LOCUS       V029160                12124 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029160
VERSION     V029160
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6150
                     /label="Emsy(NM_001381869)"
                     /note="Emsy(NM_001381869)"
                     /gene="Emsy"
     CDS             6157..6222
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6266..6477
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6490..6758
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6787..6796
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6817..7479
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7549..7602
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7603..8202
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8203..8791
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8865..9098
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9170..9291
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9331..9466
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9499..9515)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9523..9539
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9547..9577)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9592..9613
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9901..10489)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10660..11520)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11521..11625)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12099..12115
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc ctgttgtgtg gccaaccctt
     2341 ttggatctca gcagggatga atgcaaacgg attcttcgaa aattggaact ggaggcatat
     2401 gctggagtta tcagtgcact tcgggcacaa ggggacctca ccaaggaaaa gaaagatctt
     2461 cttggagagc tctcaaaagt ccttagcatc tcaacagagc gccaccgtgc tgaagttcgg
     2521 agagcagtaa acgatgaacg gttaacaaca attgcacata aaatgaattt atccttatat
     2581 ttgggtgaaa gaccaagtta cagtatgtct ggacctaata gttcttcaga gtggtccatc
     2641 gaaggacgta gattggtacc tctgatgccc aggctggttc cccagactgc cttcactgta
     2701 acagcgaatg ctgtggccaa tgcagctgtc cagcacaatg cgtcccttcc tgtgcctgca
     2761 gagacagcaa gcaaagacgg cgttagctgc tcagatgaag atgaaaaacc cagaaaacgc
     2821 aggcgaacaa actcctctag ctcctcccct gttgtcctca aggaagttcc aaaggctgtt
     2881 gttccggtct caaagacgat cactgtgcct gtgagtggaa gtcccaagat gagcaacatt
     2941 atgcagagca ttgccaactc cttaccaccc catatgtcac ctgtgaaaat aacgtttacc
     3001 aaaccatcaa cacagacaac aaacacaaca acacagaagg ttattatagt gaccacatca
     3061 ccaagttcaa cctttgtgcc caacattctc tccaaatctc ataactatgc agcagtcact
     3121 aagcttgtac caacatcagt cattgcctcc acgactcaga agccacctgt tgttataact
     3181 gcgtcccagg cctctctggt caccagtagc agcaatggca acagcagttc tacgtcatca
     3241 cctatttcta gtacagttgc agtaacaacg gtggtatctt ccacaccgtc cgtggtcatg
     3301 tcaacagtag cacaaggtgt atccacatca gcaatcaaag tggcatcgac aagacttcct
     3361 tcccccaaaa gcttagtgag tggccctacc cagattcttg cacagtttcc taaacagcat
     3421 cagcagtctc ctaagcagca gttacagcaa gtacaacaac agacacagca gccagtggcc
     3481 cagccttctt cagtgtctca gcagcagcag cctcagcagt cagcattgcc gcctggtatc
     3541 aaacctacca tccagatcaa acaggagtca ggtgttaaaa tcatcacaca gcaggttcaa
     3601 ccaagtaaaa tcttacccaa accagtgact gcaactctac ccaccagtag caattcccca
     3661 attatggtgg ttagcagtaa tggtgcaatt atgacaacta aactggtaac cactcctacc
     3721 ggtactcagg caacctatac ccggccaaca gtgagcccat ccttaggtcg tgtggctaca
     3781 acccctggag ctgcaaccta tgtgaaaact accagtggca gcatcatcac tgtagtaccc
     3841 aaatcattag ctaccttggg aggcaagata attagcagta atattgtttc tggaacgact
     3901 accaaaatca ctacaatccc aatgacttcc aagcccaatg tgattgttgt acaaaagact
     3961 acaggaaaag gaacgaccat tcaaggcctc cctggcaaaa acgttgtcac aaccttgcta
     4021 aatgctggag gagaaaagac tcttcagaca gtgccagctg gagccaagcc agctataatt
     4081 actgctacaa gacccatcac caaaatgatt gtaacacagc ccaaaggaat aggttccgca
     4141 gtacagccag cagctaaaat cattccaaca aaaattgtgt atggacaaca agggaaaaca
     4201 caggttctaa ttaaacccaa accagtgaca tttcaagcta cagttgtcag tgaacaaaca
     4261 aggcagctgg taacagaaac attacagcaa gcatccaggg tagcagatgc cagtaattca
     4321 tctgctcagg aaggaaaaga ggagccacag ggttacacag acagtagttc ttcttccaca
     4381 gagtcctccc agagttctca agattcccag cctgtagttc atgtaattgc ttcccggcgt
     4441 caggattggt cagaacatga gattgcaatg gagactagcc ccaccataat atatcaggat
     4501 gtatccagtg aatcacaatc agccacttca acaatcaaag ctctgttaga actccaacag
     4561 acaacagtaa aggaaaaatt ggaatctaaa ccaagacaac ccactattga cttgagtcaa
     4621 atggcagtac ctattcagat gacccaggaa aagagacatt ctcctgagag tccatccatt
     4681 gctgtggtag agtcagaact agttgctgaa tacatcacca cggaacgcac tgatgagggg
     4741 acagaggttg cttttcccct tctaggtaac catcgctccc agccccaaca gccttcccag
     4801 ccccagcgga ccctgcttca acatgtggct cagtcacaga ctgcgacgca gacttcggtg
     4861 gtagtaaagt ccatcccagc gtcttcccct ggagcaatca cccacatcat gcagcaggca
     4921 ttaagcagtc acaccgcttt tacgaaacac agtgaggaac ttggaactga ggagggtgag
     4981 gttgaagaga tggacacatt ggatcctcag acaggtctgt tttaccgctc tgccctgaca
     5041 caatcgcagt caaccaaaca gcagaaactt agccagcccc agctggaaca gactcagctg
     5101 caagtgaaaa ctctgcagtg cttccagacc aaacagaagc agaccatcca cctgcaggca
     5161 gaccaactcc agcacaaact cacacagatg ccccagctct ctatcaggca tcagaaactg
     5221 aatcctctcc agcaggaaca agcacagccc aagccagatg cacagcacac acagcatact
     5281 gtggtggcca aagacaggca gcttcctacc ttaatggcac agcccccgca aactgtagta
     5341 caggtgcttg ccgtgaaaac cacacagcag cttcctaaac tgcagcaagc tccgaaccaa
     5401 ccaaaaatct acgtgcaacc ccaaactcct cagagccaga tggcactccc ctcttcagag
     5461 aaacagcctg caagccaggt ggagcagcca attataactc aaggatcctc tgttacaaag
     5521 ataacatttg aggggcgcca gcctcccaca gttacaaaga taactggtgg cagttctgtt
     5581 cctaagctga catcaccagt tacaagcata tctcccattc aggcctctga gaagacagca
     5641 gtgtcagaca ttttgcaaat gtctttgatg gaagctcaga ttgatacaaa tgtagaacat
     5701 atggtagtgg atcccccaaa gaaggctcta gccaccaatg tgctcactgg tgaggcagga
     5761 gctctaccct ccacccatgt ggtggtggca gggatgacga aatgtagaga gtcctgttca
     5821 agtccatctg ctgttggccc tcccctaacg accagaaaaa tcgaagcagc aggagtgcct
     5881 acgacaggcc agttcatgcg tattcagaat gtaggccaaa agaaagctga agagagccca
     5941 acagaaatta tcatccaggc cattccccag tatgctattc cttgtcactc cagctccaat
     6001 gtggtggtgg agcccagtgg gcttctagag ttgaacaact tcaccagtca gcagctagat
     6061 gacgacgaga cagccatgga gcaggacata gacagcagca cggaggacgg gactgagccc
     6121 agcccctctc agagcgctgt agaacggtcc ggatccgact acaaagacca tgacggtgat
     6181 tataaagatc atgacatcga ctacaaggat gacgatgaca agtagcggcc gcgaaggatc
     6241 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     6301 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     6361 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     6421 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     6481 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     6541 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     6601 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     6661 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     6721 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     6781 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc
     6841 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc
     6901 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc
     6961 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc
     7021 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag
     7081 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc
     7141 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc
     7201 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg
     7261 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg
     7321 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc
     7381 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg
     7441 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc
     7501 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga
     7561 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc
     7621 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     7681 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     7741 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     7801 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     7861 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     7921 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     7981 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     8041 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     8101 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     8161 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac
     8221 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     8281 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     8341 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     8401 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     8461 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     8521 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     8581 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     8641 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     8701 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     8761 agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa
     8821 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     8881 ctcccaacga agataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     8941 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     9001 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     9061 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     9121 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     9181 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     9241 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9301 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     9361 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     9421 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga
     9481 gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9541 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9601 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9661 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9721 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9781 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9841 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9901 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9961 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    10021 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    10081 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    10141 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    10201 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    10261 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    10321 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
    10381 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    10441 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10501 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10561 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10621 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10681 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10741 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10801 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10861 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10921 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10981 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    11041 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    11101 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    11161 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    11221 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    11281 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    11341 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    11401 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    11461 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    11521 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    11581 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    11641 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    11701 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    11761 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    11821 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
    11881 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    11941 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
    12001 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    12061 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
    12121 gctg
//
