LOCUS       V030280                10357 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030280
VERSION     V030280
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5010
                     /label="ZZZ3(NM_015534)"
                     /note="ZZZ3(NM_015534)"
                     /gene="ZZZ3"
     CDS             5017..5082
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5126..5337
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5350..5618
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5651..6445
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6452..7040
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7114..7347
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7419..7540
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7559..7694
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7732..7748)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7756..7772
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7780..7810)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7825..7846
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8134..8722)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8893..9753)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9754..9858)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10332..10348
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggctgct tcccgatcta ctcgtgttac aagatcaaca
     2341 gtggggttaa acggcttgga tgaatctttt tgtggtagaa ctttaaggaa tcgtagcatt
     2401 gcgcatcctg aagaaatctc ttctaattct caagtacgat caagatcacc aaagaagaga
     2461 ccagagcctg tgccaattca gaaaggaaat aataatggga gaaccactga tttaaaacag
     2521 cagagtaccc gagaatcatg ggtaagccct aggaaaagag gactttcttc ttcagaaaag
     2581 gataacatag aaaggcaggc tatagaaaat tgtgagagaa ggcaaacaga acctgtttca
     2641 ccagttttaa aaagaattaa gcgttgtctt agatctgaag caccaaacag ttcagaagaa
     2701 gattctccta taaaatcaga caaggagtca gtagaacaga ggagtacagt agtggacaat
     2761 gatgcagatt ttcaagggac taaacgagct tgtcgatgtc ttatactgga tgattgtgag
     2821 aaaagggaaa ttaaaaaggt gaatgtcagt gaggaagggc cacttaattc tgcagtagtt
     2881 gaagaaatca caggctattt ggctgtcaat ggtgttgatg acagtgattc agctgttata
     2941 aactgtgatg actgtcagcc tgatgggaac actaaacaaa atagcattgg ttcctatgtg
     3001 ttacaggaaa aatcagtagc tgaaaatggg gatacggata cccaaacttc aatgttcctt
     3061 gatagtagga aggaggacag ttatatagac cataaggtgc cttgcacaga ttcacaagtg
     3121 caggtcaagt tggaggacca caaaatagta actgcctgct tgcctgtgga acatgttaat
     3181 cagctgacta ctgagccagc tacagggccc ttttctgaaa ctcagtcatc tttaagggat
     3241 tctgaggagg aagtagatgt ggtgggagat agcagtgcct caaaagagca gtgtaaagaa
     3301 aacaccaata acgaactgga cacaagtctt gagagtatgc cagcctccgg agaacctgaa
     3361 ccatctcctg ttctagactg tgtttcagct caaatgatgt ctttatcaga acctcaagaa
     3421 catcgttata ctctgagaac ctcaccacga agggcagccc ctaccagagg tagtcccact
     3481 aaaaacagtt ctccttacag agaaaatgga caatttgagg agaataatct tagtcctaat
     3541 gaaacaaatg caactgttag tgataatgta agtcaatctc ctacaaatcc tggtgaaatt
     3601 tctcaaaatg aaaaagggat atgttgtgac tctcaaaata atggaagtga aggagtaagt
     3661 aaaccaccct cagaggcaag actcaatatt ggacatttgc catctgccaa agagagtgcc
     3721 agtcagcaca ttacagaaga ggaagatgat gatcctgatg tttattactt tgaatcagat
     3781 catgtggcac tgaaacacaa caaagattat cagagactat tacagacgat tgctgtactc
     3841 gaggctcagc gttctcaagc agtccaagac cttgaaagtt taggcaggca ccagagagaa
     3901 gcactgaaaa atcccattgg atttgtggaa aaactccaga agaaggctga tattgggctt
     3961 ccatatccac agagagttgt tcaattgcct gagatcgtat gggaccaata tacccatagc
     4021 cttgggaatt ttgaaagaga atttaaaaat cgtaaaagac atactagaag agttaagcta
     4081 gtttttgata aagtaggttt acctgctaga ccaaaaagtc ctttagatcc taagaaggat
     4141 ggagagtccc tttcatattc tatgttgcct ttgagtgatg gtccagaagg ctcaagcagt
     4201 cgtcctcaga tgataagagg acgcttgtgt gatgatacca aacctgaaac atttaaccag
     4261 ttgtggactg ttgaagaaca gaaaaagctg gaacagctac tcatcaaata ccctcctgaa
     4321 gaagtagaat ctcgacgctg gcagaagata gcagatgaat tgggcaacag gacagcaaaa
     4381 caggttgcca gccgagtaca gaagtatttc ataaagctaa ctaaagctgg cattccagta
     4441 ccaggcagaa caccaaactt atatatatac tccaaaaagt cttcaacaag cagacgacag
     4501 caccctctta ataagcatct ctttaagcct tccactttca tgacttcaca tgaaccgcca
     4561 gtgtatatgg atgaagatga tgaccgatct tgttttcata gccacatgaa cactgctgtt
     4621 gaagatgcat cagatgacga aagtattcct atcatgtata ggaatttacc tgaatataaa
     4681 gaactattac agtttaaaaa gttaaagaag cagaaacttc agcaaatgca agctgaaagt
     4741 ggatttgtgc aacatgtggg ctttaagtgt gataactgtg gcatagaacc catccagggt
     4801 gttcggtggc attgccagga ttgtcctcca gaaatgtctt tggatttctg tgattcttgt
     4861 tcagactgtc tacatgaaac agatattcac aaggaagatc accaattaga acctatttat
     4921 aggtcagaga cattcttaga cagagactac tgtgtgtctc agggcaccag ttacaattac
     4981 cttgacccaa actactttcc agcaaacaga ctcgaggact acaaagacca tgacggtgat
     5041 tataaagatc atgacatcga ctacaaggat gacgatgaca agtagcggcc gcgaaggatc
     5101 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     5161 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     5221 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     5281 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     5341 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     5401 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     5461 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     5521 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     5581 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     5641 ctaggccacc atggctgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     5701 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     5761 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5821 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5881 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5941 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     6001 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     6061 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     6121 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     6181 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     6241 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     6301 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     6361 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     6421 tcgccttctt gacgagttct tctgagtcga caatcaacct ctggattaca aaatttgtga
     6481 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6541 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6601 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6661 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6721 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6781 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6841 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6901 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6961 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     7021 cctttgggcc gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag
     7081 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     7141 aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     7201 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7261 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7321 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     7381 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     7441 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7501 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     7561 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7621 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     7681 cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat ggtcatagct
     7741 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     7801 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     7861 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     7921 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     7981 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8041 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8101 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8161 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8221 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8281 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8341 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8401 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8461 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8521 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     8581 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8641 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8701 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8761 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     8821 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8881 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8941 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9001 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9061 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9121 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9181 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9241 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9301 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9361 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9421 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9481 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9541 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9601 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9661 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9721 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     9781 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9841 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     9901 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
     9961 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
    10021 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
    10081 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    10141 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
    10201 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
    10261 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
    10321 gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
