LOCUS       V026396                11482 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026396
VERSION     V026396
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..6135
                     /label="NPC1(NM_000271)"
                     /note="NPC1(NM_000271)"
                     /gene="NPC1"
     CDS             6142..6207
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6251..6462
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6475..6743
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6776..7570
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    7577..8165
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8239..8472
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8544..8665
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8684..8819
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8857..8873)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8881..8897
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8905..8935)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8950..8971
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9259..9847)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10018..10878)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10879..10983)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11457..11473
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgaccgct cgcggcctgg cccttggcct cctcctgctg
     2341 ctactgtgtc cagcgcaggt gttttcacag tcctgtgttt ggtatggaga gtgtggaatt
     2401 gcatatgggg acaagaggta caattgcgaa tattctggcc caccaaaacc attgccaaag
     2461 gatggatatg acttagtgca ggaactctgt ccaggattct tctttggcaa tgtcagtctc
     2521 tgttgtgatg ttcggcagct tcagacacta aaagacaacc tgcagctgcc tctacagttt
     2581 ctgtccagat gtccatcctg tttttataac ctactgaacc tgttttgtga gctgacatgt
     2641 agccctcgac agagtcagtt tttgaatgtt acagctactg aagattatgt tgatcctgtt
     2701 acaaaccaga cgaaaacaaa tgtgaaagag ttacaatact acgtcggaca gagttttgcc
     2761 aatgcaatgt acaatgcctg ccgggatgtg gaggccccct caagtaatga caaggccctg
     2821 ggactcctgt gtgggaagga cgctgacgcc tgtaatgcca ccaactggat tgaatacatg
     2881 ttcaataagg acaatggaca ggcacctttt accatcactc ctgtgttttc agattttcca
     2941 gtccatggga tggagcccat gaacaatgcc accaaaggct gtgacgagtc tgtggatgag
     3001 gtcacagcac catgtagctg ccaagactgc tctattgtct gtggccccaa gccccagccc
     3061 ccacctcctc ctgctccctg gacgatcctt ggcttggacg ccatgtatgt catcatgtgg
     3121 atcacctaca tggcgttttt gcttgtgttt tttggagcat tttttgcagt gtggtgctac
     3181 agaaaacggt attttgtctc cgagtacact cccatcgata gcaatatagc tttttctgtt
     3241 aatgcaagtg acaaaggaga ggcgtcctgc tgtgaccctg tcagcgcagc atttgagggc
     3301 tgcttgaggc ggctgttcac acgctggggg tctttctgcg tccgaaaccc tggctgtgtc
     3361 attttcttct cgctggtctt cattactgcg tgttcgtcag gcctggtgtt tgtccgggtc
     3421 acaaccaatc cagttgacct ctggtcagcc cccagcagcc aggctcgcct ggaaaaagag
     3481 tactttgacc agcactttgg gcctttcttc cggacggagc agctcatcat ccgggcccct
     3541 ctcactgaca aacacattta ccagccatac ccttcgggag ctgatgtacc ctttggacct
     3601 ccgcttgaca tacagatact gcaccaggtt cttgacttac aaatagccat cgaaaacatt
     3661 actgcctctt atgacaatga gactgtgaca cttcaagaca tctgcttggc ccctctttca
     3721 ccgtataaca cgaactgcac cattttgagt gtgttaaatt acttccagaa cagccattcc
     3781 gtgctggacc acaagaaagg ggacgacttc tttgtgtatg ccgattacca cacgcacttt
     3841 ctgtactgcg tacgggctcc tgcctctctg aatgatacaa gtttgctcca tgacccttgt
     3901 ctgggtacgt ttggtggacc agtgttcccg tggcttgtgt tgggaggcta tgatgatcaa
     3961 aactacaata acgccactgc ccttgtgatt accttccctg tcaataatta ctataatgat
     4021 acagagaagc tccagagggc ccaggcctgg gaaaaagagt ttattaattt tgtgaaaaac
     4081 tacaagaatc ccaatctgac catttccttc actgctgaac gaagtattga agatgaacta
     4141 aatcgtgaaa gtgacagtga tgtcttcacc gttgtaatta gctatgccat catgtttcta
     4201 tatatttccc tagccttggg gcacatgaaa agctgtcgca ggcttctggt ggattcgaag
     4261 gtctcactag gcatcgcggg catcttgatc gtgctgagct cggtggcttg ctccttgggt
     4321 gtcttcagct acattgggtt gcccttgacc ctcattgtga ttgaagtcat cccgttcctg
     4381 gtgctggctg ttggagtgga caacatcttc attctggtgc aggcctacca gagagatgaa
     4441 cgtcttcaag gggaaaccct ggatcagcag ctgggcaggg tcctaggaga agtggctccc
     4501 agtatgttcc tgtcatcctt ttctgagact gtagcatttt tcttaggagc attgtccgtg
     4561 atgccagccg tgcacacctt ctctctcttt gcgggattgg cagtcttcat tgactttctt
     4621 ctgcagatta cctgtttcgt gagtctcttg gggttagaca ttaaacgtca agagaaaaat
     4681 cggctagaca tcttttgctg tgtcagaggt gctgaagatg gaacaagcgt ccaggcctca
     4741 gagagctgtt tgtttcgctt cttcaaaaac tcctattctc cacttctgct aaaggactgg
     4801 atgagaccaa ttgtgatagc aatatttgtg ggtgttctgt cattcagcat cgcagtcctg
     4861 aacaaagtag atattggatt ggatcagtct ctttcgatgc cagatgactc ctacatggtg
     4921 gattatttca aatccatcag tcagtacctg catgcgggtc cgcctgtgta ctttgtcctg
     4981 gaggaagggc acgactacac ttcttccaag gggcagaaca tggtgtgcgg cggcatgggc
     5041 tgcaacaatg attccctggt gcagcagata tttaacgcgg cgcagctgga caactatacc
     5101 cgaataggct tcgccccctc gtcctggatc gacgattatt tcgactgggt gaagccacag
     5161 tcgtcttgct gtcgagtgga caatatcact gaccagttct gcaatgcttc agtggttgac
     5221 cctgcctgcg ttcgctgcag gcctctgact ccggaaggca aacagaggcc tcagggggga
     5281 gacttcatga gattcctgcc catgttcctt tcggataacc ctaaccccaa gtgtggcaaa
     5341 gggggacatg ctgcctatag ttctgcagtt aacatcctcc ttggccatgg caccagggtc
     5401 ggagccacgt acttcatgac ctaccacacc gtgctgcaga cctctgctga ctttattgac
     5461 gctctgaaga aagcccgact tatagccagt aatgtcaccg aaaccatggg cattaacggc
     5521 agtgcctacc gagtatttcc ttacagtgtg ttttatgtct tctacgaaca gtacctgacc
     5581 atcattgacg acactatctt caacctcggt gtgtccctgg gcgcgatatt tctggtgacc
     5641 atggtcctcc tgggctgtga gctctggtct gcagtcatca tgtgtgccac catcgccatg
     5701 gtcttggtca acatgtttgg agttatgtgg ctctggggca tcagtctgaa cgctgtatcc
     5761 ttggtcaacc tggtgatgag ctgtggcatc tccgtggagt tctgcagcca cataaccaga
     5821 gcgttcacgg tgagcatgaa aggcagccgc gtggagcgcg cggaagaggc acttgcccac
     5881 atgggcagct ccgtgttcag tggaatcaca cttacaaaat ttggagggat tgtggtgttg
     5941 gcttttgcca aatctcaaat tttccagata ttctacttca ggatgtattt ggccatggtc
     6001 ttactgggag ccactcacgg attaatattt ctccctgtct tactcagtta catagggcca
     6061 tcagtaaata aagccaaaag ttgtgccact gaagagcgat acaaaggaac agagcgcgaa
     6121 cggcttctaa atttcctcga ggactacaaa gaccatgacg gtgattataa agatcatgac
     6181 atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga tcgctccggt
     6241 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     6301 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     6361 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     6421 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     6481 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     6541 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     6601 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     6661 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     6721 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctagg ccaccatggc
     6781 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     6841 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     6901 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     6961 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     7021 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     7081 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     7141 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     7201 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     7261 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     7321 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     7381 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     7441 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     7501 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     7561 gttcttctga gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     7621 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7681 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7741 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     7801 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7861 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7921 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     7981 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     8041 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     8101 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     8161 cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     8221 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa gatctgcttt
     8281 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8341 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     8401 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8461 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     8521 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     8581 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8641 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     8701 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8761 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8821 agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc ctgtgtgaaa
     8881 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8941 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     9001 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9061 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9121 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9181 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9241 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9301 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9361 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9421 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9481 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9541 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9601 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9661 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     9721 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9781 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9841 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9901 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9961 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    10021 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10081 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10141 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    10201 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10261 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10321 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10381 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10441 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10501 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10561 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10621 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10681 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10741 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10801 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10861 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10921 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10981 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
    11041 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
    11101 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
    11161 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
    11221 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
    11281 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
    11341 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
    11401 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
    11461 aacgacggcc agtgccaagc tg
//
