LOCUS       V029644                11226 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029644
VERSION     V029644
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2285..3511
                     /label="DEPTOR(NM_022783)"
                     /note="DEPTOR(NM_022783)"
                     /gene="DEPTOR"
     CDS             3518..3607
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     CDS             3617..3673
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3686..5338
                     /EC_number=""
                     /codon_start=1
                     /experiment=""
                     /gene="luc"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL*"
                     /label="luciferase"
     promoter        5385..5596
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5609..5877
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5906..5915
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5936..6598
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6668..6721
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6722..7321
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7322..7909
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7983..8216
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8288..8409
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8428..8563
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8601..8617)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8625..8641
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8649..8679)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8694..8715
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9003..9591)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9762..10622)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10623..10727)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11201..11217
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 caccatggag gagggcggca gcactggcag tgctggcagt gacagcagca ccagcgggag
     2341 tggcggggcg cagcaaaggg agctggagcg catggctgag gtcttggtca ccggggaaca
     2401 gctacggctc aggctgcacg aagaaaaggt tattaaagat agacgtcatc atctcaagac
     2461 ctacccaaac tgttttgtcg caaaagaact gattgactgg ctgattgaac acaaagaggc
     2521 ttctgacaga gagacggcaa ttaaactcat gcagaaatta gcagaccggg gcattattca
     2581 ccatgtgtgt gatgagcata aggaattcaa ggatgtcaaa ctcttctacc gctttagaaa
     2641 ggatgacggc accttcccat tggataatga agtgaaggcc tttatgagag gacagaggct
     2701 atatgaaaag ctgatgagcc ctgaaaacac actcctgcag cccagggagg aggaaggggt
     2761 caagtatgag cgcaccttca tggcatctga attcctggac tggctggttc aggaaggtga
     2821 ggccaccacg aggaaagagg cagagcagct ttgccaccgg cttatggagc atggcatcat
     2881 ccagcatgtg tccaacaagc acccatttgt ggacagcaat cttctctacc agttcagaat
     2941 gaacttccgg cggaggcgaa gactgatgga gctgctcaat gaaaagtccc cctcctccca
     3001 ggaaactcat gacagtccct tctgcctgag gaagcagagc catgacaatc ggaaatctac
     3061 cagctttatg tcagtgagcc ccagcaagga gatcaagatc gtgtctgcag tgaggagaag
     3121 cagcatgagc agctgtggca gcagcggcta cttcagcagc agccccaccc tcagcagcag
     3181 cccccctgtg ctctgcaacc ccaagtccgt gctgaagaga cctgtcacct ctgaggaact
     3241 ccttactccc ggggctccgt atgcaaggaa gacattcacg attgttggtg acgcggttgg
     3301 ctggggtttt gtggtgcgag gaagtaagcc atgccacatc caggctgtag accccagtgg
     3361 ccctgcagcc gcagcaggaa tgaaggtctg tcagtttgtc gtctctgtca acgggctcaa
     3421 tgtcctgcat gtagactacc ggaccgtgag caatctgatt ctgacgggcc cacggacgat
     3481 tgtcatggaa gtcatggagg agttagagtg cctcgaggaa caaaaactaa tatcagagga
     3541 agatttggaa cagaaactaa tcagtgaaga agatttagag caaaagttaa tttccgagga
     3601 ggacttaggc agcggcgcca ccaacttcag cctgctgaag caggccggcg acgtggagga
     3661 gaaccccggc cccgaattcg ccaccatgga agacgccaaa aacataaaga aaggcccggc
     3721 gccattctat cctcttgagg atggaaccgc tggagagcaa ctgcataagg ctatgaagag
     3781 atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg tgaacatcac
     3841 gtacgcggaa tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct
     3901 gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt
     3961 gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg
     4021 tgaattgctc aacagtatga acatttcgca gcctaccgta gtgtttgttt ccaaaaaggg
     4081 gttgcaaaaa attttgaacg tgcaaaaaaa attaccaata atccagaaaa ttattatcat
     4141 ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct
     4201 acctcccggt tttaatgaat acgattttgt accagagtcc tttgatcgtg acaaaacaat
     4261 tgcactgata atgaactcct ctggatctac tgggttacct aagggtgtgg cccttccgca
     4321 tagaactgcc tgcgtcagat tctcgcatgc cagagatcct atttttggca atcaaatcat
     4381 tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac
     4441 actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct
     4501 gtttttacga tcccttcagg attacaaaat tcaaagtgcg ttgctagtac caaccctatt
     4561 ttcattcttc gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat
     4621 tgcttctggg ggcgcacctc tttcgaaaga agtcggggaa gcggttgcaa aacgcttcca
     4681 tcttccaggg atacgacaag gatatgggct cactgagact acatcagcta ttctgattac
     4741 acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa
     4801 ggttgtggat ctggataccg ggaaaacgct gggcgttaat cagagaggcg aattatgtgt
     4861 cagaggacct atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat
     4921 tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag acgaacactt
     4981 cttcatagtt gaccgcttga agtctttaat taaatacaaa ggataccagg tggcccccgc
     5041 tgaattggag tcgatattgt tacaacaccc caacatcttc gacgcgggcg tggcaggtct
     5101 tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac
     5161 gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt
     5221 gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc
     5281 aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagtcca aattgtgagg
     5341 atccgcggcc gcaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc
     5401 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag
     5461 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     5521 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     5581 tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg
     5641 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg
     5701 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct
     5761 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac
     5821 cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa
     5881 gctgtgaccg gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc
     5941 catggagatc gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg
     6001 cggcggagag ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg
     6061 cgccctgacc ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt
     6121 cggcacctac cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta
     6181 caccaacacc cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta
     6241 ccgctacgag gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga
     6301 ggacagcgtg atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca
     6361 ccccatgggc gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg
     6421 cggctactac agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag
     6481 catcctgcag aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa
     6541 caccgagctg ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag
     6601 atcccgcgct cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg
     6661 atctcgcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc
     6721 tatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     6781 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     6841 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     6901 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     6961 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     7021 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     7081 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     7141 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     7201 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     7261 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     7321 aaatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     7381 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     7441 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     7501 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     7561 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     7621 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7681 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     7741 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7801 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7861 ccgccttcgc cctcagacga gtcggatctc cctttggccg cctccccgcc tggtaccttt
     7921 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     7981 actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt gtactgggtc
     8041 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     8101 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     8161 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     8221 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     8281 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8341 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8401 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat
     8461 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     8521 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac
     8581 ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     8641 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8701 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8761 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     8821 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8881 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     8941 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     9001 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     9061 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     9121 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     9181 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9241 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     9301 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     9361 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9421 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     9481 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     9541 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     9601 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9661 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9721 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9781 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9841 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9901 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9961 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    10021 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    10081 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    10141 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    10201 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    10261 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    10321 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    10381 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    10441 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    10501 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    10561 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    10621 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10681 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10741 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
    10801 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
    10861 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
    10921 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
    10981 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
    11041 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
    11101 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
    11161 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
    11221 aagctg
//
