LOCUS       V022483                11809 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022483
VERSION     V022483
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2295..5927
                     /EC_number=""
                     /codon_start=1
                     /gene=""
                     /note=""
                     /product="precursor of human epidermal growth factor
                     receptor"
                     /protein_id=""
                     /transl_table=1
                     /translation="MRPSGTAGAALLALLAALCPASRA,LEEKKVCQGTSNKLTQLGTF
                     EDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLEN
                     LQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESI
                     QWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSG
                     RCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVN
                     PEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNG
                     IGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTV
                     KEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEIS
                     DGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCW
                     GPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRG
                     PDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLE
                     GCPTNGPKIPS,IATGMVGALLLLLVVALGIGLFM,RRRHIVRKRTLRRLLQERELVEP
                     LTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREA
                     TSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNI
                     GSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKE
                     YHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSIL
                     EKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDER
                     MHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNS
                     TVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGS
                     VQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSH
                     QISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA*"
                     /label="EGFR"
     promoter        5968..6179
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6192..6460
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6489..6498
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6519..7181
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7251..7304
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7305..7904
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7905..8492
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8566..8799
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8871..8992
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9011..9146
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9184..9200)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9208..9224
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9232..9262)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9277..9298
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9586..10174)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10345..11205)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11206..11310)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11784..11800
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc caccatgcga ccctccggga cggccggggc agcgctcctg gcgctgctgg
     2341 ctgcgctctg cccggcgagt cgggctctgg aggaaaagaa agtttgccaa ggcacgagta
     2401 acaagctcac gcagttgggc acttttgaag atcattttct cagcctccag aggatgttca
     2461 ataactgtga ggtggtcctt gggaatttgg aaattaccta tgtgcagagg aattatgatc
     2521 tttccttctt aaagaccatc caggaggtgg ctggttatgt cctcattgcc ctcaacacag
     2581 tggagcgaat tcctttggaa aacctgcaga tcatcagagg aaatatgtac tacgaaaatt
     2641 cctatgcctt agcagtctta tctaactatg atgcaaataa aaccggactg aaggagctgc
     2701 ccatgagaaa tttacaggaa atcctgcatg gcgccgtgcg gttcagcaac aaccctgccc
     2761 tgtgcaacgt ggagagcatc cagtggcggg acatagtcag cagtgacttt ctcagcaaca
     2821 tgtcgatgga cttccagaac cacctgggca gctgccaaaa gtgtgatcca agctgtccca
     2881 atgggagctg ctggggtgca ggagaggaga actgccagaa actgaccaaa atcatctgtg
     2941 cccagcagtg ctccgggcgc tgccgtggca agtcccccag tgactgctgc cacaaccagt
     3001 gtgctgcagg ctgcacaggc ccccgggaga gcgactgcct ggtctgccgc aaattccgag
     3061 acgaagccac gtgcaaggac acctgccccc cactcatgct ctacaacccc accacgtacc
     3121 agatggatgt gaaccccgag ggcaaataca gctttggtgc cacctgcgtg aagaagtgtc
     3181 cccgtaatta tgtggtgaca gatcacggct cgtgcgtccg agcctgtggg gccgacagct
     3241 atgagatgga ggaagacggc gtccgcaagt gtaagaagtg cgaagggcct tgccgcaaag
     3301 tgtgtaacgg aataggtatt ggtgaattta aagactcact ctccataaat gctacgaata
     3361 ttaaacactt caaaaactgc acctccatca gtggcgatct ccacatcctg ccggtggcat
     3421 ttaggggtga ctccttcaca catactcctc ctctggatcc acaggaactg gatattctga
     3481 aaaccgtaaa ggaaatcaca gggtttttgc tgattcaggc ttggcctgaa aacaggacgg
     3541 acctccatgc ctttgagaac ctagaaatca tacgcggcag gaccaagcaa catggtcagt
     3601 tttctcttgc agtcgtcagc ctgaacataa catccttggg attacgctcc ctcaaggaga
     3661 taagtgatgg agatgtgata atttcaggaa acaaaaattt gtgctatgca aatacaataa
     3721 actggaaaaa actgtttggg acctccggtc agaaaaccaa aattataagc aacagaggtg
     3781 aaaacagctg caaggccaca ggccaggtct gccatgcctt gtgctccccc gagggctgct
     3841 ggggcccgga gcccagggac tgcgtctctt gccggaatgt cagccgaggc agggaatgcg
     3901 tggacaagtg caaccttctg gagggtgagc caagggagtt tgtggagaac tctgagtgca
     3961 tacagtgcca cccagagtgc ctgcctcagg ccatgaacat cacctgcaca ggacggggac
     4021 cagacaactg tatccagtgt gcccactaca ttgacggccc ccactgcgtc aagacctgcc
     4081 cggcaggagt catgggagaa aacaacaccc tggtctggaa gtacgcagac gccggccatg
     4141 tgtgccacct gtgccatcca aactgcacct acggatgcac tgggccaggt cttgaaggct
     4201 gtccaacgaa tgggcctaag atcccgtcca tcgccactgg gatggtgggg gccctcctct
     4261 tgctgctggt ggtggccctg gggatcggcc tcttcatgcg aaggcgccac atcgttcgga
     4321 agcgcacgct gcggaggctg ctgcaggaga gggagcttgt ggagcctctt acacccagtg
     4381 gagaagctcc caaccaagct ctcttgagga tcttgaagga aactgaattc aaaaagatca
     4441 aagtgctggg ctccggtgcg ttcggcacgg tgtataaggg actctggatc ccagaaggtg
     4501 agaaagttaa aattcccgtc gctatcaagg aattaagaga agcaacatct ccgaaagcca
     4561 acaaggaaat cctcgatgaa gcctacgtga tggccagcgt ggacaacccc cacgtgtgcc
     4621 gcctgctggg catctgcctc acctccaccg tgcagctcat cacgcagctc atgcccttcg
     4681 gctgcctcct ggactatgtc cgggaacaca aagacaatat tggctcccag tacctgctca
     4741 actggtgtgt gcagatcgca aagggcatga actacttgga ggaccgtcgc ttggtgcacc
     4801 gcgacctggc agccaggaac gtactggtga aaacaccgca gcatgtcaag atcacagatt
     4861 ttgggctggc caaactgctg ggtgcggaag agaaagaata ccatgcagaa ggaggcaaag
     4921 tgcctatcaa gtggatggca ttggaatcaa ttttacacag aatctatacc caccagagtg
     4981 atgtctggag ctacggggtg actgtttggg agttgatgac ctttggatcc aagccatatg
     5041 acggaatccc tgccagcgag atctcctcca tcctggagaa aggagaacgc ctccctcagc
     5101 cacccatatg taccatcgat gtctacatga tcatggtcaa gtgctggatg atagacgcag
     5161 atagtcgccc aaagttccgt gagttgatca tcgaattctc caaaatggcc cgagaccccc
     5221 agcgctacct tgtcattcag ggggatgaaa gaatgcattt gccaagtcct acagactcca
     5281 acttctaccg tgccctgatg gatgaagaag acatggacga cgtggtggat gccgacgagt
     5341 acctcatccc acagcagggc ttcttcagca gcccctccac gtcacggact cccctcctga
     5401 gctctctgag tgcaaccagc aacaattcca ccgtggcttg cattgataga aatgggctgc
     5461 aaagctgtcc catcaaggaa gacagcttct tgcagcgata cagctcagac cccacaggcg
     5521 ccttgactga ggacagcata gacgacacct tcctcccagt gcctgaatac ataaaccagt
     5581 ccgttcccaa aaggcccgct ggctctgtgc agaatcctgt ctatcacaat cagcctctga
     5641 accccgcgcc cagcagagac ccacactacc aggaccccca cagcactgca gtgggcaacc
     5701 ccgagtatct caacactgtc cagcccacct gtgtcaacag cacattcgac agccctgccc
     5761 actgggccca gaaaggcagc caccaaatta gcctggacaa ccctgactac cagcaggact
     5821 tctttcccaa ggaagccaag ccaaatggca tctttaaggg ctccacagct gaaaatgcag
     5881 aatacctaag ggtcgcgcca caaagcagtg aatttattgg agcatagcgg ccgcgaagga
     5941 tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     6001 agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact
     6061 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     6121 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc
     6181 tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc
     6241 atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt
     6301 ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt
     6361 ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct
     6421 acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta
     6481 cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag atcgagtgcc
     6541 gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga gagggcaccc
     6601 ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg accttcagcc
     6661 cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc taccccagcg
     6721 gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac acccgcatcg
     6781 agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac gaggccggcc
     6841 gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc gtgatcttca
     6901 ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg ggcgataacg
     6961 tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac tacagcttcg
     7021 tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg cagaacgggg
     7081 gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag ctgggcatcg
     7141 tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc gctcagtcgt
     7201 ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc gagggcagag
     7261 gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc
     7321 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     7381 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     7441 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     7501 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     7561 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     7621 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     7681 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     7741 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     7801 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     7861 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt
     7921 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     7981 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     8041 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     8101 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     8161 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     8221 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     8281 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     8341 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     8401 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga
     8461 cgagtcggat ctccctttgg ccgcctcccc gcctggtacc tttaagacca atgacttaca
     8521 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     8581 actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     8641 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8701 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8761 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     8821 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     8881 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8941 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     9001 ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     9061 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     9121 gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc
     9181 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     9241 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     9301 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     9361 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     9421 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     9481 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     9541 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     9601 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9661 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     9721 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     9781 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     9841 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     9901 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9961 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    10021 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    10081 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    10141 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    10201 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    10261 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    10321 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    10381 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    10441 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    10501 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    10561 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    10621 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    10681 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    10741 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    10801 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    10861 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    10921 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10981 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    11041 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    11101 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    11161 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    11221 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    11281 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    11341 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
    11401 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
    11461 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    11521 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
    11581 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
    11641 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
    11701 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
    11761 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
