LOCUS       V043892                15205 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043892
VERSION     V043892
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..9834
                     /label="FASN(NM_004104)"
                     /note="FASN(NM_004104)"
                     /gene="FASN"
     CDS             9841..9930
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        9974..10185
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             10198..10466
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             10499..11293
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    11300..11888
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             11962..12195
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    12267..12388
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      12407..12542
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(12580..12596)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    12604..12620
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(12628..12658)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    12673..12694
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(12982..13570)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(13741..14601)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(14602..14706)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     15180..15196
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggaggag gtggtgattg ccggcatgtc cgggaagctg
     2341 ccagagtcgg agaacttgca ggagttctgg gacaacctca tcggcggtgt ggacatggtc
     2401 acggacgatg accgtcgctg gaaggcgggg ctctacggcc tgccccggcg gtccggcaag
     2461 ctgaaggacc tgtctaggtt tgatgcctcc ttcttcggag tccaccccaa gcaggcacac
     2521 acgatggacc ctcagctgcg gctgctgctg gaagtcacct atgaagccat cgtggacgga
     2581 ggcatcaacc cagattcact ccgaggaaca cacactggcg tctgggtggg cgtgagcggc
     2641 tctgagacct cggaggccct gagccgagac cccgagacac tcgtgggcta cagcatggtg
     2701 ggctgccagc gagcgatgat ggccaaccgg ctctccttct tcttcgactt cagagggccc
     2761 agcatcgcac tggacacagc ctgctcctcc agcctgatgg ccctgcagaa cgcctaccag
     2821 gccatccaca gcgggcagtg ccctgccgcc atcgtggggg gcatcaatgt cctgctgaag
     2881 cccaacacct ccgtgcagtt cttgaggctg gggatgctca gccccgaggg cacctgcaag
     2941 gccttcgaca cagcggggaa tgggtactgc cgctcggagg gtgtggtggc cgtcctgctg
     3001 accaagaagt ccctggcccg gcgggtgtac gccaccatcc tgaacgccgg caccaataca
     3061 gatggcttca aggagcaagg cgtgaccttc ccctcagggg atatccagga gcagctcatc
     3121 cgctcgttgt accagtcggc cggagtggcc cctgagtcat ttgaatacat cgaagcccac
     3181 ggcacaggca ccaaggtggg cgacccccag gagctgaatg gcatcacccg agccctgtgc
     3241 gccacccgcc aggagccgct gctcatcggc tccaccaagt ccaacatggg gcacccggag
     3301 ccagcctcgg ggctggcagc cctggccaag gtgctgctgt ccctggagca cgggctctgg
     3361 gcccccaacc tgcacttcca tagccccaac cctgagatcc cagcgctgtt ggatgggcgg
     3421 ctgcaggtgg tggaccagcc cctgcccgtc cgtggcggca acgtgggcat caactccttt
     3481 ggcttcgggg gctccaacgt gcacatcatc ctgaggccca acacgcagcc gccccccgca
     3541 cccgccccac atgccaccct gccccgtctg ctgcgggcca gcggacgcac ccctgaggcc
     3601 gtgcagaagc tgctggagca gggcctccgg cacagccagg acctggcttt cctgagcatg
     3661 ctgaacgaca tcgcggctgt ccccgccacc gccatgccct tccgtggcta cgctgtgctg
     3721 ggtggtgagc gcggtggccc agaggtgcag caggtgcccg ctggcgagcg cccgctctgg
     3781 ttcatctgct ctgggatggg cacacagtgg cgcgggatgg ggctgagcct catgcgcctg
     3841 gaccgcttcc gagattccat cctacgctcc gatgaggctg tgaagccatt cggcctgaag
     3901 gtgtcacagc tgctgctgag cacagacgag agcacctttg atgacatcgt ccattcgttt
     3961 gtgagcctga ctgccatcca gataggcctc atagacctgc tgagctgcat ggggctgagg
     4021 ccagatggca tcgtcggcca ctccctgggg gaggtggcct gtggctacgc cgacggctgc
     4081 ctgtcccagg aggaggccgt cctcgctgcc tactggaggg gacagtgcat caaagaagcc
     4141 catctcccgc cgggcgccat ggcagccgtg ggcttgtcct gggaggagtg taaacagcgc
     4201 tgccccccgg gcgtggtgcc cgcctgccac aactccaagg acacagtcac catctcggga
     4261 cctcaggccc cggtgtttga gttcgtggag cagctgagga aggagggtgt gtttgccaag
     4321 gaggtgcgga ccggcggtat ggccttccac tcctacttca tggaggccat cgcaccccca
     4381 ctgctgcagg agctcaagaa ggtgatccgg gagccgaagc cacgttcagc ccgctggctc
     4441 agcacctcta tccccgaggc ccagtggcac agcagcctgg cacgcacgtc ctccgccgag
     4501 tacaatgtca acaacctggt gagccctgtg ctgttccagg aggccctgtg gcacgtgcct
     4561 gagcacgcgg tggtgctgga gatcgcgccc cacgccctgc tgcaggctgt cctgaagcgt
     4621 ggcctgaagc cgagctgcac catcatcccc ctgatgaaga aggatcacag ggacaacctg
     4681 gagttcttcc tggccggcat cggcaggctg cacctctcag gcatcgacgc caaccccaat
     4741 gccttgttcc cacctgtgga gttcccagct ccccgaggaa ctcccctcat ctccccactc
     4801 atcaagtggg accacagcct ggcctgggac gtgccggccg ccgaggactt ccccaacggt
     4861 tcaggttccc cctcagccgc catctacaac atcgacacca gctccgagtc tcctgaccac
     4921 tacctggtgg accacaccct cgacggtcgc gtcctcttcc ccgccactgg ctacctgagc
     4981 atagtgtgga agacgctggc ccgcgccctg ggcctgggcg tcgagcagct gcctgtggtg
     5041 tttgaggatg tggtgctgca ccaggccacc atcctgccca agactgggac agtgtccctg
     5101 gaggtacggc tcctggaggc ctcccgtgcc ttcgaggtgt cagagaacgg caacctggta
     5161 gtgagtggga aggtgtacca gtgggatgac cctgacccca ggctcttcga ccacccggaa
     5221 agccccaccc ccaaccccac ggagcccctc ttcctggccc aggctgaagt ttacaaggag
     5281 ctgcgtctgc gtggctacga ctacggccct catttccagg gcatcctgga ggccagcctg
     5341 gaaggtgact cggggaggct gctgtggaag gataactggg tgagcttcat ggacaccatg
     5401 ctgcagatgt ccatcctggg ctcggccaag cacggcctgt acctgcccac ccgtgtcacc
     5461 gccatccaca tcgaccctgc cacccacagg cagaagctgt acacactgca ggacaaggcc
     5521 caagtggctg acgtggtggt gagcaggtgg ctgagggtca cagtggccgg aggcgtccac
     5581 atctccgggc tccacactga gtcggccccg cggcggcagc aggagcagca ggtgcccatc
     5641 ctggagaagt tttgcttcac tccccacacg gaggaggggt gcctgtctga gcgcgctgcc
     5701 ctgcaggagg agctgcaact gtgcaagggg ctggtgcagg cactgcagac caaggtgacc
     5761 cagcaggggc tgaagatggt ggtgcccgga ctggatgggg cccagatccc ccgggacccc
     5821 tcacagcagg aactgccccg gctgttgtcg gctgcctgca ggcttcagct caacgggaac
     5881 ctgcagctgg agctggcgca ggtgctggcc caggagaggc ccaagctgcc agaggaccct
     5941 ctgctcagcg gcctcctgga ctccccggca ctcaaggcct gcctggacac tgccgtggag
     6001 aacatgccca gcctgaagat gaaggtggtg gaggtgctgg ctggccacgg tcacctgtat
     6061 tcccgcatcc caggcctgct cagcccccat cccctgctgc agctgagcta cacggccacc
     6121 gaccgccacc cccaggccct ggaggctgcc caggccgagc tgcagcagca cgacgttgcc
     6181 cagggccagt gggatcccgc agaccctgcc cccagcgccc tgggcagcgc cgacctcctg
     6241 gtgtgcaact gtgctgtggc tgccctcggg gacccggcct cagctctcag caacatggtg
     6301 gctgccctga gagaaggggg ctttctgctc ctgcacacac tgctccgggg gcaccccctc
     6361 ggggacatcg tggccttcct cacctccact gagccgcagt atggccaggg catcctgagc
     6421 caggacgcgt gggagagcct cttctccagg gtgtcgctgc gcctggtggg cctgaagaag
     6481 tccttctacg gctccacgct cttcctgtgc cgccggccca ccccgcagga cagccccatc
     6541 ttcctgccgg tggacgatac cagcttccgc tgggtggagt ctctgaaggg catcctggct
     6601 gacgaagact cttcccggcc tgtgtggctg aaggccatca actgtgccac ctcgggcgtg
     6661 gtgggcttgg tgaactgtct ccgccgagag cccggcggga accgcctccg gtgtgtgctg
     6721 ctctccaacc tcagcagcac ctcccacgtc ccggaggtgg acccgggctc cgcagaactg
     6781 cagaaggtgt tgcagggaga cctggtgatg aacgtctacc gcgacggggc ctggggggct
     6841 ttccgccact tcctgctgga ggaggacaag cctgaggagc cgacggcaca tgcctttgtg
     6901 agcaccctca cccgggggga cctgtcctcc atccgctggg tctgctcctc gctgcgccat
     6961 gcccagccca cctgccctgg cgcccagctc tgcacggtct actacgcctc cctcaacttc
     7021 cgcgacatca tgctggccac tggcaagctg tcccctgatg ccatcccagg gaagtggacc
     7081 tcccaggaca gcctgctagg tatggagttc tcgggccgag acgccagcgg caagcgtgtg
     7141 atgggactgg tgcctgccaa gggcctggcc acctctgtcc tgctgtcacc ggacttcctc
     7201 tgggatgtgc cttccaactg gacgctggag gaggcggcct cggtgcctgt cgtctacagc
     7261 acggcctact acgcgctggt ggtgcgtggg cgggtgcgcc ccggggagac gctgctcatc
     7321 cactcgggct cgggcggcgt gggccaggcc gccatcgcca tcgccctcag tctgggctgc
     7381 cgcgtcttca ccaccgtggg gtcggctgag aagcgggcgt acctccaggc caggttcccc
     7441 cagctcgaca gcaccagctt cgccaactcc cgggacacat ccttcgagca gcatgtgctg
     7501 tggcacacgg gcgggaaggg cgttgacctg gtcttgaact ccttggcgga agagaagctg
     7561 caggccagcg tgaggtgctt ggctacgcac ggtcgcttcc tggaaattgg caaattcgac
     7621 ctttctcaga accacccgct cggcatggct atcttcctga agaacgtgac attccacggg
     7681 gtcctactgg atgcgttctt caacgagagc agtgctgact ggcgggaggt gtgggcgctt
     7741 gtgcaggccg gcatccggga tggggtggta cggcccctca agtgcacggt gttccatggg
     7801 gcccaggtgg aggacgcctt ccgctacatg gcccaaggga agcacattgg caaagtcgtc
     7861 gtgcaggtgc ttgcggagga gccggaggca gtgctgaagg gggccaaacc caagctgatg
     7921 tcggccatct ccaagacctt ctgcccggcc cacaagagct acatcatcgc tggtggtctg
     7981 ggtggcttcg gcctggagtt ggcgcagtgg ctgatacagc gtggggtgca gaagctcgtg
     8041 ttgacttctc gctccgggat ccggacaggc taccaggcca agcaggtccg ccggtggagg
     8101 cgccagggcg tacaggtgca ggtgtccacc agcaacatca gctcactgga gggggcccgg
     8161 ggcctcattg ccgaggcggc gcagcttggg cccgtgggcg gcgtcttcaa cctggccgtg
     8221 gtcttgagag atggcttgct ggagaaccag accccagagt tcttccagga cgtctgcaag
     8281 cccaagtaca gcggcaccct gaacctggac agggtgaccc gagaggcgtg ccctgagctg
     8341 gactactttg tggtcttctc ctctgtgagc tgcgggcgtg gcaatgcggg acagagcaac
     8401 tacggctttg ccaattccgc catggagcgt atctgtgaga aacgccggca cgaaggcctc
     8461 ccaggcctgg ccgtgcagtg gggcgccatc ggcgacgtgg gcattttggt ggagacgatg
     8521 agcaccaacg acacgatcgt cagtggcacg ctgccccagc gcatggcgtc ctgcctggag
     8581 gtgctggacc tcttcctgaa ccagccccac atggtcctga gcagctttgt gctggctgag
     8641 aaggctgcgg cctataggga cagggacagc cagcgggacc tggtggaggc cgtggcacac
     8701 atcctgggca tccgcgactt ggctgctgtc aacctggaca gctcactggc ggacctgggc
     8761 ctggactcgc tcatgagcgt ggaggtgcgc cagacgctgg agcgtgagct caacctggtg
     8821 ctgtccgtgc gcgaggtgcg gcaactcacg ctccggaaac tgcaggagct gtcctcaaag
     8881 gcggatgagg ccagcgagct ggcatgcccc acgcccaagg aggatggtct ggcccagcag
     8941 cagactcagc tgaacctgcg ctccctgctg gtgaacccgg agggccccac cctgatgcgg
     9001 ctcaactccg tgcagagctc ggagcggccc ctgttcctgg tgcacccaat cgagggctcc
     9061 accaccgtgt tccacagcct ggcctcccgg ctcagcatcc ccacctatgg cctgcagtgc
     9121 acccgagctg cgccccttga cagcatccac agcctggctg cctactacat cgactgcatc
     9181 aggcaggtgc agcccgaggg cccctaccgc gtggccggct actcctacgg ggcctgcgtg
     9241 gcctttgaaa tgtgctccca gctgcaggcc cagcagagcc cagcccccac ccacaacagc
     9301 ctcttcctgt tcgacggctc gcccacctac gtactggcct acacccagag ctaccgggca
     9361 aagctgaccc caggctgtga ggctgaggct gagacggagg ccatatgctt cttcgtgcag
     9421 cagttcacgg acatggagca caacagggtg ctggaggcgc tgctgccgct gaagggccta
     9481 gaggagcgtg tggcagccgc cgtggacctg atcatcaaga gccaccaggg cctggaccgc
     9541 caggagctga gctttgcggc ccggtccttc tactacaagc tgcgtgccgc tgagcagtac
     9601 acacccaagg ccaagtacca tggcaacgtg atgctactgc gcgccaagac gggtggcgcc
     9661 tacggcgagg acctgggcgc ggactacaac ctctcccagg tatgcgacgg gaaagtatcc
     9721 gtccacgtca tcgagggtga ccaccgcacg ctgctggagg gcagcggcct ggagtccatc
     9781 atcagcatca tccacagctc cctggctgag ccacgcgtga gcgtgcggga gggcggatcc
     9841 gaacaaaaac taatatcaga ggaagatttg gaacagaaac taatcagtga agaagattta
     9901 gagcaaaagt taatttccga ggaggactta tagcggccgc gaaggatctg cgatcgctcc
     9961 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
    10021 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
    10081 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
    10141 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
    10201 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
    10261 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
    10321 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
    10381 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
    10441 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct aggccaccat
    10501 ggctgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
    10561 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
    10621 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
    10681 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
    10741 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
    10801 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
    10861 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
    10921 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
    10981 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
    11041 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
    11101 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
    11161 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
    11221 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
    11281 cgagttcttc tgagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
    11341 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
    11401 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
    11461 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
    11521 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
    11581 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
    11641 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
    11701 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
    11761 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
    11821 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
    11881 ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
    11941 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc
    12001 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
    12061 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
    12121 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
    12181 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
    12241 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
    12301 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    12361 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
    12421 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
    12481 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    12541 cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg
    12601 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
    12661 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
    12721 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
    12781 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    12841 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    12901 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    12961 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    13021 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    13081 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    13141 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    13201 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    13261 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    13321 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    13381 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
    13441 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    13501 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    13561 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    13621 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    13681 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    13741 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    13801 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    13861 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    13921 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    13981 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    14041 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    14101 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    14161 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    14221 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    14281 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    14341 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    14401 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    14461 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    14521 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    14581 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    14641 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    14701 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
    14761 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
    14821 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    14881 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
    14941 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
    15001 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
    15061 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
    15121 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    15181 taaaacgacg gccagtgcca agctg
//
