LOCUS       V034961                10181 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034961
VERSION     V034961
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4182
                     /label="DTX2(NM_020892)"
                     /note="DTX2(NM_020892)"
                     /gene="DTX2"
     CDS             4189..4215
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4219..4245
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4249..4275
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4340..4551
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4564..4832
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4861..4870
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4891..5553
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5623..5676
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5677..6276
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6277..6864
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6938..7171
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7243..7364
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7383..7518
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7556..7572)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7580..7596
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7604..7634)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7649..7670
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7958..8546)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8717..9577)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9578..9682)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10156..10172
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ccatggcccc aagcccttcc
     2341 ctggtgcagg tgtacaccag ccccgcggct gtggccgtgt gggaatggca ggacgggctg
     2401 ggcacctggc acccctacag tgccaccgtc tgcagcttca tcgagcagca gtttgtccag
     2461 cagaagggcc aacgttttgg gcttgggagc ctggcccaca gcatcccctt gggccaggca
     2521 gacccctcgc tggcccctta cattattgac ctccccagct ggacccagtt ccgccaggac
     2581 accggcacca tgcgggctgt gcggagacac ctgttccccc agcactcagc ccctggccga
     2641 ggtgtcgtct gggagtggct gagcgacgat ggctcctgga ctgcctatga agccagcgtc
     2701 tgtgactatc tggagcagca ggtggccagg ggcaaccagc tcgtggactt ggcccccctg
     2761 gggtacaact acactgtcaa ctacaccacc cacacgcaga ccaacaagac ttccagcttc
     2821 tgccgcagcg tgcggcgcca agcagggccg ccttacccgg tgaccaccat catcgctccg
     2881 ccgggccaca caggcgtcgc ctgctcttgc caccagtgcc tcagtggcag cagaactggc
     2941 cccgtgtcag gccgctaccg ccactccatg accaacctcc ctgcataccc cgtcccccag
     3001 caccccccac acaggaccgc ttctgtgttt gggacccacc aggcctttgc accgtacaac
     3061 aaaccctcac tctccggggc ccggtctgcg cccaggctga acaccaccaa cgcctggggc
     3121 gcagctcctc cttccctggg gagccagccc ctctaccgct ccagcctctc ccacctggga
     3181 ccgcagcacc tgcccccagg atcctccacc tccggtgcag tcagtgcctc cctccccagc
     3241 ggtccctcaa gcagcccagg gagcgtccct gccactgtgc ccatgcagat gccaaagccc
     3301 agcagagtcc agcaggcgct cgcaggcatg acgagtgttc tgatgtcagc cattggactc
     3361 cctgtgtgtc ttagccgcgc accccagccc accagccctc ccgcctcccg tctggcttcc
     3421 aaaagtcacg gctcagttaa gagattgagg aaaatgtccg tgaaaggagc gaccccgaag
     3481 ccagagccag agccagagca ggtcataaaa aactacacgg aagagctgaa agtgccccca
     3541 gatgaggact gcatcatctg catggagaag ctgtccacag cgtctggata cagcgatgtg
     3601 actgacagca aggcaatcgg gtccctagct gtgggccacc tcaccaagtg cagccatgcc
     3661 ttccacctgc tgtgcctcct ggccatgtac tgcaacggca ataaggatgg aagtctgcag
     3721 tgtccctcct gcaaaaccat ctatggagag aagacgggga cccagcccca gggaaagatg
     3781 gaggtattac ggttccagat gtcgctcccc ggccacgagg actgcgggac catcctcata
     3841 gtttacagca ttccccatgg tatccagggc cctgagcacc ccaatcccgg aaagccgttc
     3901 actgccagag ggtttccccg ccagtgctac cttccagaca acgcccaggg ccgcaaggtc
     3961 ctagagctcc tgaaggtggc ctggaagagg cggctcatct tcacagtggg cacgtccagc
     4021 accacgggtg agacggacac cgtggtatgg aacgagatcc accacaagac agagatggac
     4081 cgcaacatta cgggccacgg ctatcccgac cccaactacc tgcagaacgt gctggctgag
     4141 ctggctgccc agggggtgac cgaggactgc ctggagcagc agctcgagta cccatacgac
     4201 gtacctgatt atgcaactta tccttatgat gtacctgatt atgctagcta tccttatgat
     4261 gtcccagact acgcataggg atccctgcga tatccagcgc ggccgcgaag gatctgcgat
     4321 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     4381 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     4441 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     4501 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
     4561 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
     4621 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
     4681 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
     4741 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
     4801 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac
     4861 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc
     4921 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag
     4981 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg
     5041 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag
     5101 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac
     5161 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc
     5221 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag
     5281 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg
     5341 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac
     5401 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg
     5461 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac
     5521 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct
     5581 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt
     5641 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg
     5701 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     5761 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     5821 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     5881 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     5941 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     6001 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     6061 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     6121 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     6181 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     6241 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt
     6301 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6361 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6421 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6481 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6541 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6601 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6661 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     6721 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6781 ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc ttcgccctca gacgagtcgg
     6841 atctcccttt ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct
     6901 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     6961 cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     7021 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7081 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7141 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     7201 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     7261 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     7321 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     7381 ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     7441 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     7501 gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat
     7561 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     7621 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7681 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     7741 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7801 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7861 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7921 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7981 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8041 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8101 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8161 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8221 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8281 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8341 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     8401 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8461 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8521 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8581 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8641 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8701 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8761 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8821 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8881 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8941 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9001 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     9061 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9121 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9181 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9241 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     9301 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     9361 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9421 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9481 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9541 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9601 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9661 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     9721 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     9781 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     9841 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     9901 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     9961 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
    10021 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
    10081 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
    10141 cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
//
