LOCUS       V032924                12880 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032924
VERSION     V032924
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6906
                     /label="ERICH3(NM_001002912)"
                     /note="ERICH3(NM_001002912)"
                     /gene="ERICH3"
     CDS             6913..6978
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7022..7233
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7246..7514
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7543..7552
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7573..8235
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8305..8358
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8359..8958
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8959..9547
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9621..9854
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9926..10047
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10087..10222
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10255..10271)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10279..10295
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10303..10333)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10348..10369
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10657..11245)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11416..12276)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12277..12381)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12855..12871
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga gccattctca ccccgctggg
     2341 ttacttgctg catataatag ccttatggat aaacacctgg ctgggtattt taacaataca
     2401 aggataaggc gtcatctctt aagatcagga ctgatcacaa gaagtggaag aatactttct
     2461 gaaaaagaat ataaactaaa tatgatgaag cgggatcatc aaaaatatat ccgggaatgc
     2521 ttagcccagg caatttttca taaagttctt gatatggagc gttaccatca gcttgaaata
     2581 aaaaagaaat tggagacctt agctaggaag gagcgaatcc agaggtttaa gggagagcac
     2641 acaagaaggt ctgttgaaaa taacatgcca atcctgtctc cccacccacc agttggccca
     2701 aagagtaatc gtggccatag tgttctggtt gatgaaggac attccagtcc gttagcactg
     2761 acagcccctc gaccatatac tgctccagga aatatgcagc ctccaattcg attacagcct
     2821 cttcccagta atcctgcagt agaaactgtt ccaaaggtaa cttcaaggtc cagatcaaaa
     2881 acctcattgc tggaaaatga agctctgttt cccattgggg gcaagaaggc agtgatgaag
     2941 ttcaggaact ccataggcaa ttcacagaga atgaattcat atcaacttcc aaacattaac
     3001 agttacatga tgcctattcc tcctccactt cccccaactg ggaaaatcac aagagaaaat
     3061 agatctgaaa catggagaag gagaagattt cgtccaacca ctgctccaaa tggcttagaa
     3121 cctcttttga caaaggattc aagaaggatt cataaaacat ccttacatag taatgcagct
     3181 attacaatga tctatttggg gaaaaatgtg cacctatctt ctgataatcc tgacttccgg
     3241 gatgaaatta aagtttatca gcagcactgt ggtggggaaa acctttgtgt ctacaaaggc
     3301 aaactacttg aaaaagagac ctttcagttt atttccaaaa ggcatcatgg tttccccttc
     3361 agtctcacct ttttcctgaa tgggatgcag gtgaacaggt taagctcctg ttgtgaatac
     3421 aagcatcgga aaggttccag gcttggaggc aaacgaggct actttgggtt tgtgtgtgtt
     3481 gagagatcat ctccttgcta caagtgcatt attgcaatgg gccttgacaa aaaaccgtct
     3541 ttgccgaaat ctaggaaaga aaagagcact gagaaaggag aggaactgaa gaaggctgag
     3601 gggaaagtga ggaaagagag agagtatgtg ataccaaaaa gaaatgagat caaggagaac
     3661 aaaacctctg tttcagccaa attttcagct caagaaataa aaacagggct caaagaagtg
     3721 gtaactgctg tggaggaaat gacaagtaaa ggaaaaccag gacaagaagt cttggaagac
     3781 gaccaggaaa atactttaaa atatgagtat gaagaagact ttgaagtaga tgaggagaaa
     3841 caaggtgaaa aatctaatga agaaggacag gctgatgttc aaatgaatgg aataccgcag
     3901 tcacctttgg atgataaaaa agataattta gaccctgaaa aagagagtga aacctcatca
     3961 cagaaggcac cagatgcccg tgacaatgtg aaagatgaga atgatggatg ctctgagagt
     4021 gaactggaag aggataaaca agatatgaaa actgcttcat caacctcatc cagaagtcac
     4081 ccttattcta gtgacagtga ggatgaatct gcagtggggg acagggaagc ccacactgac
     4141 agcagcacag atgaaagtgc cagaaggtca tcttctcagg aactgagtga aaatgataag
     4201 ccaagaaagt ctcaccttcc aattgaggaa tccttagaaa ttgaaattga agaccaagaa
     4261 ataacaaaag cagatgtgga gaccaagccg atgccaatag acgaaagctt tgagaatgtt
     4321 cttaaagaag gaacggagaa aggaacccaa gagattgcag agggtttatc tgagaagtcc
     4381 gggaaacatg tttctgcaga agaaaaggaa aaggataaga gtaagctttg ggaagaaagc
     4441 actgctcagg tgaaggacaa aaaggcaggt ctccctgggt tggaggaagg tggaaaggat
     4501 tcattgccat tagcctatgt cctggctctt ggtgcaccaa caatgaattt catggtggat
     4561 gaaacagcag caatcaactc aaacaaggaa tcccagcaat tggtgcaaaa aacgtataca
     4621 ctggagaaga aagaagcaat ggaggaagat gaagcgcccc agcacagaga tgctgacata
     4681 gtacagggaa aaggggaggc agcactgtgg ggagaagcag gagctgttca tgaggctccc
     4741 ttgagggcgt ggaagccaac agcagagcag ccagaattgg cagaagagtt tacagaaaaa
     4801 agggagatcc ctccaggcat agaaaggggg gcagagggag cagcagaagc agaaggggtc
     4861 agaaggctgg gtgaaggggg gtcagacccc ataggacaag cagcagcaaa agatgctgtg
     4921 ggtctgagta aagatgaggc tcctgaaaag caagccttga tgctcacagt gcttgagaca
     4981 gacaaagcag cttctgaagg ggaacagggt ttagagaagg cagtgcttgc aaatgaagca
     5041 gcagccctga acttggagca tcttcatgaa gtagcagccc tgagagaggc agcgacatcg
     5101 gaggagggag aggctgaggg tggggtggct gtgagtgatg tcggagaaag tgaagaggaa
     5161 gcatccatag acctagagga cacaggaccc atggaggaca cagcatcaaa gagagaggac
     5221 ggttctgaag aggcaattct tgggggagag gaaccagcca aagagagaaa agaggttatg
     5281 agaacagaaa cacgcttgag ccccttcaca ggagaggcag aggcaagccg gatgcaggtt
     5341 tcggagggca gccctgagga aggaagcctt gcaaaggaag ccttcctttg caaggaagat
     5401 gtggaagggg aagagatggt gactgaggca gaagctaata gggaagatga taggaaagaa
     5461 attttaccca aggaattaga tttagcaaga gagcgaagga aagctgagag gccaaaaaca
     5521 tctctgagga aaactgactc tgagagagaa gaggtgacaa gggcaaatgc actcaaggat
     5581 gaagatgctt ttaaagaaga gcaaaaactt aaagcggaag aaggggaaac agagacagaa
     5641 gtaagagctg aggaagagac aaaagctccc ccaaatgaaa tgggatctga tgctgagaac
     5701 gaagcacctg tggaggcttc tgagttgtct gacaatccag ggcttctagg agaagattca
     5761 ctaaaagaga cagtggttcc catatttgaa gcaacgcctg gatttgaaaa gtcgctggaa
     5821 aacataacag ctctgaggaa agaaggagga ggggaaagac tgagtgaagc cagagacaca
     5881 gagcacaaag acagagaaga gctgtccagc agggagaata gggccctgaa ggaagggcac
     5941 cgccaagatg gagagggggc cttagcagct cctgaagctg agccagcagg aaaggtgcag
     6001 gcccctgagg ggctgatccc agccacaggc caggcagagg agctagcagc caaagatcac
     6061 gactcctgcg caggactgga ggggagagct gaagggcaag gaggagtgga tgtcgtgcta
     6121 aggacccagg aagctgttgc tgaggaagat cccataatgg cagaaaagtt cagggaggaa
     6181 gcggtggatg aggacccaga ggaggaagag gacaaagagt gcactctgga gacagaagcg
     6241 atgcaggaca ggaactcgga aggggacggg gacatggaag gagaaggaaa cacacaaaag
     6301 aatgagggca tgggaggagg aagggttgtg gctgtggaag ttctacacgg aggtggtgaa
     6361 acggcagaaa cagccgcaga ggagagggag gtgttggcag gttcggagac agccgaggag
     6421 aaaacaatag caaataaagc ctcctccttt tcagatgttg ctgaggaaga aacctggcac
     6481 caacaggatg agttagtagg aaaaacagca gctgcaggga aggtggtggt agaggaatta
     6541 gcacggagtg gggaggaagt gccagcagca gaggagatga cagtgacata tacaacagag
     6601 gctggggtgg gcactccagg agccctggag cggaagacct cagggctagg acaggagcaa
     6661 gaggaagggt cagagggcca ggaggcagcc actgggagtg gcgatgggag gcaggagaca
     6721 ggagcagctg aaaaattccg attaggatta tcacgggagg gagagaggga attgagtccg
     6781 gagagtctac aggcgatggc aacacttcca gtgaagcctg atttcactga aacccgagag
     6841 aagcaacagc atatggtgca aggagaaagc gagactgcag atgtttcccc caacaacgtg
     6901 caggtcggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     6961 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     7021 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     7081 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     7141 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     7201 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     7261 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     7321 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     7381 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     7441 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     7501 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     7561 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     7621 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     7681 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     7741 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     7801 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     7861 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     7921 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     7981 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     8041 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     8101 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     8161 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     8221 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     8281 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     8341 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     8401 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     8461 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     8521 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     8581 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     8641 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     8701 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     8761 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     8821 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     8881 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     8941 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     9001 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     9061 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     9121 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     9181 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     9241 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     9301 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     9361 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     9421 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     9481 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     9541 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     9601 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct
     9661 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     9721 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     9781 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     9841 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     9901 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     9961 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    10021 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
    10081 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
    10141 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
    10201 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata
    10261 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    10321 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    10381 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    10441 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    10501 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    10561 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    10621 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    10681 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    10741 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    10801 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    10861 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    10921 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    10981 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    11041 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
    11101 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    11161 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    11221 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    11281 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    11341 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    11401 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    11461 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    11521 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    11581 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    11641 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    11701 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    11761 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    11821 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    11881 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    11941 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    12001 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    12061 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    12121 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    12181 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    12241 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    12301 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    12361 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    12421 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    12481 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    12541 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    12601 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    12661 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    12721 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    12781 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    12841 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
