LOCUS       V046138                10693 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046138
VERSION     V046138
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5325
                     /label="DHX36(NM_020865)"
                     /note="DHX36(NM_020865)"
                     /gene="DHX36"
     CDS             5332..5358
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5362..5388
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5392..5418
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5462..5673
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5686..5954
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5987..6781
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6788..7376
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7450..7683
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7755..7876
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7895..8030
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8068..8084)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8092..8108
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8116..8146)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8161..8182
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8470..9058)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9229..10089)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10090..10194)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10668..10684
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgagttat gactaccatc agaactgggg ccgtgatggg
     2341 ggtccccgca gctccggtgg gggctatgga ggggggccag cagggggtca tggaggtaac
     2401 cgaggctccg gaggaggcgg cggcggcgga gggggtggtc gaggcggcag gggccggcat
     2461 cccgggcacc tgaaaggccg cgaaatcggc atgtggtacg cgaaaaaaca ggggcagaag
     2521 aacaaggaag cggagaggca agagagagct gtagtacaca tggatgaacg acgagaagaa
     2581 caaattgtac agttactgaa ttctgttcaa gcgaagaatg ataaagagtc agaagcacag
     2641 atatcctggt ttgctcctga ggatcatgga tacggtactg aagtttctac taagaacaca
     2701 ccatgctcag agaacaaact tgacatccag gaaaagaagt tgataaatca agaaaaaaaa
     2761 atgtttagaa tcaggaacag atcatatatt gaccgagatt ctgagtatct cttgcaagaa
     2821 aatgaaccag atggaacttt agaccaaaaa ttattggaag atttacaaaa gaaaaaaaat
     2881 gaccttcggt atattgaaat gcagcatttc agagaaaagc tgccttcgta tggaatgcaa
     2941 aaggaattgg taaatttaat tgataaccat caggtaacag taataagtgg tgaaactggt
     3001 tgtggcaaaa ccactcaagt tactcagttc attttggata actacattga aagaggaaaa
     3061 ggatctgctt gcagaatagt ttgtactcag ccaagaagaa ttagtgccat ttcagttgcg
     3121 gaaagagtag ctgcagaaag ggcagaatct tgtggcagtg gtaatagtac tggatatcaa
     3181 attcgtctcc agagtcggtt gccaaggaaa cagggttcta tcttatactg tacaacagga
     3241 atcatccttc agtggctcca gtcagacccg tatttgtcca gtgttagtca tatcgtactt
     3301 gatgaaatcc atgaaagaaa tctgcagtca gatgttttaa tgactgttgt taaagacctt
     3361 ctcaattttc gatctgactt gaaagtaata ttgatgagtg caacattgaa tgcagaaaag
     3421 ttttcagaat attttggtaa ctgtccaatg atacatatac ctggttttac ctttccggtt
     3481 gtggaatatc ttttggaaga tgtaattgaa aaaataaggt atgttccaga acaaaaagaa
     3541 cacagatccc agtttaagag gggtttcatg caagggcatg taaatagaca agaaaaagaa
     3601 gaaaaagaag caatatataa agaacgttgg ccagattatg taagggaact gcgaagaagg
     3661 tattctgcaa gtactgtaga tgttatagaa atgatggagg atgataaagt tgatctgaat
     3721 ttgattgttg ccctcatccg atacattgtt ttggaagaag aggatggtgc gatactggtc
     3781 tttctgccag gctgggacaa tatcagcact ttacatgatc tcttgatgtc acaagtaatg
     3841 tttaaatcag ataaattttt aattatacct ttacattcac tgatgcctac agttaaccag
     3901 acacaggtgt ttaaaagaac ccctcctggt gttcggaaaa tagtaattgc taccaacatt
     3961 gcggagacta gcattaccat agatgatgtc gtttatgtga tagatggagg aaaaataaaa
     4021 gagacgcatt ttgatactca gaacaatatc agtacaatgt ccgctgagtg ggttagtaaa
     4081 gctaatgcca aacagagaaa aggtcgagct ggaagagttc aacctggtca ttgctatcat
     4141 ctgtataatg gtcttagagc aagtcttcta gatgactatc aactgccaga aattttgaga
     4201 actcctttgg aagaactttg tttacaaata aagattttaa ggctaggtgg aattgcttat
     4261 tttctgagta gattaatgga cccaccatca aatgaggcag tgttactctc cataagacac
     4321 ctgatggagc tgaacgcttt ggataaacaa gaagaattga cacctcttgg agtccacttg
     4381 gcacgattac ccgttgagcc acatattgga aaaatgattc tttttggagc actgttctgc
     4441 tgcttagacc cagtactcac tattgctgct agtctcagtt tcaaagatcc atttgtcatt
     4501 ccactgggaa aagaaaagat tgcagatgca agaagaaagg aattggcaaa ggatactaga
     4561 agtgatcact taacagttgt gaatgcgttt gagggctggg aagaggctag gcgacgtggt
     4621 ttcagatacg aaaaggacta ttgctgggaa tattttctgt cttcaaacac actgcagatg
     4681 ctgcataaca tgaaaggaca gtttgctgag catcttcttg gagctggatt tgtaagcagt
     4741 agaaatccta aagatccaga atctaatata aattcagata atgagaagat aattaaagct
     4801 gtcatctgtg ctggtttata tcccaaagtt gctaaaattc gactaaattt gggtaaaaaa
     4861 agaaaaatgg taaaagttta cacaaaaacc gatggcctgg ttgctgttca tcctaaatct
     4921 gttaatgtgg agcaaacaga ctttcactac aactggctta tctatcacct aaagatgaga
     4981 acaagcagta tatacttgta tgactgcaca gaggtttccc catactgtct cttgtttttt
     5041 ggaggtgaca tttccatcca gaaggataac gatcaggaaa ctattgctgt agatgagtgg
     5101 attgtatttc agtctccagc aagaattgcc catcttgtta aggaattaag aaaggaacta
     5161 gatattcttc tgcaagagaa gattgaaagt cctcatcctg tagactggaa tgacactaaa
     5221 tccagagact gtgcagtact gtcagctatt atagacttga tcaaaacaca ggaaaaggca
     5281 actcccagga actttccgcc acgattccag gatggatatt acagcggatc ctacccatac
     5341 gacgtacctg attatgcaac ttatccttat gatgtacctg attatgctag ctatccttat
     5401 gatgtcccag actacgcata gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     5461 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     5521 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     5581 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     5641 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     5701 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     5761 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     5821 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     5881 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     5941 cgccgttaca gatccaagct gtgaccggcg cctacgctag gccaccatgg ctgaacaaga
     6001 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     6061 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     6121 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     6181 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     6241 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     6301 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     6361 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     6421 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     6481 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     6541 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     6601 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     6661 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     6721 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     6781 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6841 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6901 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6961 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     7021 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7081 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     7141 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     7201 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7261 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7321 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     7381 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7441 aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta
     7501 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7561 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7621 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7681 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7741 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7801 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7861 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc
     7921 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7981 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg
     8041 cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8101 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8161 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8221 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8281 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8341 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8401 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8461 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8521 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8581 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8641 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8701 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8761 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8821 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8881 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     8941 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9001 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9061 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9121 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9181 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9241 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9301 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9361 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9421 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9481 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9541 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9601 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9661 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9721 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9781 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9841 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9901 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9961 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10021 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10081 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10141 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10201 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    10261 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    10321 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    10381 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    10441 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    10501 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    10561 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    10621 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    10681 cagtgccaag ctg
//
