctgcag
     6181 cagcacgacg ttgcccaggg ccagtgggat cccgcagacc ctgcccccag cgccctgggc
     6241 agcgccgacc tcctggtgtg caactgtgct gtggctgccc tcggggaccc ggcctcagct
     6301 ctcagcaaca tggtggctgc cctgagagaa gggggctttc tgctcctgca cacactgctc
     6361 cgggggcacc ccctcgggga catcgtggcc ttcctcacct ccactgagcc gcagtatggc
     6421 cagggcatcc tgagccagga cgcgtgggag agcctcttct ccagggtgtc gctgcgcctg
     6481 gtgggcctga agaagtcctt ctacggctcc acgctcttcc tgtgccgccg gcccaccccg
     6541 caggacagcc ccatcttcct gccggtggac gataccagct tccgctgggt ggagtctctg
     6601 aagggcatcc tggctgacga agactcttcc cggcctgtgt ggctgaaggc catcaactgt
     6661 gccacctcgg gcgtggtggg cttggtgaac tgtctccgcc gagagcccgg cgggaaccgc
     6721 ctccggtgtg tgctgctctc caacctcagc agcacctccc acgtcccgga ggtggacccg
     6781 ggctccgcag aactgcagaa ggtgttgcag ggagacctgg tgatgaacgt ctaccgcgac
     6841 ggggcctggg gggctttccg ccacttcctg ctggaggagg acaagcctga ggagccgacg
     6901 gcacatgcct ttgtgagcac cctcacccgg ggggacctgt cctccatccg ctgggtctgc
     6961 tcctcgctgc gccatgccca gcccacctgc cctggcgccc agctctgcac ggtctactac
     7021 gcctccctca acttccgcga catcatgctg gccactggca agctgtcccc tgatgccatc
     7081 ccagggaagt ggacctccca ggacagcctg ctaggtatgg agttctcggg ccgagacgcc
     7141 agcggcaagc gtgtgatggg actggtgcct gccaagggcc tggccacctc tgtcctgctg
     7201 tcaccggact tcctctggga tgtgccttcc aactggacgc tggaggaggc ggcctcggtg
     7261 cctgtcgtct acagcacggc ctactacgcg ctggtggtgc gtgggcgggt gcgccccggg
     7321 gagacgctgc tcatccactc gggctcgggc ggcgtgggcc aggccgccat cgccatcgcc
     7381 ctcagtctgg gctgccgcgt cttcaccacc gtggggtcgg ctgagaagcg ggcgtacctc
     7441 caggccaggt tcccccagct cgacagcacc agcttcgcca actcccggga cacatccttc
     7501 gagcagcatg tgctgtggca cacgggcggg aagggcgttg acctggtctt gaactccttg
     7561 gcggaagaga agctgcaggc cagcgtgagg tgcttggcta cgcacggtcg cttcctggaa
     7621 attggcaaat tcgacctttc tcagaaccac ccgctcggca tggctatctt cctgaagaac
     7681 gtgacattcc acggggtcct actggatgcg ttcttcaacg agagcagtgc tgactggcgg
     7741 gaggtgtggg cgcttgtgca ggccggcatc cgggatgggg tggtacggcc cctcaagtgc
     7801 acggtgttcc atggggccca ggtggaggac gccttccgct acatggccca agggaagcac
     7861 attggcaaag tcgtcgtgca ggtgcttgcg gaggagccgg aggcagtgct gaagggggcc
     7921 aaacccaagc tgatgtcggc catctccaag accttctgcc cggcccacaa gagctacatc
     7981 atcgctggtg gtctgggtgg cttcggcctg gagttggcgc agtggctgat acagcgtggg
     8041 gtgcagaagc tcgtgttgac ttctcgctcc gggatccgga caggctacca ggccaagcag
     8101 gtccgccggt ggaggcgcca gggcgtacag gtgcaggtgt ccaccagcaa catcagctca
     8161 ctggaggggg cccggggcct cattgccgag gcggcgcagc ttgggcccgt gggcggcgtc
     8221 ttcaacctgg ccgtggtctt gagagatggc ttgctggaga accagacccc agagttcttc
     8281 caggacgtct gcaagcccaa gtacagcggc accctgaacc tggacagggt gacccgagag
     8341 gcgtgccctg agctggacta ctttgtggtc ttctcctctg tgagctgcgg gcgtggcaat
     8401 gcgggacaga gcaactacgg ctttgccaat tccgccatgg agcgtatctg tgagaaacgc
     8461 cggcacgaag gcctcccagg cctggccgtg cagtggggcg ccatcggcga cgtgggcatt
     8521 ttggtggaga cgatgagcac caacgacacg atcgtcagtg gcacgctgcc ccagcgcatg
     8581 gcgtcctgcc tggaggtgct ggacctcttc ctgaaccagc cccacatggt cctgagcagc
     8641 tttgtgctgg ctgagaaggc tgcggcctat agggacaggg acagccagcg ggacctggtg
     8701 gaggccgtgg cacacatcct gggcatccgc gacttggctg ctgtcaacct ggacagctca
     8761 ctggcggacc tgggcctgga ctcgctcatg agcgtggagg tgcgccagac gctggagcgt
     8821 gagctcaacc tggtgctgtc cgtgcgcgag gtgcggcaac tcacgctccg gaaactgcag
     8881 gagctgtcct caaaggcgga tgaggccagc gagctggcat gccccacgcc caaggaggat
     8941 ggtctggccc agcagcagac tcagctgaac ctgcgctccc tgctggtgaa cccggagggc
     9001 cccaccctga tgcggctcaa ctccgtgcag agctcggagc ggcccctgtt cctggtgcac
     9061 ccaatcgagg gctccaccac cgtgttccac agcctggcct cccggctcag catccccacc
     9121 tatggcctgc agtgcacccg agctgcgccc cttgacagca tccacagcct ggctgcctac
     9181 tacatcgact gcatcaggca ggtgcagccc gagggcccct accgcgtggc cggctactcc
     9241 tacggggcct gcgtggcctt tgaaatgtgc tcccagctgc aggcccagca gagcccagcc
     9301 cccacccaca acagcctctt cctgttcgac ggctcgccca cctacgtact ggcctacacc
     9361 cagagctacc gggcaaagct gaccccaggc tgtgaggctg aggctgagac ggaggccata
     9421 tgcttcttcg tgcagcagtt cacggacatg gagcacaaca gggtgctgga ggcgctgctg
     9481 ccgctgaagg gcctagagga gcgtgtggca gccgccgtgg acctgatcat caagagccac
     9541 cagggcctgg accgccagga gctgagcttt gcggcccggt ccttctacta caagctgcgt
     9601 gccgctgagc agtacacacc caaggccaag taccatggca acgtgatgct actgcgcgcc
     9661 aagacgggtg gcgcctacgg cgaggacctg ggcgcggact acaacctctc ccaggtatgc
     9721 gacgggaaag tatccgtcca cgtcatcgag ggtgaccacc gcacgctgct ggagggcagc
     9781 ggcctggagt ccatcatcag catcatccac agctccctgg ctgagccacg cgtgagcgtg
     9841 cgggagggcc tcgagtaccc atacgacgta cctgattatg caacttatcc ttatgatgta
     9901 cctgattatg ctagctatcc ttatgatgtc ccagactacg catagggatc cctgcgatat
     9961 ccagcgcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca
    10021 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga
    10081 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
    10141 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
    10201 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc
    10261 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt
    10321 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc
    10381 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg
    10441 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc
    10501 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc
    10561 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg
    10621 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa
    10681 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac
    10741 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc
    10801 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc
    10861 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc
    10921 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg
    10981 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac
    11041 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc
    11101 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc
    11161 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc
    11221 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc
    11281 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc
    11341 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
    11401 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
    11461 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
    11521 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
    11581 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
    11641 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
    11701 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
    11761 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
    11821 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
    11881 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
    11941 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
    12001 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
    12061 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
    12121 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
    12181 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
    12241 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
    12301 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
    12361 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
    12421 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
    12481 ctccgccttc gccctcagac gagtcggatc tccctttggc cgcctccccg cctggtacct
    12541 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
    12601 ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg
    12661 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
    12721 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
    12781 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
    12841 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
    12901 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    12961 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    13021 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc
    13081 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
    13141 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag
    13201 acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
    13261 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    13321 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    13381 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    13441 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    13501 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    13561 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    13621 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    13681 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    13741 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    13801 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    13861 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    13921 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    13981 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    14041 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
    14101 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    14161 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    14221 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    14281 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    14341 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    14401 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    14461 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    14521 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    14581 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    14641 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    14701 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    14761 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    14821 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    14881 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    14941 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    15001 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    15061 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    15121 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    15181 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    15241 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    15301 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    15361 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
    15421 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
    15481 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
    15541 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
    15601 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
    15661 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
    15721 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
    15781 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
    15841 ccaagctg
//
