LOCUS       V027808                 9167 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027808
VERSION     V027808
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4092
                     /label="F12(NM_021489)"
                     /note="F12(NM_021489)"
                     /gene="F12"
     promoter        4137..4348
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4361..4629
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             4656..5255
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5262..5850
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5924..6157
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6229..6350
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6369..6504
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(6542..6558)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6566..6582
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6590..6620)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6635..6656
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6944..7532)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7703..8563)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8564..8668)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9142..9158
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgacggct ctgttgttcc tggggtctct gctgatgagt
     2341 ctggatctga cactttcggc tccaccatgg aaagactcca agaaatttaa ggacgcacct
     2401 gatgggccca cagtggttct cactgtggat gggaggctct gccattttcc ctttcagtac
     2461 caccgtcagc tacaccacaa atgcatccac aaaaggcggc caggctcccg cccctggtgt
     2521 gctaccaccc ccaactttga tgaagatcag caatggggat actgcttgga gcccaagaaa
     2581 gtgaaagacc attgcagcaa acacaacccg tgccacaaag gagggacatg tatcaacacc
     2641 cccaatgggc cacactgtct ctgccctgaa cacctcactg ggaaacattg ccagaaagag
     2701 aaatgctttg agcctcagct tctcaagttc ttccacgaga atgagctatg gtttagaacg
     2761 gggccaggag gtgtggccag gtgcgagtgc aaaggttctg aggctcactg caagccggtg
     2821 gccagccagg cctgcagcat caatccgtgc cttaatgggg gcagctgcct cctcgtggag
     2881 gaccacccac tgtgccgttg ccctacaggc tacactggat atttttgcga cttggacctt
     2941 tgggcgacct gctatgaagg cagggggctc agctaccggg gccaggctgg aactacgcaa
     3001 tcgggtgcgc catgtcagcg gtggaccgtg gaggccacct accggaacat gactgagaag
     3061 caagcgctaa gctggggcct gggccaccac gcattttgcc ggaacccaga taatgacaca
     3121 cgtccatggt gcttcgtctg gagtggcgac aggctgagct gggactattg cggcctggag
     3181 cagtgccaga cgccaacgtt tgcacctcta gttgtccctg agagtcagga ggagtccccg
     3241 tcccaggcac catctctgtc ccatgcacca aatgactcga ccgatcatca gacttctctg
     3301 tccaagacca acacgatggg ctgcggacag aggttccgca agggactgtc ctcgttcatg
     3361 cgcgtggtgg gcggactagt ggctctgcct gggtcgcacc cctacatcgc tgcactgtac
     3421 tggggtaaca acttctgcgc gggcagtctc atcgccccct gttgggtgct gaccgcggct
     3481 cactgcctgc agaatcggcc agcgcccgag gaactgacag tggtacttgg tcaagatcgc
     3541 cacaaccaga gctgcgagtg gtgccagact ctggctgtgc gctcctaccg ccttcacgag
     3601 ggcttctcct ccatcaccta ccagcacgac ttggctctgc tgcgcctgca ggaaagcaaa
     3661 accaacagtt gcgcgatcct gtcacctcac gttcagcctg tgtgtctacc cagcggcgcg
     3721 gccccaccct ctgagacagt gctctgcgag gtggccggct ggggtcacca gttcgagggg
     3781 gctgaagaat actccacctt cctgcaggag gcacaggttc cctttatcgc cctggatcgc
     3841 tgctccaact ctaacgtgca cggagacgcc attctccctg ggatgctttg cgctggcttc
     3901 ttggagggag gcaccgatgc ctgccagggt gactccgggg gccctctggt gtgtgaggaa
     3961 ggaactgcag aacatcagct caccctgcgc ggagtcatca gctggggctc cggctgtggt
     4021 gaccgcaaca agcccggagt ctacacagac gtggccaact acctggcttg gatccagaag
     4081 catattgctt cataagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc
     4141 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
     4201 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     4261 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     4321 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
     4381 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
     4441 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
     4501 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
     4561 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
     4621 ttacagatcc aagctgtgac cggcgcctac gctagatgac cgagtacaag cccacggtgc
     4681 gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg
     4741 actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc
     4801 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     4861 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     4921 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     4981 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     5041 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     5101 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     5161 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     5221 gcacctggtg catgacccgc aagcccggtg cctgagtcga caatcaacct ctggattaca
     5281 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     5341 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     5401 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     5461 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5521 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5581 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5641 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     5701 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5761 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5821 gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat gacttacaag
     5881 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     5941 tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     6001 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6061 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6121 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     6181 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     6241 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6301 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     6361 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6421 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     6481 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat
     6541 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6601 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6661 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6721 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6781 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6841 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6901 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6961 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7021 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7081 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7141 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7201 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7261 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7321 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7381 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7441 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7501 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7561 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7621 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7681 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7741 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7801 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7861 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7921 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7981 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8041 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8101 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8161 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8221 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8281 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8341 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8401 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8461 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8521 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8581 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8641 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     8701 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     8761 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     8821 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     8881 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     8941 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     9001 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     9061 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     9121 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
