LOCUS       V041413                11783 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041413
VERSION     V041413
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..5605
                     /label="ACLY(NM_001096)"
                     /note="ACLY(NM_001096)"
                     /gene="ACLY"
     CDS             5606..5632
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5636..5662
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5666..5692
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5736..5947
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5960..6228
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6257..6266
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6287..6949
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7019..7072
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7079..7873
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    7880..8468
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8540..8773
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8845..8966
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8985..9120
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9158..9174)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9182..9198
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9206..9236)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9251..9272
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9560..10148)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10319..11179)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11180..11284)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11758..11774
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgtcggc caaggcaatt tcagagcaga cgggcaaaga
     2341 actcctttac aagttcatct gtaccacctc agccatccag aatcggttca agtatgctcg
     2401 ggtcactcct gacacagact gggcccgctt gctgcaggac cacccctggc tgctcagcca
     2461 gaacttggta gtcaagccag accagctgat caaacgtcgt ggaaaacttg gtctcgttgg
     2521 ggtcaacctc actctggatg gggtcaagtc ctggctgaag ccacggctgg gacaggaagc
     2581 cacagttggc aaggccacag gcttcctcaa gaactttctg atcgagccct tcgtccccca
     2641 cagtcaggct gaggagttct atgtctgcat ctatgccacc cgagaagggg actacgtcct
     2701 gttccaccac gaggggggtg tggacgtggg tgatgtggac gccaaggccc agaagctgct
     2761 tgttggcgtg gatgagaaac tgaatcctga ggacatcaaa aaacacctgt tggtccacgc
     2821 ccctgaagac aagaaagaaa ttctggccag ttttatctcc ggcctcttca atttctacga
     2881 ggacttgtac ttcacctacc tcgagatcaa tccccttgta gtgaccaaag atggagtcta
     2941 tgtccttgac ttggcggcca aggtggacgc cactgccgac tacatctgca aagtgaagtg
     3001 gggtgacatc gagttccctc cccccttcgg gcgggaggca tatccagagg aagcctacat
     3061 tgcagacctc gatgccaaaa gtggggcaag cctgaagctg accttgctga accccaaagg
     3121 gaggatctgg accatggtgg ccgggggtgg cgcctctgtc gtgtacagcg ataccatctg
     3181 tgatctaggg ggtgtcaacg agctggcaaa ctatggggag tactcaggcg cccccagcga
     3241 gcagcagacc tatgactatg ccaagactat cctctccctc atgacccgag agaagcaccc
     3301 agatggcaag atcctcatca ttggaggcag catcgcaaac ttcaccaacg tggctgccac
     3361 gttcaagggc atcgtgagag caattcgaga ttaccagggc cccctgaagg agcacgaagt
     3421 cacaatcttt gtccgaagag gtggccccaa ctatcaggag ggcttacggg tgatgggaga
     3481 agtcgggaag accactggga tccccatcca tgtctttggc acagagactc acatgacggc
     3541 cattgtgggc atggccctgg gccaccggcc catccccaac cagccaccca cagcggccca
     3601 cactgcaaac ttcctcctca acgccagcgg gagcacatcg acgccagccc ccagcaggac
     3661 agcatctttt tctgagtcca gggccgatga ggtggcgcct gcaaagaagg ccaagcctgc
     3721 catgccacaa gattcagtcc caagtccaag atccctgcaa ggaaagagca ccaccctctt
     3781 cagccgccac accaaggcca ttgtgtgggg catgcagacc cgggccgtgc aaggcatgct
     3841 ggactttgac tatgtctgct cccgagacga gccctcagtg gctgccatgg tctacccttt
     3901 cactggggac cacaagcaga agttttactg ggggcacaaa gagatcctga tccctgtctt
     3961 caagaacatg gctgatgcca tgaggaagca tccggaggta gatgtgctca tcaactttgc
     4021 ctctctccgc tctgcctatg acagcaccat ggagaccatg aactatgccc agatccggac
     4081 catcgccatc atagctgaag gcatccctga ggccctcacg agaaagctga tcaagaaggc
     4141 ggaccagaag ggagtgacca tcatcggacc tgccactgtt ggaggcatca agcctgggtg
     4201 ctttaagatt ggcaacacag gtgggatgct ggacaacatc ctggcctcca aactgtaccg
     4261 cccaggcagc gtggcctatg tctcacgttc cggaggcatg tccaacgagc tcaacaatat
     4321 catctctcgg accacggatg gcgtctatga gggcgtggcc attggtgggg acaggtaccc
     4381 gggctccaca ttcatggatc atgtgttacg ctatcaggac actccaggag tcaaaatgat
     4441 tgtggttctt ggagagattg ggggcactga ggaatataag atttgccggg gcatcaagga
     4501 gggccgcctc actaagccca tcgtctgctg gtgcatcggg acgtgtgcca ccatgttctc
     4561 ctctgaggtc cagtttggcc atgctggagc ttgtgccaac caggcttctg aaactgcagt
     4621 agccaagaac caggctttga aggaagcagg agtgtttgtg ccccggagct ttgatgagct
     4681 tggagagatc atccagtctg tatacgaaga tctcgtggcc aatggagtca ttgtacctgc
     4741 ccaggaggtg ccgcccccaa ccgtgcccat ggactactcc tgggccaggg agcttggttt
     4801 gatccgcaaa cctgcctcgt tcatgaccag catctgcgat gagcgaggac aggagctcat
     4861 ctacgcgggc atgcccatca ctgaggtctt caaggaagag atgggcattg gcggggtcct
     4921 cggcctcctc tggttccaga aaaggttgcc taagtactct tgccagttca ttgagatgtg
     4981 tctgatggtg acagctgatc acgggccagc cgtctctgga gcccacaaca ccatcatttg
     5041 tgcgcgagct gggaaagacc tggtctccag cctcacctcg gggctgctca ccatcgggga
     5101 tcggtttggg ggtgccttgg atgcagcagc caagatgttc agtaaagcct ttgacagtgg
     5161 cattatcccc atggagtttg tgaacaagat gaagaaggaa gggaagctga tcatgggcat
     5221 tggtcaccga gtgaagtcga taaacaaccc agacatgcga gtgcagatcc tcaaagatta
     5281 cgtcaggcag cacttccctg ccactcctct gctcgattat gcactggaag tagagaagat
     5341 taccacctcg aagaagccaa atcttatcct gaatgtagat ggtctcatcg gagtcgcatt
     5401 tgtagacatg cttagaaact gtgggtcctt tactcgggag gaagctgatg aatatattga
     5461 cattggagcc ctcaatggca tctttgtgct gggaaggagt atggggttca ttggacacta
     5521 tcttgatcag aagaggctga agcaggggct gtatcgtcat ccgtgggatg atatttcata
     5581 tgttcttccg gaacacatga gcatgtaccc atacgacgta cctgattatg caacttatcc
     5641 ttatgatgta cctgattatg ctagctatcc ttatgatgtc ccagactacg catagcggcc
     5701 gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     5761 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg
     5821 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     5881 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     5941 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg
     6001 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg
     6061 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc
     6121 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc
     6181 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc
     6241 ggcgcctacg ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat
     6301 cgagtgccgc atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga
     6361 gggcaccccc aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac
     6421 cttcagcccc tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta
     6481 ccccagcggc tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac
     6541 ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga
     6601 ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt
     6661 gatcttcacc gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg
     6721 cgataacgtg ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta
     6781 cagcttcgtg gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca
     6841 gaacgggggc cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct
     6901 gggcatcgtg gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc
     6961 tcagtcgtcc aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga
     7021 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc ctgccaccat
     7081 ggctgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     7141 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     7201 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     7261 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     7321 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     7381 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     7441 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     7501 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     7561 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     7621 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     7681 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     7741 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     7801 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     7861 cgagttcttc tgagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     7921 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7981 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     8041 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     8101 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     8161 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     8221 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     8281 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     8341 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     8401 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     8461 ctccccgcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     8521 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagata agatctgctt
     8581 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8641 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8701 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8761 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     8821 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     8881 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8941 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     9001 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     9061 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     9121 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa
     9181 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     9241 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     9301 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     9361 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     9421 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     9481 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     9541 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     9601 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     9661 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     9721 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     9781 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     9841 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     9901 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     9961 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
    10021 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    10081 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    10141 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    10201 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    10261 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    10321 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    10381 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    10441 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    10501 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    10561 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    10621 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    10681 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    10741 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    10801 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    10861 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    10921 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    10981 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    11041 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    11101 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    11161 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    11221 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    11281 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
    11341 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
    11401 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
    11461 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
    11521 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
    11581 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
    11641 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
    11701 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
    11761 aaacgacggc cagtgccaag ctg
//
