LOCUS       V031845                11125 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031845
VERSION     V031845
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5151
                     /label="DGKZ(NM_201532)"
                     /note="DGKZ(NM_201532)"
                     /gene="DGKZ"
     CDS             5158..5223
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5267..5478
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5491..5759
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5788..5797
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5818..6480
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6550..6603
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6604..7203
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7204..7792
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7866..8099
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8171..8292
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8332..8467
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8500..8516)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8524..8540
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8548..8578)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8593..8614
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8902..9490)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9661..10521)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10522..10626)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11100..11116
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ccgagggaca gggaggagga
     2341 ggacagcggt gggactgggc cggcggaggt cgggccgccg aggaggaagt ggtgaggagg
     2401 agatgtagaa ggggagagga ggctcaggtg gcccagcctt ggcccgaggg aagtcgggga
     2461 actgccgccg gccctcctgt agaagagaga tttaggcagt tgcatcttag aaagcaggtg
     2521 tcatacagaa aagcaattac caaatcaggc ctgcagcacc tggccccccc tcctcccaca
     2581 cccggagccc catgcagcga gtcagagcgg cagatccgga gtacagtgga ctggagcgag
     2641 tcagcgacat atggggagca catctggttc gagaccaacg tgtccgggga cttctgctac
     2701 gttggggagc agtactgtgt agccaggatg ctgaagtcag tgtctcgaag aaagtgcgca
     2761 gcctgcaaga ttgtggtgca cacgccctgc atcgagcagc tggagaagat aaatttccgc
     2821 tgtaagccgt ccttccgtga atcaggctcc aggaatgtcc gcgagccaac ctttgtacgg
     2881 caccactggg tacacagacg acgccaggac ggcaagtgtc ggcactgtgg gaagggattc
     2941 cagcagaagt tcaccttcca cagcaaggag attgtggcca tcagctgctc gtggtgcaag
     3001 caggcatacc acagcaaggt gtcctgcttc atgctgcagc agatcgagga gccgtgctcg
     3061 ctgggggtcc acgcagccgt ggtcatcccg cccacctgga tcctccgcgc ccggaggccc
     3121 cagaatactc tgaaagcaag caagaagaag aagagggcat ccttcaagag gaagtccagc
     3181 aagaaagggc ctgaggaggg ccgctggaga cccttcatca tcaggcccac cccctccccg
     3241 ctcatgaagc ccctgctggt gtttgtgaac cccaagagtg ggggcaacca gggtgcaaag
     3301 atcatccagt ctttcctctg gtatctcaat ccccgacaag tcttcgacct gagccaggga
     3361 gggcccaagg aggcgctgga gatgtaccgc aaagtgcaca acctgcggat cctggcgtgc
     3421 gggggcgacg gcacggtggg ctggatcctc tccaccctgg accagctacg cctgaagccg
     3481 ccaccccctg ttgccatcct gcccctgggt actggcaacg acttggcccg aaccctcaac
     3541 tggggtgggg gctacacaga tgagcctgtg tccaagatcc tctcccacgt ggaggagggg
     3601 aacgtggtac agctggaccg ctgggacctc cacgctgagc ccaaccccga ggcagggcct
     3661 gaggaccgag atgaaggcgc caccgaccgg ttgcccctgg atgtcttcaa caactacttc
     3721 agcctgggct ttgacgccca cgtcaccctg gagttccacg agtctcgaga ggccaaccca
     3781 gagaaattca acagccgctt tcggaataag atgttctacg ccgggacagc tttctctgac
     3841 ttcctgatgg gcagctccaa ggacctggcc aagcacatcc gagtggtgtg tgatggaatg
     3901 gacttgactc ccaagatcca ggacctgaaa ccccagtgtg ttgttttcct gaacatcccc
     3961 aggtactgtg cgggcaccat gccctggggc caccctgggg agcaccacga ctttgagccc
     4021 cagcggcatg acgacggcta cctcgaggtc attggcttca ccatgacgtc gttggccgcg
     4081 ctgcaggtgg gcggacacgg cgagcggctg acgcagtgtc gcgaggtggt gctcaccaca
     4141 tccaaggcca tcccggtgca ggtggatggc gagccctgca agcttgcagc ctcacgcatc
     4201 cgcatcgccc tgcgcaacca ggccaccatg gtgcagaagg ccaagcggcg gagcgccgcc
     4261 cccctgcaca gcgaccagca gccggtgcca gagcagttgc gcatccaggt gagtcgcgtc
     4321 agcatgcacg actatgaggc cctgcactac gacaaggagc agctcaagga ggcctctgtg
     4381 ccgctgggca ctgtggtggt cccaggagac agtgacctag agctctgccg tgcccacatt
     4441 gagagactcc agcaggagcc cgatggtgct ggagccaagt ccccgacatg ccagaaactg
     4501 tcccccaagt ggtgcttcct ggacgccacc actgccagcc gcttctacag gatcgaccga
     4561 gcccaggagc acctcaacta tgtgactgag atcgcacagg atgagattta tatcctggac
     4621 cctgagctgc tgggggcatc ggcccggcct gacctcccaa cccccacttc ccctctcccc
     4681 acctcaccct gctcacccac gccccggtca ctgcaagggg atgctgcacc ccctcaaggt
     4741 gaagagctga ttgaggctgc caagaggaac gacttctgta agctccagga gctgcaccga
     4801 gctgggggcg acctcatgca ccgagacgag cagagtcgca cgctcctgca ccacgcagtc
     4861 agcactggca gcaaggatgt ggtccgctac ctgctggacc acgccccccc agagatcctt
     4921 gatgcggtgg aggaaaacgg ggagacctgt ttgcaccaag cagcggccct gggccagcgc
     4981 accatctgcc actacatcgt ggaggccggg gcctcgctca tgaagacaga ccagcagggc
     5041 gacactcccc ggcagcgggc tgagaaggct caggacaccg agctggccgc ctacctggag
     5101 aaccggcagc actaccagat gatccagcgg gaggaccagg agacggctgt gggatccgac
     5161 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac
     5221 aagtagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca
     5281 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga
     5341 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     5401 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     5461 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc
     5521 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt
     5581 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc
     5641 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg
     5701 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc
     5761 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc
     5821 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg
     5881 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa
     5941 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac
     6001 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc
     6061 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc
     6121 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc
     6181 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg
     6241 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac
     6301 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc
     6361 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc
     6421 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc
     6481 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc
     6541 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc
     6601 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     6661 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     6721 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     6781 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     6841 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     6901 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     6961 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     7021 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     7081 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     7141 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     7201 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7261 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7321 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7381 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7441 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7501 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7561 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     7621 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     7681 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     7741 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc
     7801 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     7861 gggactggaa gggctaattc actcccaacg aagataagat ctgctttttg cttgtactgg
     7921 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7981 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8041 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8101 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     8161 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     8221 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     8281 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     8341 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     8401 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     8461 ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg
     8521 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8581 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8641 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8701 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8761 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8821 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8881 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8941 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9001 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9061 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9121 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9181 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9241 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9301 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     9361 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9421 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9481 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9541 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9601 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9661 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9721 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9781 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9841 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9901 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9961 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    10021 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    10081 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    10141 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    10201 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    10261 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    10321 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    10381 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10441 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10501 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10561 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10621 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
    10681 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
    10741 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    10801 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
    10861 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
    10921 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
    10981 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
    11041 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    11101 taaaacgacg gccagtgcca agctg
//
