LOCUS V035992 7805 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V035992 VERSION V035992 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..2637 /label="FAAP20(NM_001256947)" /note="FAAP20(NM_001256947)" /gene="FAAP20" CDS 2644..2670 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2674..2700 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2704..2730 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 2775..2986 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 2999..3267 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 3294..3893 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 3900..4488 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 4562..4795 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 4867..4988 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 5007..5142 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(5180..5196) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5204..5220 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5228..5258) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5273..5294 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5582..6170) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6341..7201) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7202..7306) /gene="bla" /label="AmpR promoter" primer_bind 7780..7796 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggaggcg gcgcggaggc cgcggctggg gttgagccgc 2341 cggaggccgc gcccggcggg cgggagccca ggtgcggccc ggagcccact gaagtcttca 2401 ctgtcggacc caagaccttt tcctggacac cctttccgcc ggacctgtgg ggcccgggcc 2461 gttcctaccg gctgcttcac ggggcaggag ggcacctgga atcccccgcc aggtccctgc 2521 cccagcgccc ggcacctgat ccctgcaggg cccccagggt ggagcagcag ccgtctgtgg 2581 agggtgccgc ggccctgcgc agctgcccca tgtgccagaa ggagttcgcc cccaggcgga 2641 tcctacccat acgacgtacc tgattatgca acttatcctt atgatgtacc tgattatgct 2701 agctatcctt atgatgtccc agactacgca taggcggccg cgaaggatct gcgatcgctc 2761 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 2821 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 2881 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 2941 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg 3001 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt 3061 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt 3121 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag 3181 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc 3241 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagatgaccg 3301 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc 3361 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca 3421 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca 3481 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg 3541 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc 3601 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt 3661 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg 3721 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct 3781 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg 3841 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgaca 3901 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 3961 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 4021 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 4081 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 4141 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 4201 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 4261 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg 4321 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 4381 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 4441 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta 4501 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 4561 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct 4621 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 4681 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 4741 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 4801 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 4861 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 4921 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 4981 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 5041 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 5101 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 5161 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 5221 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 5281 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 5341 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 5401 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 5461 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 5521 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 5581 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 5641 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 5701 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 5761 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 5821 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 5881 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 5941 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 6001 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 6061 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 6121 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 6181 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 6241 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 6301 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 6361 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 6421 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 6481 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 6541 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 6601 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 6661 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 6721 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 6781 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 6841 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 6901 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 6961 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 7021 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 7081 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 7141 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 7201 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 7261 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 7321 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 7381 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 7441 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 7501 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 7561 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 7621 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 7681 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 7741 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 7801 agctg //