LOCUS       V042514                10813 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042514
VERSION     V042514
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4779
                     /label="HIF1A(NM_001530)"
                     /note="HIF1A(NM_001530)"
                     /gene="HIF1A"
     CDS             4816..5535
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced GFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="mEGFP"
     promoter        5582..5793
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5806..6074
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6107..6901
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6908..7496
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7570..7803
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7875..7996
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8015..8150
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8188..8204)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8212..8228
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8236..8266)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8281..8302
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8590..9178)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9349..10209)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10210..10314)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10788..10804
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggagggc gccggcggcg cgaacgacaa gaaaaagata
     2341 agttctgaac gtcgaaaaga aaagtctcga gatgcagcca gatctcggcg aagtaaagaa
     2401 tctgaagttt tttatgagct tgctcatcag ttgccacttc cacataatgt gagttcgcat
     2461 cttgataagg cctctgtgat gaggcttacc atcagctatt tgcgtgtgag gaaacttctg
     2521 gatgctggtg atttggatat tgaagatgac atgaaagcac agatgaattg cttttatttg
     2581 aaagccttgg atggttttgt tatggttctc acagatgatg gtgacatgat ttacatttct
     2641 gataatgtga acaaatacat gggattaact cagtttgaac taactggaca cagtgtgttt
     2701 gattttactc atccatgtga ccatgaggaa atgagagaaa tgcttacaca cagaaatggc
     2761 cttgtgaaaa agggtaaaga acaaaacaca cagcgaagct tttttctcag aatgaagtgt
     2821 accctaacta gccgaggaag aactatgaac ataaagtctg caacatggaa ggtattgcac
     2881 tgcacaggcc acattcacgt atatgatacc aacagtaacc aacctcagtg tgggtataag
     2941 aaaccaccta tgacctgctt ggtgctgatt tgtgaaccca ttcctcaccc atcaaatatt
     3001 gaaattcctt tagatagcaa gactttcctc agtcgacaca gcctggatat gaaattttct
     3061 tattgtgatg aaagaattac cgaattgatg ggatatgagc cagaagaact tttaggccgc
     3121 tcaatttatg aatattatca tgctttggac tctgatcatc tgaccaaaac tcatcatgat
     3181 atgtttacta aaggacaagt caccacagga cagtacagga tgcttgccaa aagaggtgga
     3241 tatgtctggg ttgaaactca agcaactgtc atatataaca ccaagaattc tcaaccacag
     3301 tgcattgtat gtgtgaatta cgttgtgagt ggtattattc agcacgactt gattttctcc
     3361 cttcaacaaa cagaatgtgt ccttaaaccg gttgaatctt cagatatgaa aatgactcag
     3421 ctattcacca aagttgaatc agaagataca agtagcctct ttgacaaact taagaaggaa
     3481 cctgatgctt taactttgct ggccccagcc gctggagaca caatcatatc tttagatttt
     3541 ggcagcaacg acacagaaac tgatgaccag caacttgagg aagtaccatt atataatgat
     3601 gtaatgctcc cctcacccaa cgaaaaatta cagaatataa atttggcaat gtctccatta
     3661 cccaccgctg aaacgccaaa gccacttcga agtagtgctg accctgcact caatcaagaa
     3721 gttgcattaa aattagaacc aaatccagag tcactggaac tttcttttac catgccccag
     3781 attcaggatc agacacctag tccttccgat ggaagcacta gacaaagttc acctgagcct
     3841 aatagtccca gtgaatattg tttttatgtg gatagtgata tggtcaatga attcaagttg
     3901 gaattggtag aaaaactttt tgctgaagac acagaagcaa agaacccatt ttctactcag
     3961 gacacagatt tagacttgga gatgttagct ccctatatcc caatggatga tgacttccag
     4021 ttacgttcct tcgatcagtt gtcaccatta gaaagcagtt ccgcaagccc tgaaagcgca
     4081 agtcctcaaa gcacagttac agtattccag cagactcaaa tacaagaacc tactgctaat
     4141 gccaccacta ccactgccac cactgatgaa ttaaaaacag tgacaaaaga ccgtatggaa
     4201 gacattaaaa tattgattgc atctccatct cctacccaca tacataaaga aactactagt
     4261 gccacatcat caccatatag agatactcaa agtcggacag cctcaccaaa cagagcagga
     4321 aaaggagtca tagaacagac agaaaaatct catccaagaa gccctaacgt gttatctgtc
     4381 gctttgagtc aaagaactac agttcctgag gaagaactaa atccaaagat actagctttg
     4441 cagaatgctc agagaaagcg aaaaatggaa catgatggtt cactttttca agcagtagga
     4501 attggaacat tattacagca gccagacgat catgcagcta ctacatcact ttcttggaaa
     4561 cgtgtaaaag gatgcaaatc tagtgaacag aatggaatgg agcaaaagac aattatttta
     4621 ataccctctg atttagcatg tagactgctg gggcaatcaa tggatgaaag tggattacca
     4681 cagctgacca gttatgattg tgaagttaat gctcctatac aaggcagcag aaacctactg
     4741 cagggtgaag aattactcag agctttggat caagttaacc tcgagggtgg aggcggatca
     4801 ggaggcggtg gatctatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     4861 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     4921 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     4981 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     5041 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     5101 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     5161 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     5221 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     5281 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     5341 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     5401 ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag
     5461 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     5521 gagctgtaca agtaggatcc gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     5581 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     5641 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     5701 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     5761 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     5821 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     5881 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     5941 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     6001 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     6061 cgccgttaca gatccaagct gtgaccggcg cctacgctag gccaccatgg ctgaacaaga
     6121 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     6181 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     6241 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     6301 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     6361 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     6421 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     6481 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     6541 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     6601 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     6661 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     6721 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     6781 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     6841 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     6901 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6961 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     7021 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     7081 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     7141 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7201 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     7261 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     7321 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7381 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7441 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     7501 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7561 aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta
     7621 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7681 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7741 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7801 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7861 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7921 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7981 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc
     8041 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8101 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg
     8161 cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8221 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8281 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8341 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8401 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8461 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8521 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8581 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8641 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8701 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8761 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8821 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8881 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8941 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9001 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     9061 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9121 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9181 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9241 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9301 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9361 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9421 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9481 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9541 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9601 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9661 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9721 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9781 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9841 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9901 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9961 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10021 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10081 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10141 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10201 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10261 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10321 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    10381 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    10441 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    10501 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    10561 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    10621 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    10681 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    10741 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    10801 cagtgccaag ctg
//
