LOCUS       V023544                11060 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023544
VERSION     V023544
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4897
                     /label="PROM1(NM_006017)"
                     /note="PROM1(NM_006017)"
                     /gene="PROM1"
     CDS             4904..4969
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5013..5224
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5237..5505
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5534..5543
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5564..6226
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6296..6349
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6356..7150
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    7157..7745
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7817..8050
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8122..8243
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8262..8397
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8435..8451)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8459..8475
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8483..8513)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8528..8549
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8837..9425)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9596..10456)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10457..10561)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11035..11051
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggccct cgtactcggc tccctgttgc tgctggggct
     2341 gtgcgggaac tccttttcag gagggcagcc ttcatccaca gatgctccta aggcttggaa
     2401 ttatgaattg cctgcaacaa attatgagac ccaagactcc cataaagctg gacccattgg
     2461 cattctcttt gaactagtgc atatctttct ctatgtggta cagccgcgtg atttcccaga
     2521 agatactttg agaaaattct tacagaaggc atatgaatcc aaaattgatt atgacaagcc
     2581 agaaactgta atcttaggtc taaagattgt ctactatgaa gcagggatta ttctatgctg
     2641 tgtcctgggg ctgctgttta ttattctgat gcctctggtg gggtatttct tttgtatgtg
     2701 tcgttgctgt aacaaatgtg gtggagaaat gcaccagcga cagaaggaaa atgggccctt
     2761 cctgaggaaa tgctttgcaa tctccctgtt ggtgatttgt ataataataa gcattggcat
     2821 cttctatggt tttgtggcaa atcaccaggt aagaacccgg atcaaaagga gtcggaaact
     2881 ggcagatagc aatttcaagg acttgcgaac tctcttgaat gaaactccag agcaaatcaa
     2941 atatatattg gcccagtaca acactaccaa ggacaaggcg ttcacagatc tgaacagtat
     3001 caattcagtg ctaggaggcg gaattcttga ccgactgaga cccaacatca tccctgttct
     3061 tgatgagatt aagtccatgg caacagcgat caaggagacc aaagaggcgt tggagaacat
     3121 gaacagcacc ttgaagagct tgcaccaaca aagtacacag cttagcagca gtctgaccag
     3181 cgtgaaaact agcctgcggt catctctcaa tgaccctctg tgcttggtgc atccatcaag
     3241 tgaaacctgc aacagcatca gattgtctct aagccagctg aatagcaacc ctgaactgag
     3301 gcagcttcca cccgtggatg cagaacttga caacgttaat aacgttctta ggacagattt
     3361 ggatggcctg gtccaacagg gctatcaatc ccttaatgat atacctgaca gagtacaacg
     3421 ccaaaccacg actgtcgtag caggtatcaa aagggtcttg aattccattg gttcagatat
     3481 cgacaatgta actcagcgtc ttcctattca ggatatactc tcagcattct ctgtttatgt
     3541 taataacact gaaagttaca tccacagaaa tttacctaca ttggaagagt atgattcata
     3601 ctggtggctg ggtggcctgg tcatctgctc tctgctgacc ctcatcgtga ttttttacta
     3661 cctgggctta ctgtgtggcg tgtgcggcta tgacaggcat gccaccccga ccacccgagg
     3721 ctgtgtctcc aacaccggag gcgtcttcct catggttgga gttggattaa gtttcctctt
     3781 ttgctggata ttgatgatca ttgtggttct tacctttgtc tttggtgcaa atgtggaaaa
     3841 actgatctgt gaaccttaca cgagcaagga attattccgg gttttggata caccctactt
     3901 actaaatgaa gactgggaat actatctctc tgggaagcta tttaataaat caaaaatgaa
     3961 gctcactttt gaacaagttt acagtgactg caaaaaaaat agaggcactt acggcactct
     4021 tcacctgcag aacagcttca atatcagtga acatctcaac attaatgagc atactggaag
     4081 cataagcagt gaattggaaa gtctgaaggt aaatcttaat atctttctgt tgggtgcagc
     4141 aggaagaaaa aaccttcagg attttgctgc ttgtggaata gacagaatga attatgacag
     4201 ctacttggct cagactggta aatcccccgc aggagtgaat cttttatcat ttgcatatga
     4261 tctagaagca aaagcaaaca gtttgccccc aggaaatttg aggaactccc tgaaaagaga
     4321 tgcacaaact attaaaacaa ttcaccagca acgagtcctt cctatagaac aatcactgag
     4381 cactctatac caaagcgtca agatacttca acgcacaggg aatggattgt tggagagagt
     4441 aactaggatt ctagcttctc tggattttgc tcagaacttc atcacaaaca atacttcctc
     4501 tgttattatt gaggaaacta agaagtatgg gagaacaata ataggatatt ttgaacatta
     4561 tctgcagtgg atcgagttct ctatcagtga gaaagtggca tcgtgcaaac ctgtggccac
     4621 cgctctagat actgctgttg atgtctttct gtgtagctac attatcgacc ccttgaattt
     4681 gttttggttt ggcataggaa aagctactgt atttttactt ccggctctaa tttttgcggt
     4741 aaaactggct aagtactatc gtcgaatgga ttcggaggac gtgtacgatg atgttgaaac
     4801 tatacccatg aaaaatatgg aaaatggtaa taatggttat cataaagatc atgtatatgg
     4861 tattcacaat cctgttatga caagcccatc acaacatgga tccgactaca aagaccatga
     4921 cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt agcggccgcg
     4981 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     5041 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
     5101 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     5161 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     5221 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
     5281 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
     5341 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
     5401 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
     5461 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc
     5521 gcctacgcta gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga
     5581 gtgccgcatc accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg
     5641 cacccccaag cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt
     5701 cagcccctac ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc
     5761 cagcggctac gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg
     5821 catcgagaag tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc
     5881 cggccgcgtg atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat
     5941 cttcaccgac aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga
     6001 taacgtgctg gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag
     6061 cttcgtggtg gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa
     6121 cgggggcccc atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg
     6181 catcgtggag taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca
     6241 gtcgtccaat tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg
     6301 cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggccctg ccaccatggc
     6361 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     6421 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     6481 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     6541 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     6601 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     6661 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     6721 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     6781 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     6841 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     6901 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     6961 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     7021 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     7081 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     7141 gttcttctga gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     7201 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7261 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7321 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     7381 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7441 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7501 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     7561 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7621 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7681 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7741 cccgcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     7801 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga tctgcttttt
     7861 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7921 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7981 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8041 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     8101 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     8161 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8221 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     8281 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     8341 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     8401 acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     8461 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     8521 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     8581 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     8641 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8701 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8761 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8821 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8881 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8941 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9001 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     9061 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     9121 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     9181 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     9241 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     9301 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9361 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9421 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     9481 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     9541 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9601 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9661 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9721 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9781 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9841 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9901 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9961 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    10021 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    10081 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    10141 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    10201 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    10261 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    10321 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    10381 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    10441 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    10501 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    10561 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
    10621 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
    10681 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
    10741 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
    10801 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
    10861 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
    10921 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
    10981 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
    11041 cgacggccag tgccaagctg
//
