LOCUS       V022856                10860 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022856
VERSION     V022856
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4978
                     /label="DPP9(NM_139159)"
                     /note="DPP9(NM_139159)"
                     /gene="DPP9"
     CDS             4985..5014
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     promoter        5058..5269
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5282..5550
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5585..6292
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             6302..6355
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6356..6955
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6956..7543
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7617..7850
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7922..8043
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8062..8197
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8235..8251)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8259..8275
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8283..8313)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8328..8349
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8637..9225)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9396..10256)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10257..10361)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10835..10851
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgcggaa ggttaagaaa ctgcgcctgg acaaggagaa
     2341 caccggaagt tggagaagct tctcgctgaa ttccgagggg gctgagagga tggccaccac
     2401 cgggacccca acggccgacc gaggcgacgc agccgccaca gatgacccgg ccgcccgctt
     2461 ccaggtgcag aagcactcgt gggacgggct ccggagcatc atccacggca gccgcaagta
     2521 ctcgggcctc attgtcaaca aggcgcccca cgacttccag tttgtgcaga agacggatga
     2581 gtctgggccc cactcccacc gcctctacta cctgggaatg ccatatggca gccgagagaa
     2641 ctccctcctc tactctgaga ttcccaagaa ggtccggaaa gaggctctgc tgctcctgtc
     2701 ctggaagcag atgctggatc atttccaggc cacgccccac catggggtct actctcggga
     2761 ggaggagctg ctgagggagc ggaaacgcct gggggtcttc ggcatcacct cctacgactt
     2821 ccacagcgag agtggcctct tcctcttcca ggccagcaac agcctcttcc actgccgcga
     2881 cggcggcaag aacggcttca tggtgtcccc tatgaaaccg ctggaaatca agacccagtg
     2941 ctcagggccc cggatggacc ccaaaatctg ccctgccgac cctgccttct tctccttcat
     3001 caataacagc gacctgtggg tggccaacat cgagacaggc gaggagcggc ggctgacctt
     3061 ctgccaccaa ggtttatcca atgtcctgga tgaccccaag tctgcgggtg tggccacctt
     3121 cgtcatacag gaagagttcg accgcttcac tgggtactgg tggtgcccca cagcctcctg
     3181 ggaaggttca gagggcctca agacgctgcg aatcctgtat gaggaagtcg atgagtccga
     3241 ggtggaggtc attcacgtcc cctctcctgc gctagaagaa aggaagacgg actcgtatcg
     3301 gtaccccagg acaggcagca agaatcccaa gattgccttg aaactggctg agttccagac
     3361 tgacagccag ggcaagatcg tctcgaccca ggagaaggag ctggtgcagc ccttcagctc
     3421 gctgttcccg aaggtggagt acatcgccag ggccgggtgg acccgggatg gcaaatacgc
     3481 ctgggccatg ttcctggacc ggccccagca gtggctccag ctcgtcctcc tccccccggc
     3541 cctgttcatc ccgagcacag agaatgagga gcagcggcta gcctctgcca gagctgtccc
     3601 caggaatgtc cagccgtatg tggtgtacga ggaggtcacc aacgtctgga tcaatgttca
     3661 tgacatcttc tatcccttcc cccaatcaga gggagaggac gagctctgct ttctccgcgc
     3721 caatgaatgc aagaccggct tctgccattt gtacaaagtc accgccgttt taaaatccca
     3781 gggctacgat tggagtgagc ccttcagccc cggggaagat gaatttaagt gccccattaa
     3841 ggaagagatt gctctgacca gcggtgaatg ggaggttttg gcgaggcacg gctccaagat
     3901 ctgggtcaat gaggagacca agctggtgta cttccagggc accaaggaca cgccgctgga
     3961 gcaccacctc tacgtggtca gctatgaggc ggccggcgag atcgtacgcc tcaccacgcc
     4021 cggcttctcc catagctgct ccatgagcca gaacttcgac atgttcgtca gccactacag
     4081 cagcgtgagc acgccgccct gcgtgcacgt ctacaagctg agcggccccg acgacgaccc
     4141 cctgcacaag cagccccgct tctgggctag catgatggag gcagccagct gccccccgga
     4201 ttatgttcct ccagagatct tccatttcca cacgcgctcg gatgtgcggc tctacggcat
     4261 gatctacaag ccccacgcct tgcagccagg gaagaagcac cccaccgtcc tctttgtata
     4321 tggaggcccc caggtgcagc tggtgaataa ctccttcaaa ggcatcaagt acttgcggct
     4381 caacacactg gcctccctgg gctacgccgt ggttgtgatt gacggcaggg gctcctgtca
     4441 gcgagggctt cggttcgaag gggccctgaa aaaccaaatg ggccaggtgg agatcgagga
     4501 ccaggtggag ggcctgcagt tcgtggccga gaagtatggc ttcatcgacc tgagccgagt
     4561 tgccatccat ggctggtcct acgggggctt cctctcgctc atggggctaa tccacaagcc
     4621 ccaggtgttc aaggtggcca tcgcgggtgc cccggtcacc gtctggatgg cctacgacac
     4681 agggtacact gagcgctaca tggacgtccc tgagaacaac cagcacggct atgaggcggg
     4741 ttccgtggcc ctgcacgtgg agaagctgcc caatgagccc aaccgcttgc ttatcctcca
     4801 cggcttcctg gacgaaaacg tgcacttttt ccacacaaac ttcctcgtct cccaactgat
     4861 ccgagcaggg aaaccttacc agctccagat ctaccccaac gagagacaca gtattcgctg
     4921 ccccgagtcg ggcgagcact atgaagtcac gttgctgcac tttctacagg aatacctcgg
     4981 atccgagcag aagctgatct cagaggagga cctgtagcgg ccgcgaagga tctgcgatcg
     5041 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     5101 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     5161 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     5221 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
     5281 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
     5341 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
     5401 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
     5461 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
     5521 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc
     5581 caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     5641 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     5701 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     5761 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     5821 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     5881 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     5941 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     6001 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     6061 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     6121 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     6181 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     6241 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agggatctcg
     6301 cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac
     6361 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     6421 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     6481 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     6541 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     6601 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     6661 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     6721 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     6781 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     6841 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     6901 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca
     6961 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     7021 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     7081 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     7141 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     7201 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     7261 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     7321 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     7381 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     7441 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctccgcct
     7501 tcgccctcag acgagtcgga tctccctttg gccgcctccc cgcctggtac ctttaagacc
     7561 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7621 agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg
     7681 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7741 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7801 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7861 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     7921 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7981 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8041 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg
     8101 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     8161 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca
     8221 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8281 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     8341 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8401 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8461 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8521 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8581 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8641 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8701 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8761 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8821 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8881 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8941 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9001 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9061 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9121 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9181 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9241 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9301 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9361 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9421 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9481 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9541 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9601 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9661 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9721 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9781 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9841 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9901 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9961 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    10021 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10081 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10141 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10201 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10261 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10321 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10381 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
    10441 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
    10501 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
    10561 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
    10621 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
    10681 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
    10741 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
    10801 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
