LOCUS       V025611                12083 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025611
VERSION     V025611
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6126
                     /label="DHX9(NM_001357)"
                     /note="DHX9(NM_001357)"
                     /gene="DHX9"
     CDS             6133..6198
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6242..6453
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6466..6734
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6763..6772
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6793..7455
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7525..7578
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7579..8178
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8179..8766
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8840..9073
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9145..9266
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9285..9420
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9458..9474)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9482..9498
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9506..9536)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9551..9572
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9860..10448)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10619..11479)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11480..11584)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12058..12074
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gtgacgttaa aaattttctg
     2341 tatgcctggt gtggcaaaag gaagatgacc ccatcctatg aaattagagc agtggggaac
     2401 aaaaacaggc agaaattcat gtgtgaggtt caggtggaag gttataatta cactggcatg
     2461 ggaaattcca ccaataaaaa agatgcacaa agcaatgctg ccagagactt tgttaactat
     2521 ttggttcgaa taaatgaaat aaagagtgaa gaagttccag cttttggggt agcatctccg
     2581 cccccactta ctgatactcc tgacactaca gcaaatgctg aaggagattt accaacaacc
     2641 atgggaggac ctcttcctcc acatctggct ctcaaagcag aaaataattc tgaggtaggg
     2701 gcctctggct atggtgttcc tgggcccacc tgggaccgag gagccaactt gaaggattac
     2761 tactcaagaa aggaagaaca agaagtgcaa gcgactctag aatcagaaga agtggattta
     2821 aatgctgggc ttcatggaaa ctggaccttg gaaaatgcta aagctcgtct aaaccaatat
     2881 tttcagaaag aaaagatcca aggagaatat aagtacaccc aagtgggtcc tgatcacaac
     2941 aggagcttta ttgcagaaat gaccatttat atcaagcagc tgggcagaag gatttttgca
     3001 cgagaacatg gatcaaataa gaaattggca gcacagtcct gtgccctgtc acttgtcaga
     3061 caactgtacc atcttggagt ggttgaagct tactccggac ttacaaagaa gaaggaagga
     3121 gagacagtgg agccttacaa agtaaacctc tctcaagatt tagagcatca gctgcaaaac
     3181 atcattcaag agctaaatct tgagattttg cccccgcctg aagatccttc tgtgccagtt
     3241 gcactcaaca ttggcaaatt ggctcagttc gaaccatctc agcgacaaaa ccaagtgggt
     3301 gtggttcctt ggtcacctcc acaatccaac tggaatcctt ggactagtag caacattgat
     3361 gaggggcctc tggcttttgc tactccagag caaataagca tggacctcaa gaatgaattg
     3421 atgtaccagt tggaacagga tcatgatttg caagcaatct tgcaggagag agagttactg
     3481 cctgtgaaga aatttgaaag tgagattctg gaagcaatca gccaaaattc agttgtcatt
     3541 attagagggg ctactggatg tgggaaaacc acacaggttc cccagttcat tctagatgac
     3601 tttatccaga atgaccgagc agcagagtgt aacatcgtag taactcagcc cagaagaatc
     3661 agtgcggttt ctgtggcaga gcgagttgca tttgaaagag gagaagagcc tggaaaaagc
     3721 tgtggctaca gcgttcgatt tgagtctata cttcctcgtc ctcatgccag tataatgttt
     3781 tgtactgtag gtgtgctcct gagaaaatta gaagcaggca ttcgaggaat cagtcatgta
     3841 attgtagatg aaatacatga aagagatatt aatactgact tccttttggt agtactgcgt
     3901 gatgttgttc aggcttatcc tgaagttcgc attgttctta tgtctgctac tattgatacc
     3961 agcatgtttt gtgaatattt cttcaattgc cccatcattg aagtttatgg gaggacttac
     4021 ccagttcaag aatattttct ggaagactgc attcagatga cccactttgt tcctccacca
     4081 aaagacaaaa agaagaagga taaggatgat gatggtggtg aggatgatga tgcaaattgc
     4141 aacttgatct gtggtgatga atatggtcca gaaacaaggt tgagcatgtc tcaattgaac
     4201 gaaaaggaaa ctccttttga actcatcgag gctctactta agtacattga aacccttaat
     4261 gttcctggag ctgtgttggt ttttttgcct ggctggaatc tgatttatac tatgcagaag
     4321 catttggaaa tgaatccaca ttttggaagc catcggtatc agattctacc cctgcattct
     4381 cagattcctc gagaggaaca gcgcaaagtg tttgatccag taccagttgg agtaaccaag
     4441 gttattttgt ccacaaatat tgctgaaaca agcattacca taaacgatgt tgtttatgtc
     4501 attgactcct gcaagcagaa agtgaaactc ttcactgctc acaacaatat gaccaactat
     4561 gctaccgtat gggcatcaaa aacaaacctt gagcaacgga aagggcgagc tggccgagta
     4621 cggcctggat tctgctttca cctgtgcagc cgagctcgtt ttgagagact tgaaacccac
     4681 atgacaccag agatgttccg aacaccattg catgaaattg ctcttagcat aaaacttctg
     4741 cgtctaggag gaattggcca atttctggcc aaagcaattg aacctccccc tttggatgct
     4801 gtgattgaag cagaacacac tcttagagag cttgatgcat tagatgccaa tgatgagttg
     4861 actcctttgg gacgaatcct ggctaaactc cccattgagc ctcgttttgg caaaatgatg
     4921 ataatggggt gtattttcta cgtgggagat gctatctgta ccattgctgc tgctacctgc
     4981 tttccagagc ctttcatcaa tgaaggaaag cggctgggct atatccatcg aaattttgct
     5041 ggaaacagat tttctgatca cgtagccctt ttatcagtat tccaagcctg ggatgatgct
     5101 agaatgggtg gagaagaagc agagatacgt ttttgtgagc acaaaagact taatatggct
     5161 acactaagaa tgacttggga agccaaagtt cagctcaaag agattttgat taattctggg
     5221 tttccagaag attgtttgtt gacacaagtg tttactaaca ctggaccaga taataatttg
     5281 gatgttgtta tctccctcct ggcctttggt gtgtacccca atgtatgcta tcataaggaa
     5341 aagaggaaga ttctcaccac tgaagggcgt aatgcactta tccacaaatc atctgttaat
     5401 tgtcctttta gtagccaaga catgaagtac ccatctccct tctttgtatt tggtgaaaag
     5461 attcgaactc gagccatctc tgctaaaggc atgactttag tcacccccct gcagttgctt
     5521 ctctttgcct ccaagaaagt ccaatctgat gggcagattg tgcttgtaga tgactggatt
     5581 aaactgcaaa tatctcatga agctgctgcc tgtatcactg gtctccgggc agccatggag
     5641 gctttggttg ttgaagtaac caaacaacct gctatcatca gccagttgga ccccgtaaat
     5701 gaacgtatgc tgaacatgat ccgtcagatc tctagaccct cagctgctgg tatcaacctt
     5761 atgattggca gtacacggta tggagatggt ccacgtcctc ccaagatggc ccgatacgac
     5821 aatggaagcg gatatagaag gggaggttct agttacagtg gtggaggcta tggcggtggc
     5881 tatagcagtg gaggctatgg tagcggaggc tatggtggca gcgccaactc ctttcgggca
     5941 ggatatggtg caggtgttgg tggaggctat agaggagttt cccgaggtgg ctttagaggc
     6001 aactctggag gagactacag agggcctagt ggaggctaca gaggatctgg gggattccag
     6061 cgaggaggtg gtaggggggc ctatggaact ggctactttg gacagggaag aggaggtggc
     6121 ggctatggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     6181 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     6241 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     6301 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     6361 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     6421 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     6481 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     6541 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     6601 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     6661 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     6721 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     6781 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     6841 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     6901 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     6961 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     7021 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     7081 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     7141 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     7201 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     7261 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     7321 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     7381 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     7441 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     7501 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     7561 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     7621 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     7681 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     7741 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     7801 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     7861 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     7921 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     7981 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     8041 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     8101 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     8161 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     8221 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     8281 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     8341 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     8401 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     8461 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     8521 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     8581 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     8641 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8701 cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttggccgcct
     8761 ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     8821 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt
     8881 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8941 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     9001 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     9061 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     9121 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     9181 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9241 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     9301 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     9361 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     9421 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa
     9481 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     9541 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     9601 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     9661 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     9721 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     9781 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     9841 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     9901 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     9961 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    10021 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    10081 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    10141 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    10201 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    10261 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
    10321 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    10381 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    10441 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    10501 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    10561 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    10621 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    10681 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    10741 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    10801 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    10861 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    10921 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    10981 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    11041 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    11101 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    11161 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    11221 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    11281 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    11341 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    11401 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    11461 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    11521 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    11581 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
    11641 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
    11701 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
    11761 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
    11821 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
    11881 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
    11941 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
    12001 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
    12061 aaacgacggc cagtgccaag ctg
//
