LOCUS       V030224                11149 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030224
VERSION     V030224
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5802
                     /label="ITGA10(NM_003637)"
                     /note="ITGA10(NM_003637)"
                     /gene="ITGA10"
     CDS             5809..5874
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5918..6129
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6142..6410
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6443..7237
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    7244..7832
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7906..8139
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8211..8332
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8351..8486
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8524..8540)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8548..8564
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8572..8602)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8617..8638
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8926..9514)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9685..10545)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10546..10650)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11124..11140
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggaactc cccttcgtca ctcacctgtt cttgcccctg
     2341 gtgttcctga caggtctctg ctcccccttt aacctggatg aacatcaccc acgcctattc
     2401 ccagggccac cagaagctga atttggatac agtgtcttac aacatgttgg gggtggacag
     2461 cgatggatgc tggtgggcgc cccctgggat gggccttcag gcgaccggag gggggacgtt
     2521 tatcgctgcc ctgtaggggg ggcccacaat gccccatgtg ccaagggcca cttaggtgac
     2581 taccaactgg gaaattcatc tcatcctgct gtgaatatgc acctggggat gtctctgtta
     2641 gagacagatg gtgatggggg attcatggcc tgtgcccctc tctggtctcg tgcttgtggc
     2701 agctctgtct tcagttctgg gatatgtgcc cgtgtggatg cttcattcca gcctcaggga
     2761 agcctggcac ccactgccca acgctgccca acatacatgg atgttgtcat tgtcttggat
     2821 ggctccaaca gcatctaccc ctggtctgaa gttcagacct tcctacgaag actggtaggg
     2881 aaactgttta ttgacccaga acagatacag gtgggactgg tacagtatgg ggagagccct
     2941 gtacatgagt ggtccctggg agatttccga acgaaggaag aagtggtgag agcagcaaag
     3001 aacctcagtc ggcgggaggg acgagaaaca aagactgccc aagcaataat ggtggcctgc
     3061 acagaagggt tcagtcagtc ccatgggggc cgacccgagg ctgccaggct actggtggtt
     3121 gtcactgatg gagagtccca tgatggagag gagcttcctg cagcactaaa ggcctgtgag
     3181 gctggaagag tgacacgcta tgggattgca gtccttggtc actacctccg gcggcagcga
     3241 gatcccagct ctttcctgag agaaattaga actattgcca gtgatccaga tgagcgattc
     3301 ttcttcaatg tcacagatga ggctgctctg actgacattg tggatgcact aggagatcgg
     3361 atttttggcc ttgaagggtc ccatgcagaa aacgaaagct cctttgggct ggaaatgtct
     3421 cagattggtt tctccactca tcggctaaag gatgggattc tttttgggat ggtgggggcc
     3481 tatgactggg gaggctctgt gctatggctt gaaggaggcc accgcctttt ccccccacga
     3541 atggcactgg aagacgagtt cccccctgca ttgcagaacc atgcagccta cctgggttac
     3601 tctgtttctt ccatgctttt gcggggtgga cgccgcctgt ttctctctgg ggctcctcga
     3661 tttagacatc gaggaaaagt catcgccttc cagcttaaga aagatggggc tgtgagggtt
     3721 gcccagagcc tccaggggga gcagattggt tcatactttg gcagtgagct ctgcccattg
     3781 gatacagata gggatggaac aactgatgtc ttacttgtgg ctgcccccat gttcctggga
     3841 ccccagaaca aggaaacagg acgtgtttat gtgtatctgg taggccagca gtccttgctg
     3901 accctccaag gaacacttca gccagaaccc ccccaggatg ctcggtttgg ctttgccatg
     3961 ggagctcttc ctgatctgaa ccaagatggt tttgctgatg tggctgtggg ggcgcctctg
     4021 gaagatgggc accagggagc actgtacctg taccatggaa cccagagtgg agtcaggccc
     4081 catcctgccc agaggattgc tgctgcctcc atgccacatg ccctcagcta ctttggccga
     4141 agtgtggatg gtcggctaga tctggatgga gatgatctgg tcgatgtggc tgtgggtgcc
     4201 cagggggcag ccatcctgct cagctcccgg cccattgtcc atctgacccc atcactggag
     4261 gtgaccccac aggccatcag tgtggttcag agggactgta ggcggcgagg ccaagaggca
     4321 gtctgtctga ctgcagccct ttgcttccaa gtgacctccc gtactcctgg tcgctgggat
     4381 caccaattct acatgaggtt caccgcatca ctggatgaat ggactgctgg ggcacgtgca
     4441 gcatttgatg gctctggcca gaggttgtcc cctcggaggc tccggctcag tgtggggaat
     4501 gtcacttgtg agcagctaca cttccatgtg ctggatacat cagattacct ccggccagtg
     4561 gccttgactg tgacctttgc cttggacaat actacaaagc cagggcctgt gctgaatgag
     4621 ggctcaccca cctctataca aaagctggtc cccttctcaa aggattgtgg ccctgacaat
     4681 gaatgtgtca cagacctggt gcttcaagtg aatatggaca tcagaggctc caggaaggcc
     4741 ccatttgtgg ttcgaggtgg ccggcggaaa gtgctggtat ctacaactct ggagaacaga
     4801 aaggaaaatg cttacaatac gagcctgagt ctcatcttct ctagaaacct ccacctggcc
     4861 agtctcactc ctcagagaga gagcccaata aaggtggaat gtgccgcccc ttctgctcat
     4921 gcccggctct gcagtgtggg gcatcctgtc ttccagactg gagccaaggt gacctttctg
     4981 ctagagtttg agtttagctg ctcctctctc ctgagccagg tcttcgtgaa gctgactgcc
     5041 agcagtgaca gcctggagag aaatgggacc cttcaagata acacagccca gacctcagcc
     5101 tacatccaat atgagcccca cctcctgttc tctagtgagt ctaccctgca ccgctatgag
     5161 gttcacccat atgggaccct cccagtgggt cctggcccag aattcaaaac cactctcagg
     5221 gttcagaacc taggctgcta tgtggtcagt ggcctcatca tctcagccct ccttccagct
     5281 gtggcccatg ggggcaatta cttcctatca ctgtctcaag tcatcactaa caatgcaagc
     5341 tgcatagtgc agaacctgac tgaaccccca ggcccacctg tgcatccaga ggagcttcaa
     5401 cacacaaaca gactgaatgg gagcaatact cagtgtcagg tggtgaggtg ccaccttggg
     5461 cagctggcaa aggggactga ggtctctgtt ggactattga ggctggttca caatgaattt
     5521 ttccgaagag ccaagttcaa gtccctgacg gtggtcagca cctttgagct gggaaccgaa
     5581 gagggcagtg tcctacagct gactgaagcc tcccgttgga gtgagagcct cttggaggtg
     5641 gttcagaccc ggcctatcct catctccctg tggatcctca taggcagtgt cctgggaggg
     5701 ttgctcctgc ttgctctcct tgtcttctgc ctgtggaagc ttggcttctt tgcccataag
     5761 aaaatccctg aggaagaaaa aagagaagag aagttggagc aactcgagga ctacaaagac
     5821 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagcgg
     5881 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     5941 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     6001 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     6061 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     6121 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     6181 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     6241 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     6301 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     6361 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     6421 ccggcgccta cgctaggcca ccatggctga acaagatgga ttgcacgcag gttctccggc
     6481 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     6541 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     6601 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     6661 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     6721 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     6781 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     6841 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     6901 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     6961 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     7021 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     7081 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     7141 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     7201 catcgccttc tatcgccttc ttgacgagtt cttctgagtc gacaatcaac ctctggatta
     7261 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     7321 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     7381 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     7441 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     7501 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     7561 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     7621 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     7681 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     7741 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     7801 gagtcggatc tccctttggg ccgcctcccc gcctggtacc tttaagacca atgacttaca
     7861 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     7921 actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     7981 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8041 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8101 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     8161 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     8221 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8281 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     8341 ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     8401 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     8461 gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc
     8521 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     8581 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8641 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8701 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8761 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8821 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8881 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8941 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9001 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     9061 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     9121 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     9181 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     9241 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9301 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9361 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9421 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9481 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9541 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9601 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9661 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9721 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9781 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9841 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9901 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9961 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    10021 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    10081 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    10141 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    10201 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    10261 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10321 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10381 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10441 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10501 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10561 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10621 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10681 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
    10741 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
    10801 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    10861 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
    10921 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
    10981 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
    11041 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
    11101 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
